FastQCFastQC Report
Fri 10 Feb 2017
ERR1631940.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631940.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946029
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.20570193936972334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17450.18445523340193587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14410.15232091193821753No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11060.11690973532523843No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10190.10771339990634536No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9720.10274526468004681No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9510.10052545957893468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9950.029.9346731
TATAGGA358.8682305E-426.428572
TACACCG551.9021969E-523.5454545
GTACTAG400.001930935823.1250021
ATTTCGG1052.2580934E-819.3809529
GCGGTAA3500.019.02857223
GTTCTAA701.21890786E-418.51
AACTCCG3700.018.55
CGCGGTA3750.018.25333222
TATACAC3450.018.23188437
CTTATAC11200.018.16964137
GTATCAA16700.017.835332
GCAGTCG2200.017.659099
TCCGTGC4200.017.6190478
CGTGCCA4150.017.38554210
GTACTGG4050.017.3580251
AGTCGGT2250.017.26666611
CGGTAAT4000.016.65000224
GTGATTC2450.016.61224416
CCGCGGT4250.016.54117821
Produced by FastQC (version 0.11.5)