FastQCFastQC Report
Fri 10 Feb 2017
ERR1631939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954739
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.433940584809042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38520.4034610506117379No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31230.32710510411746035No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA26390.27641062112263143No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA22880.239646646884646No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA21360.2237260654482534No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19130.20036889663038798No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17310.18130609517365479No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16580.17366002645749257No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16530.17313632312076913No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15660.16402388506178128No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT14980.1569015196823425No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14620.15313085565793375No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC14260.14936019163352496No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC14110.14778908162335466No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13840.14496108360504809No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC13640.14286627025815432No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13640.14286627025815432No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13220.13846716222967742No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12880.13490597953995806No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12720.13323012886244304No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA12640.13239220352368553No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT12340.1292499835033449No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC12300.12883102083396614No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11760.123175024797353No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11660.12212761812390611No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11580.12128969278514862No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA11460.12003280477701236No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11410.11950910144028892No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11400.11940436077294424No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC11230.11762376942808454No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA11200.11730954742605046No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG11160.11689058475667172No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10830.11343414273429701No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10730.11238673606085013No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10640.11144407005474793No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC10510.11008244137926701No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG10470.10966347870988825No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC10110.10589281468547948No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9730.10191266932638135No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC9650.10107474398762385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.030.3233071
ATACACA1950.023.71794937
AAGGGCG400.001930949323.1255
ATACGGC1054.0017767E-1122.9047629
GATGCTA705.100428E-621.14285914
AACGACC1059.822543E-1021.14285737
CGGAATG450.00382534120.55555514
TTACTCG450.00382534120.55555529
TCGGACT450.00382534120.55555533
TTATACT555.1422167E-420.1818184
TAAACCG656.900564E-519.9230775
GTTATCG656.900564E-519.92307711
GTATCAA30100.019.9136222
CTTAAAC851.2448563E-619.5882343
TTTTACG851.2448563E-619.5882344
CAGTACT609.2352525E-418.54
AACAGTC500.007034030718.57
ACCCATC500.007034030718.58
TACCGCT701.21892255E-418.535
TATCCGG609.2352525E-418.510
Produced by FastQC (version 0.11.5)