##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631940.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	946029
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61535217207929	34.0	31.0	34.0	31.0	34.0
2	32.79116919248776	34.0	31.0	34.0	31.0	34.0
3	32.88561767133989	34.0	31.0	34.0	31.0	34.0
4	36.29534929690316	37.0	37.0	37.0	35.0	37.0
5	36.220687737902324	37.0	35.0	37.0	35.0	37.0
6	36.26725819187361	37.0	37.0	37.0	35.0	37.0
7	36.252791404914646	37.0	37.0	37.0	35.0	37.0
8	36.242708204505355	37.0	37.0	37.0	35.0	37.0
9	38.0699693138371	39.0	38.0	39.0	37.0	39.0
10	38.00534127389329	39.0	38.0	39.0	35.0	39.0
11	38.07434761513653	39.0	38.0	39.0	37.0	39.0
12	38.01963047644417	39.0	38.0	39.0	35.0	39.0
13	38.0661734471142	39.0	38.0	39.0	37.0	39.0
14	39.46691591906802	40.0	39.0	41.0	37.0	41.0
15	39.46344879491009	41.0	39.0	41.0	37.0	41.0
16	39.43017497349447	40.0	39.0	41.0	37.0	41.0
17	39.402525715385046	40.0	39.0	41.0	37.0	41.0
18	39.415273738965716	41.0	39.0	41.0	37.0	41.0
19	39.446079348518914	41.0	39.0	41.0	37.0	41.0
20	39.433027951574424	41.0	39.0	41.0	37.0	41.0
21	39.40347706042838	40.0	39.0	41.0	37.0	41.0
22	39.386928941924616	40.0	39.0	41.0	37.0	41.0
23	39.32388753410308	40.0	39.0	41.0	36.0	41.0
24	39.33442949423326	41.0	39.0	41.0	36.0	41.0
25	39.276504208644766	40.0	39.0	41.0	36.0	41.0
26	39.22210207086675	40.0	39.0	41.0	36.0	41.0
27	39.12975500751034	40.0	39.0	41.0	36.0	41.0
28	39.11985890495957	40.0	39.0	41.0	35.0	41.0
29	39.0890955774083	40.0	39.0	41.0	35.0	41.0
30	39.044098013908666	40.0	39.0	41.0	35.0	41.0
31	38.957553098266544	40.0	38.0	41.0	35.0	41.0
32	38.9039627749255	40.0	38.0	41.0	35.0	41.0
33	38.848167445184025	40.0	38.0	41.0	35.0	41.0
34	38.81197827973561	40.0	38.0	41.0	35.0	41.0
35	38.74567375841544	40.0	38.0	41.0	35.0	41.0
36	38.69506325916013	40.0	38.0	41.0	35.0	41.0
37	38.656077139284314	40.0	38.0	41.0	35.0	41.0
38	38.61634051387431	40.0	38.0	41.0	35.0	41.0
39	38.55127379816052	40.0	38.0	41.0	35.0	41.0
40	38.24901139394247	40.0	38.0	41.0	34.0	41.0
41	38.30793982002666	40.0	38.0	41.0	34.0	41.0
42	38.287980601017516	40.0	38.0	41.0	34.0	41.0
43	37.832412114216375	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	1.0
18	5.0
19	12.0
20	22.0
21	65.0
22	143.0
23	260.0
24	440.0
25	732.0
26	1157.0
27	1876.0
28	2855.0
29	4411.0
30	6193.0
31	8585.0
32	11777.0
33	15874.0
34	23249.0
35	34824.0
36	53740.0
37	98456.0
38	219075.0
39	462273.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.32068995770743	17.705694011494362	12.991039386741843	26.982576644056365
2	19.501833453308514	19.872223790179795	34.577798355018714	26.04814440149298
3	20.195681104913273	21.105272671345173	29.886504536330282	28.812541687411276
4	15.031568799687959	16.244533729938514	34.51004144693239	34.21385602344114
5	16.201300382969233	33.91016554460804	33.72909287136018	16.159441201062545
6	33.53554700754417	34.79343656484104	15.700892890175671	15.970123537439127
7	29.626787339500165	29.077966954501395	20.833505103966157	20.461740602032286
8	26.717574196985506	32.60904264034189	20.092090200194708	20.581292962477896
9	27.35560960604802	14.052634750097512	18.856610103918587	39.73514553993588
10	18.434530019692843	24.944584151225808	31.184456290451983	25.43642953862937
11	37.28173237818291	20.783929456707988	20.422629750250785	21.51170841485832
12	22.406606985620947	24.63423425708937	27.84671505841787	25.11244369887181
13	32.811890544581615	18.80703445666042	22.56812423297806	25.812950765779906
14	23.309644841754324	20.446096261319685	24.489312695488195	31.7549462014378
15	27.55327796505181	25.66179260889465	21.571114627564274	25.213814798489263
16	25.00758433409547	25.136650144974414	23.463974148784022	26.391791372146095
17	25.27417235623855	24.957057341793963	23.735530306153404	26.033239995814085
18	25.374803520822297	23.339982178136186	25.017203489533617	26.268010811507892
19	26.634807178215468	23.74345818151452	25.004518888955836	24.617215751314177
20	27.393980522795815	23.059229685347912	24.538676932736735	25.008112859119542
21	25.894977849516245	24.130021384122475	23.839438325886416	26.135562440474867
22	26.423080053571297	24.451258893754844	23.464819788822542	25.66084126385132
23	26.02922320563112	23.831087630506044	24.374199945244808	25.765489218618033
24	26.150466846153762	23.543992837428874	24.269869105492536	26.03567121092482
25	26.147824221033396	23.616189355717427	24.649244367773083	25.5867420554761
26	25.903645659911064	24.146088544854333	24.316590717620706	25.633675077613898
27	25.724369971745055	23.765761937530456	24.79416592937426	25.715702161350233
28	25.185591562203697	24.347350874021835	24.299043686821438	26.168013876953033
29	25.590864550663877	24.783489723887957	24.225473003470295	25.400172721977864
30	25.449642664231227	24.10877467815469	24.997119538618794	25.444463118995298
31	25.98229018349332	23.95190845100943	24.401577541491857	25.664223824005393
32	24.2838221661281	24.0114203687202	24.820592180577975	26.88416528457373
33	24.561403508771928	23.61756352078002	25.91717590052736	25.90385706992069
34	25.038027375482148	23.401713900948067	25.56412118444572	25.996137539124064
35	24.79458874939352	24.165009740716194	25.71580786635505	25.32459364353524
36	24.908221629569496	23.419895161776225	25.98123313344517	25.69065007520911
37	24.897334014073564	22.901517818164134	25.94635048185626	26.254797685906034
38	24.21923640818622	22.557659437501385	26.90181802037781	26.321286133934585
39	23.807304004422697	22.041290488980785	27.80147331635711	26.34993219023941
40	23.707307069867838	22.666007067436624	28.152836752361715	25.47384911033383
41	22.670235267629216	22.077653010637093	29.008201651323585	26.243910070410102
42	21.733583219964718	22.515483140580255	29.340432481456702	26.41050115799833
43	21.219539781550036	22.591485039042144	29.32415391071521	26.86482126869261
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	29.5
2	30.0
3	71.5
4	113.0
5	113.0
6	159.5
7	206.0
8	224.0
9	242.0
10	374.0
11	506.0
12	506.0
13	958.5
14	1411.0
15	2625.0
16	3839.0
17	3864.5
18	3890.0
19	3890.0
20	4539.0
21	5188.0
22	5131.0
23	5074.0
24	5753.0
25	6432.0
26	6432.0
27	7489.5
28	8547.0
29	10745.0
30	12943.0
31	15213.5
32	17484.0
33	17484.0
34	20674.0
35	23864.0
36	26605.0
37	29346.0
38	33337.0
39	37328.0
40	37328.0
41	42031.5
42	46735.0
43	50902.5
44	55070.0
45	62765.5
46	70461.0
47	70461.0
48	74921.0
49	79381.0
50	84945.5
51	90510.0
52	90126.5
53	89743.0
54	89743.0
55	82391.0
56	75039.0
57	70242.5
58	65446.0
59	60705.0
60	55964.0
61	55964.0
62	51238.5
63	46513.0
64	40033.0
65	33553.0
66	29533.0
67	25513.0
68	25513.0
69	22219.5
70	18926.0
71	16230.0
72	13534.0
73	11270.0
74	9006.0
75	9006.0
76	7249.5
77	5493.0
78	4512.0
79	3531.0
80	2901.5
81	2272.0
82	2272.0
83	1795.5
84	1319.0
85	1062.5
86	806.0
87	625.0
88	444.0
89	444.0
90	339.5
91	235.0
92	145.0
93	55.0
94	31.0
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	946029.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.897106120104034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.15071056619841	50.457739380936616
2	6.722395301653792	7.7841446832227685
3	2.085996791800004	3.623195328631242
4	0.9884445863524198	2.289123244395536
5	0.587833049784753	1.7016916232146122
6	0.3985926380323539	1.3846416157710846
7	0.28863976018727444	1.1697984778230808
8	0.19699334438072913	0.9124275651652219
9	0.16884168586489046	0.8797900503615149
>10	1.2041508661974862	13.855556884912753
>50	0.12422412063567696	5.004681169771793
>100	0.07768099233046866	8.375647733987341
>500	0.004580247151487557	1.7917888686319852
>1k	9.160494302975115E-4	0.7697733731744208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1946	0.20570193936972334	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1745	0.18445523340193587	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1441	0.15232091193821753	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1106	0.11690973532523843	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1019	0.10771339990634536	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	972	0.10274526468004681	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	951	0.10052545957893468	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	0.0
3	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	0.0
4	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	0.0
5	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	0.0
6	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	0.0
7	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	0.0
8	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	1.0570500481486296E-4
9	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	1.0570500481486296E-4
10	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	1.0570500481486296E-4
11	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	1.0570500481486296E-4
12	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	1.0570500481486296E-4
13	1.0570500481486296E-4	0.0	0.0	1.0570500481486296E-4	1.0570500481486296E-4
14	1.0570500481486296E-4	0.0	0.0	3.171150144445889E-4	1.0570500481486296E-4
15	1.0570500481486296E-4	0.0	0.0	4.2282001925945186E-4	1.0570500481486296E-4
16	1.0570500481486296E-4	0.0	0.0	8.456400385189037E-4	1.0570500481486296E-4
17	1.0570500481486296E-4	0.0	0.0	0.0011627550529634927	1.0570500481486296E-4
18	2.1141000962972593E-4	1.0570500481486296E-4	0.0	0.0012684600577783556	2.1141000962972593E-4
19	2.1141000962972593E-4	1.0570500481486296E-4	0.0	0.0019026900866675335	2.1141000962972593E-4
20	2.1141000962972593E-4	1.0570500481486296E-4	0.0	0.002536920115556711	2.1141000962972593E-4
21	3.171150144445889E-4	1.0570500481486296E-4	0.0	0.003699675168520204	2.1141000962972593E-4
22	3.171150144445889E-4	1.0570500481486296E-4	0.0	0.005496660250372875	2.1141000962972593E-4
23	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.006553710298521504	2.1141000962972593E-4
24	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.007716465351484997	2.1141000962972593E-4
25	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.008667810394818764	2.1141000962972593E-4
26	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.01067620548630116	2.1141000962972593E-4
27	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.030548746391495397	2.1141000962972593E-4
28	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.1170154403300533	2.1141000962972593E-4
29	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.2580259167530805	2.1141000962972593E-4
30	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.43719589991427327	2.1141000962972593E-4
31	3.171150144445889E-4	2.1141000962972593E-4	0.0	0.9856991698985972	2.1141000962972593E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	995	0.0	29.934673	1
TATAGGA	35	8.8682305E-4	26.42857	2
TACACCG	55	1.9021969E-5	23.545454	5
GTACTAG	40	0.0019309358	23.125002	1
ATTTCGG	105	2.2580934E-8	19.38095	29
GCGGTAA	350	0.0	19.028572	23
GTTCTAA	70	1.21890786E-4	18.5	1
AACTCCG	370	0.0	18.5	5
CGCGGTA	375	0.0	18.253332	22
TATACAC	345	0.0	18.231884	37
CTTATAC	1120	0.0	18.169641	37
GTATCAA	1670	0.0	17.83533	2
GCAGTCG	220	0.0	17.65909	9
TCCGTGC	420	0.0	17.619047	8
CGTGCCA	415	0.0	17.385542	10
GTACTGG	405	0.0	17.358025	1
AGTCGGT	225	0.0	17.266666	11
CGGTAAT	400	0.0	16.650002	24
GTGATTC	245	0.0	16.612244	16
CCGCGGT	425	0.0	16.541178	21
>>END_MODULE