##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631941.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308548
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33010747112281	33.0	31.0	34.0	31.0	34.0
2	32.513651036467586	34.0	31.0	34.0	31.0	34.0
3	32.589969794002876	34.0	31.0	34.0	31.0	34.0
4	36.10569506203249	37.0	35.0	37.0	35.0	37.0
5	35.99108404527011	37.0	35.0	37.0	35.0	37.0
6	36.04268379636232	37.0	35.0	37.0	35.0	37.0
7	36.03462670313857	37.0	35.0	37.0	35.0	37.0
8	36.01171292635181	37.0	35.0	37.0	35.0	37.0
9	37.82320741019225	39.0	38.0	39.0	35.0	39.0
10	37.71880550189922	39.0	37.0	39.0	35.0	39.0
11	37.80650336414431	39.0	38.0	39.0	35.0	39.0
12	37.72476891764004	39.0	37.0	39.0	35.0	39.0
13	37.78688567094909	39.0	38.0	39.0	35.0	39.0
14	39.11110751001465	40.0	38.0	41.0	36.0	41.0
15	39.107299350506246	40.0	38.0	41.0	36.0	41.0
16	39.04941532597845	40.0	38.0	41.0	36.0	41.0
17	39.00456006844964	40.0	38.0	41.0	36.0	41.0
18	39.00322154089477	40.0	38.0	41.0	36.0	41.0
19	39.021523393442834	40.0	39.0	41.0	36.0	41.0
20	38.996110815821204	40.0	38.0	41.0	36.0	41.0
21	38.9770149215033	40.0	38.0	41.0	36.0	41.0
22	38.945321311432906	40.0	38.0	41.0	35.0	41.0
23	38.882643867404745	40.0	38.0	41.0	35.0	41.0
24	38.89571476723233	40.0	38.0	41.0	35.0	41.0
25	38.82654562661239	40.0	38.0	41.0	35.0	41.0
26	38.74488248181807	40.0	38.0	41.0	35.0	41.0
27	38.62566926377743	40.0	38.0	41.0	35.0	41.0
28	38.6123812178332	40.0	38.0	41.0	35.0	41.0
29	38.5939043519971	40.0	38.0	41.0	35.0	41.0
30	38.54433994062512	40.0	38.0	41.0	35.0	41.0
31	38.48362329362044	40.0	38.0	41.0	34.0	41.0
32	38.42349002424258	40.0	38.0	41.0	34.0	41.0
33	38.3506909783891	40.0	38.0	41.0	34.0	41.0
34	38.311526893708596	40.0	38.0	41.0	34.0	41.0
35	38.2058707235179	40.0	38.0	41.0	34.0	41.0
36	38.170991223407704	40.0	38.0	41.0	34.0	41.0
37	38.11021299765353	40.0	37.0	41.0	33.0	41.0
38	38.07954029843007	40.0	37.0	41.0	33.0	41.0
39	38.01589379934403	40.0	37.0	41.0	33.0	41.0
40	37.71009372933871	40.0	37.0	41.0	33.0	41.0
41	37.7706256400949	40.0	37.0	41.0	33.0	41.0
42	37.74877490698368	40.0	37.0	41.0	33.0	41.0
43	37.24350182143459	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	2.0
11	1.0
12	0.0
13	1.0
14	1.0
15	0.0
16	2.0
17	1.0
18	3.0
19	5.0
20	14.0
21	29.0
22	73.0
23	116.0
24	228.0
25	368.0
26	566.0
27	903.0
28	1339.0
29	1854.0
30	2731.0
31	3750.0
32	5063.0
33	6838.0
34	10065.0
35	15035.0
36	22378.0
37	39398.0
38	88114.0
39	109668.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.301852548063835	16.373465392742784	12.221113084511973	28.10356897468141
2	21.433294009359972	18.718643452558435	32.53788713587513	27.310175402206465
3	21.27124466857669	19.78492811491243	28.86131169218404	30.082515524326848
4	15.69934013508433	15.390474091551395	33.78728755331423	35.12289822005004
5	17.022959150602176	33.060658309241994	33.03699910548764	16.87938343466819
6	35.089191957167124	33.508562687166986	14.997342390811156	16.404902964854738
7	30.969897714456096	27.4048122172239	19.59435809015129	22.030931978168713
8	27.85368889119359	30.57449732294489	19.69029129989499	21.881522485966524
9	28.473365570348857	12.415572293451909	17.06865706470306	42.04240507149617
10	19.789789595135925	23.02591493057806	29.349404306623278	27.83489116766273
11	39.49596173042768	19.27836187562389	18.6230343414963	22.60264205245213
12	24.243877775905208	23.052815121148086	25.787235697525183	26.916071405421523
13	34.56155930357676	17.287747773442057	20.61073155554403	27.539961367437154
14	23.96742160052893	19.244655612740967	23.054111515874354	33.73381127085575
15	29.846895782827954	23.294268638915177	19.996564553975393	26.862271024281476
16	26.106472898868248	23.48613505840258	21.491955870723515	28.915436172005656
17	27.155256232417646	23.59405991936425	21.399911845158613	27.850772003059493
18	26.506410671921387	20.951035171188924	23.08554908798631	29.45700506890338
19	28.719680568339452	21.605714507953383	22.564722506708843	27.109882416998328
20	29.125776216342352	20.658698160415884	22.667137690083877	27.548387933157887
21	28.798112449278555	21.455332719706497	21.47964012082399	28.26691471019096
22	29.22981189312522	22.67264736767051	20.577025292661112	27.520515446543165
23	28.789685883557826	22.075009398861763	21.345463266655432	27.78984145092498
24	28.803622126865186	21.357130819191827	21.585620389696256	28.253626664246728
25	28.37710826192359	21.376900838767387	22.662600308541943	27.583390590767078
26	28.632822121679606	22.11228074724192	22.273033693298935	26.981863437779534
27	28.404008452493617	21.49681735094702	22.843447372856087	27.25572682370328
28	26.694063808548428	22.192333121588863	22.252615476360244	28.86098759350247
29	27.944760620713794	22.74686596574925	22.330399159936217	26.977974253600735
30	27.268042573602813	21.10206515679894	23.73536694452727	27.89452532507098
31	27.980411475686118	21.867910341340732	22.64898816391615	27.502690019057003
32	25.915254676743977	21.79142305249102	22.93808418787352	29.35523808289148
33	26.70702775581109	21.90777447917342	23.853662963299065	27.53153480171643
34	26.98348393118737	22.035469359710643	23.45242879551966	27.528617913582327
35	26.50219738906102	23.262506968121656	24.019277389579578	26.216018253237745
36	27.07779664752324	21.976159300983962	23.604755175855946	27.341288875636856
37	26.441915034289643	22.003383590235558	23.827086871410607	27.727614504064196
38	26.501225093016323	21.123455669782334	25.106952564916966	27.268366672284376
39	25.223303991599362	20.699858692974836	25.959656196118598	28.117181119307205
40	25.20709905752103	21.020716387725734	27.164330995501512	26.60785355925172
41	23.702957076370613	20.16379947366374	27.77363651684665	28.359606933118997
42	22.382579047668433	21.174663261469853	28.60657012847272	27.836187562388996
43	22.06820332654887	20.538781648236252	28.41956518920881	28.973449836006065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	9.5
4	14.0
5	14.0
6	12.5
7	11.0
8	17.5
9	24.0
10	31.0
11	38.0
12	38.0
13	82.5
14	127.0
15	253.0
16	379.0
17	360.0
18	341.0
19	341.0
20	383.5
21	426.0
22	345.0
23	264.0
24	313.5
25	363.0
26	363.0
27	473.0
28	583.0
29	793.0
30	1003.0
31	1368.0
32	1733.0
33	1733.0
34	2609.0
35	3485.0
36	4100.0
37	4715.0
38	6123.0
39	7531.0
40	7531.0
41	8974.0
42	10417.0
43	12493.5
44	14570.0
45	17915.0
46	21260.0
47	21260.0
48	23558.0
49	25856.0
50	29098.0
51	32340.0
52	33410.5
53	34481.0
54	34481.0
55	32552.0
56	30623.0
57	28390.5
58	26158.0
59	24423.5
60	22689.0
61	22689.0
62	21874.5
63	21060.0
64	16847.5
65	12635.0
66	11174.5
67	9714.0
68	9714.0
69	8298.5
70	6883.0
71	6280.5
72	5678.0
73	5953.0
74	6228.0
75	6228.0
76	5263.0
77	4298.0
78	2918.0
79	1538.0
80	1031.0
81	524.0
82	524.0
83	400.0
84	276.0
85	212.0
86	148.0
87	107.5
88	67.0
89	67.0
90	52.5
91	38.0
92	30.5
93	23.0
94	12.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	308548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.07242324689968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.04613286737195	40.033275618449984
2	6.60705266753026	6.220199591610839
3	2.3278147728716108	3.287276466869943
4	1.30976093162661	2.4661448370312566
5	0.7536494949468688	1.7738054002975594
6	0.5667884869678848	1.6008064530013293
7	0.42970398176312663	1.4159045390302372
8	0.3193760815729398	1.2027044869390224
9	0.2511921667835986	1.0641801592029034
>10	1.974270942967598	18.38845807707822
>50	0.24910507180453054	8.250295431728812
>100	0.16102372044823243	13.089367416597158
>500	0.004128813344826473	1.207581522162741
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	728	0.23594384018045816	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	688	0.22297989291779563	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	648	0.2100159456551331	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	605	0.19607970234777083	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	540	0.17501328804594424	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	519	0.1682072157330464	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	468	0.15167818297315167	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	442	0.14325161725242103	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	426	0.138066038347356	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	413	0.13385275548699066	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	408	0.13223226207915786	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	402	0.1302876699897585	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	396	0.1283430779003591	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	384	0.12445389372156034	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	371	0.12024061086119502	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	342	0.11084174909576468	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	340	0.11019355173263155	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	321	0.10403567678286686	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	320	0.10371157810130029	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.240986815665634E-4	0.0	0.0	0.0	0.0
6	6.481973631331267E-4	0.0	0.0	0.0	0.0
7	6.481973631331267E-4	0.0	0.0	0.0	0.0
8	6.481973631331267E-4	0.0	0.0	0.0	0.0
9	6.481973631331267E-4	0.0	0.0	0.0	0.0
10	6.481973631331267E-4	0.0	0.0	0.0	0.0
11	6.481973631331267E-4	0.0	0.0	0.0	0.0
12	6.481973631331267E-4	0.0	0.0	0.0	0.0
13	6.481973631331267E-4	0.0	0.0	0.0	0.0
14	6.481973631331267E-4	0.0	0.0	0.0	0.0
15	6.481973631331267E-4	0.0	0.0	0.0	0.0
16	6.481973631331267E-4	0.0	0.0	0.0	0.0
17	9.722960446996901E-4	0.0	0.0	0.001620493407832817	0.0
18	9.722960446996901E-4	0.0	0.0	0.001620493407832817	0.0
19	9.722960446996901E-4	0.0	0.0	0.001620493407832817	0.0
20	9.722960446996901E-4	0.0	0.0	0.0019445920893993802	0.0
21	9.722960446996901E-4	0.0	0.0	0.003565085497232197	0.0
22	9.722960446996901E-4	0.0	0.0	0.005185578905065014	0.0
23	9.722960446996901E-4	0.0	0.0	0.006157874949764704	0.0
24	9.722960446996901E-4	0.0	0.0	0.008102467039164086	0.0
25	9.722960446996901E-4	0.0	0.0	0.009398861765430339	0.0
26	9.722960446996901E-4	0.0	0.0	0.013612144625795661	0.0
27	9.722960446996901E-4	0.0	0.0	0.03532675629075541	0.0
28	0.0012963947262662535	0.0	0.0	0.13061176867132504	0.0
29	0.0012963947262662535	0.0	0.0	0.27062239910808045	0.0
30	0.001620493407832817	0.0	0.0	0.4508212660590897	0.0
31	0.001620493407832817	0.0	0.0	0.9256258345541051	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGACC	45	1.075623E-7	32.88889	37
GTATTGT	25	0.0054901903	29.599998	1
AAATTTA	25	0.0054901903	29.599998	18
GGTATCA	415	0.0	28.53012	1
GTTCTAA	35	8.8546355E-4	26.42857	1
GCGGTAA	325	0.0	23.33846	23
ATTAGAG	80	2.7093847E-8	23.125	3
CGGTAAT	330	0.0	22.984848	24
AGCCGCG	350	0.0	22.72857	19
CACATGT	75	3.7224345E-7	22.2	28
TGAGTAT	75	3.7224345E-7	22.2	3
TAATACG	310	0.0	22.080645	27
GGTAATA	370	0.0	22.0	25
CATCAGA	245	0.0	21.897959	16
CGCGGTA	355	0.0	21.887323	22
GACACAT	85	5.149741E-8	21.764706	26
AGAAGAG	255	0.0	21.764706	20
AGTGACA	85	5.149741E-8	21.764706	23
TATTAGA	85	5.149741E-8	21.764706	2
TTTAACG	60	3.714922E-5	21.583334	34
>>END_MODULE