FastQCFastQC Report
Fri 10 Feb 2017
ERR1631938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42103
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1660.39427119207657413No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1380.32776761750944117No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1330.3158919791938817No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1200.2850153195734271No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1130.26838942593164383No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1060.2517635322898606No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT980.23276251098496545No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA930.22088687266940596No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT920.21851174500629408No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA870.20663610669073465No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC850.20188585136451084No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT820.1947604683751752No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT800.19001021304895138No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG760.18050970239650382No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT750.17813457473339192No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC700.16625893641783246No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA680.16150868109160868No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA650.154383298102273No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC640.1520081704391611No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA620.14725791511293732No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA570.13538227679737785No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG560.13300714913426598No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT560.13300714913426598No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC550.13063202147115408No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT530.1258817661449303No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC520.12350663848181839No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA520.12350663848181839No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC510.12113151081870652No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA510.12113151081870652No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC510.12113151081870652No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA490.11638125549248272No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC490.11638125549248272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT480.11400612782937083No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC480.11400612782937083No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC460.10925587250314704No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC450.10688074484003515No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA450.10688074484003515No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT450.10688074484003515No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG450.10688074484003515No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA440.10450561717692326No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG440.10450561717692326No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC430.10213048951381136No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG430.10213048951381136No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA430.10213048951381136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGAG251.21354016E-437.033
TCAATGA251.21354016E-437.032
TGAGCAA251.21354016E-437.036
AATGAGC251.21354016E-437.034
GTCTGTC352.3345063E-531.71428735
GTCAATG303.541422E-430.83333231
TAACGTC303.541422E-430.83333227
GAGCAAA303.541422E-430.83333237
CGGGTAA303.541422E-430.83333223
TGCGGGT303.541422E-430.83333221
ATTAGCA250.0054371529.629
TAGCACG250.0054371529.631
ACCGACG250.0054371529.612
GCACGTC250.0054371529.633
TACCGAC250.0054371529.611
CAGATTA250.0054371529.626
AGCACGT250.0054371529.632
CGACGCT250.0054371529.614
GATTAGC250.0054371529.628
CGGCACC250.0054371529.64
Produced by FastQC (version 0.11.5)