##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631939.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954739
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24030022864888	33.0	31.0	34.0	30.0	34.0
2	32.417730919130776	34.0	31.0	34.0	31.0	34.0
3	32.49663415865488	34.0	31.0	34.0	31.0	34.0
4	36.04526996383305	37.0	35.0	37.0	35.0	37.0
5	35.906406881880805	37.0	35.0	37.0	35.0	37.0
6	35.9557062191866	37.0	35.0	37.0	35.0	37.0
7	35.9611024583682	37.0	35.0	37.0	35.0	37.0
8	35.94096292285116	37.0	35.0	37.0	35.0	37.0
9	37.71473460286005	39.0	37.0	39.0	35.0	39.0
10	37.60463330816066	39.0	37.0	39.0	35.0	39.0
11	37.69405146327949	39.0	37.0	39.0	35.0	39.0
12	37.60215619137796	39.0	37.0	39.0	35.0	39.0
13	37.68151819502503	39.0	37.0	39.0	35.0	39.0
14	38.93094133579962	40.0	38.0	41.0	36.0	41.0
15	38.92989392912618	40.0	38.0	41.0	36.0	41.0
16	38.867561710582684	40.0	38.0	41.0	36.0	41.0
17	38.85546730572439	40.0	38.0	41.0	36.0	41.0
18	38.89976422875781	40.0	38.0	41.0	36.0	41.0
19	38.920384523937955	40.0	38.0	41.0	36.0	41.0
20	38.905678934242765	40.0	38.0	41.0	36.0	41.0
21	38.86847714401527	40.0	38.0	41.0	35.0	41.0
22	38.82180679745983	40.0	38.0	41.0	35.0	41.0
23	38.760848776471896	40.0	38.0	41.0	35.0	41.0
24	38.76933172312014	40.0	38.0	41.0	35.0	41.0
25	38.703286447919275	40.0	38.0	41.0	35.0	41.0
26	38.60849614397233	40.0	38.0	41.0	35.0	41.0
27	38.465895915009234	40.0	38.0	41.0	34.0	41.0
28	38.461703146095424	40.0	38.0	41.0	34.0	41.0
29	38.411330216949345	40.0	38.0	41.0	34.0	41.0
30	38.34524199807487	40.0	38.0	41.0	34.0	41.0
31	38.23971787053844	40.0	38.0	41.0	34.0	41.0
32	38.174144975747296	40.0	38.0	41.0	34.0	41.0
33	38.085379354986024	40.0	37.0	41.0	34.0	41.0
34	38.040571297495966	40.0	37.0	41.0	33.0	41.0
35	37.94201242433796	40.0	37.0	41.0	33.0	41.0
36	37.87307002227834	40.0	37.0	41.0	33.0	41.0
37	37.81597588450875	40.0	37.0	41.0	33.0	41.0
38	37.77469549269486	40.0	37.0	41.0	33.0	41.0
39	37.675466279265855	40.0	37.0	41.0	33.0	41.0
40	37.35458381819534	40.0	36.0	41.0	32.0	41.0
41	37.38532206184099	39.0	36.0	41.0	33.0	41.0
42	37.318768794403496	39.0	36.0	41.0	33.0	41.0
43	36.78693443967409	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	8.0
19	33.0
20	66.0
21	145.0
22	276.0
23	500.0
24	850.0
25	1348.0
26	2118.0
27	3272.0
28	4841.0
29	6956.0
30	9632.0
31	13053.0
32	17162.0
33	23340.0
34	33144.0
35	48287.0
36	75379.0
37	141183.0
38	289297.0
39	283844.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.63217486663894	17.65121148292884	13.393503355367278	26.323110295064932
2	20.51146962677758	20.836479917548147	33.51795621630624	25.13409423936804
3	21.292206561164882	21.286236343126237	30.225747560328003	27.19580953538087
4	14.88218246033733	17.52447527544177	35.63099443931797	31.96234782490293
5	15.97347547340163	33.7660868572458	33.94205117838488	16.318386490967686
6	30.791766126658697	35.867813088184306	16.56128009854002	16.77914068661697
7	28.415724087944454	29.179911996891295	20.892725655912244	21.511638259252006
8	26.25261982594196	31.343120999561137	20.858265976355842	21.545993198141062
9	27.856094702321787	13.457395162447538	18.91972570514036	39.76678443009032
10	19.36173132133494	24.002266588041337	30.14970583583576	26.486296254787955
11	37.3172144429001	20.92058667342593	19.991327472743862	21.770871410930106
12	21.64444942544507	25.31875203589672	27.957064705642065	25.079733833016142
13	34.392645529301724	18.646876266707444	22.004128877106726	24.9563493268841
14	23.12380661102144	21.69231591042159	25.168344437589752	30.015533040967217
15	28.790276714369057	25.170648732271335	21.719757965265902	24.31931658809371
16	23.283640869389437	25.329959287302607	24.61583741734652	26.77056242596144
17	25.03993237942516	24.88145974973265	23.671705041901504	26.406902828940687
18	25.205422633829773	22.160401952785	25.490631471009355	27.143543942375874
19	26.66980190397585	23.505481602825483	25.64868513803249	24.176031355166177
20	28.376341597022854	21.793809617078594	24.97394575899801	24.855903026900545
21	26.06314395871542	23.45332075048783	24.205777704692068	26.277757586104684
22	26.39799987221639	24.264432478405094	23.870712309856412	25.46685533952211
23	26.359874269302917	23.16758820997152	24.970384576308287	25.502152944417272
24	26.023551986459125	22.951822435241464	25.109375441874693	25.915250136424717
25	26.18673794618215	23.393618570101356	25.426320701259712	24.993322782456776
26	25.988359122231312	24.167442620443914	24.7177500866729	25.126448170651877
27	25.633916703936887	23.189060046777183	26.27011151738852	24.90691173189741
28	24.951845478188282	24.458097972325422	24.420705554083366	26.169350995402933
29	25.447164094061307	24.86532968696157	24.383522617176006	25.30398360180112
30	25.37908266028726	23.423260178959904	26.191765498214696	25.005891662538136
31	26.122741398434545	23.55984200917738	24.978135385691797	25.339281206696278
32	23.726170188920744	23.659031421152797	25.456695494789678	27.15810289513679
33	23.975662458535787	23.325956098996688	27.06519792320205	25.633183519265472
34	24.43589295084835	23.343133568441218	26.270635220725246	25.95033825998519
35	24.101770222018793	24.65249665091716	26.445342653856187	24.800390473207862
36	24.58137773779012	23.387753092730055	26.85948725253708	25.17138191694275
37	25.069469247616365	23.18434671674667	26.203286971622614	25.54289706401435
38	24.09056297061291	22.578526696824998	27.075671989936517	26.255238342625574
39	23.624990704265773	21.70415160583154	28.392681141128623	26.278176548774063
40	23.570525557246537	22.682429438830926	28.684698121685614	25.062346882236923
41	22.039426482001886	22.06341209482382	29.581801937492862	26.315359485681427
42	20.52026784283453	22.835455553821514	30.15473338786831	26.489543215475642
43	20.019397971592237	23.46086207853665	29.566195578058508	26.95354437181261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	36.5
2	39.0
3	106.0
4	173.0
5	173.0
6	228.0
7	283.0
8	330.0
9	377.0
10	614.0
11	851.0
12	851.0
13	1732.0
14	2613.0
15	5046.0
16	7479.0
17	7248.5
18	7018.0
19	7018.0
20	7828.5
21	8639.0
22	7093.5
23	5548.0
24	5461.5
25	5375.0
26	5375.0
27	5664.0
28	5953.0
29	7263.5
30	8574.0
31	10151.0
32	11728.0
33	11728.0
34	14220.0
35	16712.0
36	18296.5
37	19881.0
38	23799.0
39	27717.0
40	27717.0
41	33191.5
42	38666.0
43	45046.5
44	51427.0
45	65799.5
46	80172.0
47	80172.0
48	88636.0
49	97100.0
50	105180.5
51	113261.0
52	110881.0
53	108501.0
54	108501.0
55	96382.5
56	84264.0
57	76939.0
58	69614.0
59	63276.0
60	56938.0
61	56938.0
62	50829.0
63	44720.0
64	35724.5
65	26729.0
66	23057.0
67	19385.0
68	19385.0
69	16536.0
70	13687.0
71	11253.0
72	8819.0
73	7062.5
74	5306.0
75	5306.0
76	4218.5
77	3131.0
78	2465.0
79	1799.0
80	1411.5
81	1024.0
82	1024.0
83	796.0
84	568.0
85	449.5
86	331.0
87	251.5
88	172.0
89	172.0
90	140.0
91	108.0
92	63.5
93	19.0
94	11.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	954739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.028083504141605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.68257139620562	33.04998467796547
2	6.7827035083195915	5.294318378130624
3	2.4029434744584317	2.813468357306875
4	1.2180062909651326	1.90145804929428
5	0.7886308497793872	1.538937532956604
6	0.5957657025260114	1.3950956152253258
7	0.40959427321044206	1.1189975648372725
8	0.32415204953563637	1.012082660585237
9	0.262387658560811	0.9216438703890775
>10	2.02029778217158	15.748449343456702
>50	0.2809189353932727	7.6862044631559785
>100	0.20150517676380558	15.784910796734577
>500	0.019988449169572767	5.3210940304716585
>1k	0.010534452940720782	6.413354659490318
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4143	0.433940584809042	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3852	0.4034610506117379	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3123	0.32710510411746035	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2639	0.27641062112263143	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2288	0.239646646884646	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2136	0.2237260654482534	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1913	0.20036889663038798	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1731	0.18130609517365479	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1658	0.17366002645749257	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1653	0.17313632312076913	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1566	0.16402388506178128	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1498	0.1569015196823425	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1462	0.15313085565793375	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1426	0.14936019163352496	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1411	0.14778908162335466	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1384	0.14496108360504809	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1364	0.14286627025815432	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1364	0.14286627025815432	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1322	0.13846716222967742	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1288	0.13490597953995806	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1272	0.13323012886244304	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1264	0.13239220352368553	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	1234	0.1292499835033449	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1230	0.12883102083396614	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1176	0.123175024797353	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1166	0.12212761812390611	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	1158	0.12128969278514862	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	1146	0.12003280477701236	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1141	0.11950910144028892	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1140	0.11940436077294424	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1123	0.11762376942808454	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1120	0.11730954742605046	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	1116	0.11689058475667172	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	1083	0.11343414273429701	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	1073	0.11238673606085013	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1064	0.11144407005474793	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	1051	0.11008244137926701	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	1047	0.10966347870988825	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	1011	0.10589281468547948	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	973	0.10191266932638135	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	965	0.10107474398762385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.0474066734468792E-4	0.0	0.0	0.0	0.0
9	1.0474066734468792E-4	0.0	0.0	0.0	0.0
10	1.0474066734468792E-4	0.0	0.0	0.0	0.0
11	1.0474066734468792E-4	0.0	0.0	0.0	0.0
12	1.0474066734468792E-4	0.0	0.0	0.0	0.0
13	1.0474066734468792E-4	0.0	0.0	0.0	0.0
14	2.0948133468937585E-4	0.0	0.0	1.0474066734468792E-4	0.0
15	3.1422200203406374E-4	0.0	0.0	3.1422200203406374E-4	0.0
16	3.1422200203406374E-4	0.0	0.0	0.001361628675480943	0.0
17	3.1422200203406374E-4	0.0	0.0	0.0019900726795490705	0.0
18	4.189626693787517E-4	0.0	0.0	0.0023042946815831343	0.0
19	4.189626693787517E-4	0.0	0.0	0.0028279980183065737	0.0
20	4.189626693787517E-4	0.0	0.0	0.003561182689719389	0.0
21	4.189626693787517E-4	0.0	0.0	0.006703402710060027	0.0
22	4.189626693787517E-4	0.0	0.0	0.008274512720230345	0.0
23	4.189626693787517E-4	0.0	0.0	0.010264585399779416	0.0
24	4.189626693787517E-4	0.0	0.0	0.013197324085430678	0.0
25	4.189626693787517E-4	0.0	0.0	0.015396878099669125	0.0
26	5.237033367234396E-4	0.0	0.0	0.019795986128146016	0.0
27	5.237033367234396E-4	0.0	0.0	0.04357211761539018	0.0
28	5.237033367234396E-4	0.0	0.0	0.1551209283374828	0.0
29	5.237033367234396E-4	0.0	0.0	0.3347511728336226	0.0
30	5.237033367234396E-4	0.0	0.0	0.5558587215982588	0.0
31	5.237033367234396E-4	0.0	0.0	1.192472497719272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1995	0.0	30.323307	1
ATACACA	195	0.0	23.717949	37
AAGGGCG	40	0.0019309493	23.125	5
ATACGGC	105	4.0017767E-11	22.90476	29
GATGCTA	70	5.100428E-6	21.142859	14
AACGACC	105	9.822543E-10	21.142857	37
CGGAATG	45	0.003825341	20.555555	14
TTACTCG	45	0.003825341	20.555555	29
TCGGACT	45	0.003825341	20.555555	33
TTATACT	55	5.1422167E-4	20.181818	4
TAAACCG	65	6.900564E-5	19.923077	5
GTTATCG	65	6.900564E-5	19.923077	11
GTATCAA	3010	0.0	19.913622	2
CTTAAAC	85	1.2448563E-6	19.588234	3
TTTTACG	85	1.2448563E-6	19.588234	4
CAGTACT	60	9.2352525E-4	18.5	4
AACAGTC	50	0.0070340307	18.5	7
ACCCATC	50	0.0070340307	18.5	8
TACCGCT	70	1.21892255E-4	18.5	35
TATCCGG	60	9.2352525E-4	18.5	10
>>END_MODULE