##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631938.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42103
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50224449564164	34.0	31.0	34.0	31.0	34.0
2	32.68353799016697	34.0	31.0	34.0	31.0	34.0
3	32.78343585967746	34.0	31.0	34.0	31.0	34.0
4	36.2198893190509	37.0	37.0	37.0	35.0	37.0
5	36.14561907702539	37.0	35.0	37.0	35.0	37.0
6	36.187777593045624	37.0	36.0	37.0	35.0	37.0
7	36.180794717716076	37.0	36.0	37.0	35.0	37.0
8	36.160962401729094	37.0	36.0	37.0	35.0	37.0
9	38.00102130489514	39.0	38.0	39.0	35.0	39.0
10	37.928176139467496	39.0	38.0	39.0	35.0	39.0
11	37.99729235446405	39.0	38.0	39.0	35.0	39.0
12	37.915041683490486	39.0	38.0	39.0	35.0	39.0
13	37.98066646082227	39.0	38.0	39.0	35.0	39.0
14	39.35436904733629	40.0	39.0	41.0	36.0	41.0
15	39.34586609030235	40.0	39.0	41.0	36.0	41.0
16	39.29824478065696	40.0	39.0	41.0	36.0	41.0
17	39.28190390233475	40.0	39.0	41.0	36.0	41.0
18	39.301474954278795	40.0	39.0	41.0	36.0	41.0
19	39.29693846044225	40.0	39.0	41.0	36.0	41.0
20	39.29769850129444	40.0	39.0	41.0	36.0	41.0
21	39.27891124147923	40.0	39.0	41.0	36.0	41.0
22	39.25159727335344	40.0	39.0	41.0	36.0	41.0
23	39.19269410730827	40.0	39.0	41.0	36.0	41.0
24	39.19825190603995	40.0	39.0	41.0	36.0	41.0
25	39.130038239555375	40.0	39.0	41.0	36.0	41.0
26	39.07818920266964	40.0	39.0	41.0	36.0	41.0
27	38.93337766904971	40.0	38.0	41.0	35.0	41.0
28	38.937842909056364	40.0	38.0	41.0	35.0	41.0
29	38.94470702800275	40.0	38.0	41.0	35.0	41.0
30	38.896753200484525	40.0	38.0	41.0	35.0	41.0
31	38.78982495309123	40.0	38.0	41.0	35.0	41.0
32	38.772961546683135	40.0	38.0	41.0	35.0	41.0
33	38.68824074293993	40.0	38.0	41.0	35.0	41.0
34	38.67358620525853	40.0	38.0	41.0	35.0	41.0
35	38.5905992447094	40.0	38.0	41.0	35.0	41.0
36	38.55651616274375	40.0	38.0	41.0	35.0	41.0
37	38.485191079020495	40.0	38.0	41.0	35.0	41.0
38	38.46379117877586	40.0	38.0	41.0	34.0	41.0
39	38.37187373821343	40.0	38.0	41.0	34.0	41.0
40	38.0718951143624	40.0	37.0	41.0	34.0	41.0
41	38.14920551979669	40.0	37.0	41.0	34.0	41.0
42	38.13941999382467	40.0	37.0	41.0	34.0	41.0
43	37.66016673396195	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	3.0
22	6.0
23	3.0
24	19.0
25	38.0
26	61.0
27	102.0
28	132.0
29	225.0
30	282.0
31	432.0
32	543.0
33	821.0
34	1112.0
35	1716.0
36	2690.0
37	4648.0
38	10599.0
39	18668.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.19414293518277	16.433508301071182	13.12970572168254	28.24264304206351
2	21.535282521435526	19.822815476331854	31.270930812531173	27.37097118970145
3	21.29776975512434	19.048523858157377	29.686720661235544	29.966985725482743
4	15.307697788756144	16.357504215851602	35.425029095313874	32.909768900078376
5	16.250623471011565	33.446547751941665	33.53917773080303	16.763651046243737
6	32.82426430420635	34.638861838823836	15.863477661924327	16.673396195045484
7	29.320951001116306	27.55860627508729	20.340593306890245	22.77984941690616
8	26.646557252452318	30.023988789397432	21.483029712847067	21.846424245303183
9	28.553784765931166	12.345913592855617	17.25055221718167	41.84974942403154
10	19.27891124147923	23.35938056670546	29.14756668170914	28.214141510106167
11	39.74063605918818	18.894140560055103	18.806260836519964	22.558962544236753
12	22.138564947865948	23.782153290739377	27.188086359641833	26.891195401752842
13	36.10431560696387	17.238676578866112	20.59473196684322	26.062275847326795
14	23.75840201410826	20.076954136284826	22.950858608650215	33.2137852409567
15	30.121369023585014	24.079044248628364	19.64468090159846	26.154905826188156
16	24.611072845165427	24.575445930218752	22.805975821200388	28.007505403415433
17	27.437474764268575	23.689523311878013	21.217015414578533	27.65598650927487
18	26.855568486806163	20.927249839678883	23.281001353822766	28.936180319692184
19	27.817495190366483	22.26207158634777	23.539890269101964	26.380542954183788
20	31.01204189725198	19.898819561551434	22.653967650761228	26.435170890435362
21	27.27596608317697	21.760919649431155	21.608911478991995	29.354202788399874
22	28.563285276583617	22.145690330855285	21.20276464859986	28.088259743961235
23	28.49203144669026	21.658789159917347	22.162316224497065	27.686863168895325
24	28.13813742488659	21.174263116642518	22.960359119302662	27.72724033916823
25	28.49440657435337	21.720542479158254	23.178870864308955	26.606180082179414
26	28.42315274446002	22.75372301261193	21.958055245469442	26.865068997458614
27	26.796190295228367	20.93912547799444	24.839085100824168	27.42559912595302
28	26.48742369902382	23.01023680022801	21.573284564045316	28.929054936702848
29	27.064579721160015	24.06004322732347	21.99368216041612	26.881694891100395
30	27.133458423390255	21.52815713844619	23.979288886777663	27.359095551385888
31	28.387525829513336	22.513835118637626	22.917606821366647	26.181032230482387
32	26.27128708168064	21.72766786214759	22.611215352825216	29.389829703346553
33	26.430420635109137	21.578034819371542	24.409186993800915	27.582357551718406
34	26.416169869130467	21.939054224164547	24.041042206018574	27.603733700686412
35	25.48036956986438	23.739400992803365	24.732204355984134	26.048025081348126
36	26.325915017932218	22.212193905422417	25.04334607985179	26.41854499679358
37	27.064579721160015	21.908177564544093	24.430563142768925	26.59667957152697
38	25.501745718832385	21.770420160083603	25.632377740303543	27.09545638078047
39	25.13835118637627	20.114956178894616	26.34491603923711	28.401776595492006
40	24.480440823694273	21.57565969170843	27.98850438211054	25.95539510248676
41	23.114742417404933	20.542479158254757	28.223642020758614	28.11913640358169
42	21.63028762796	21.08638339310738	29.501460703512816	27.781868275419804
43	20.50922737097119	21.95092986248011	29.05731183051089	28.482530936037815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	2.0
6	2.5
7	3.0
8	1.5
9	0.0
10	5.0
11	10.0
12	10.0
13	26.0
14	42.0
15	67.0
16	92.0
17	77.5
18	63.0
19	63.0
20	75.5
21	88.0
22	79.0
23	70.0
24	76.5
25	83.0
26	83.0
27	83.5
28	84.0
29	131.5
30	179.0
31	209.5
32	240.0
33	240.0
34	325.0
35	410.0
36	448.5
37	487.0
38	658.5
39	830.0
40	830.0
41	1005.0
42	1180.0
43	1372.0
44	1564.0
45	2383.5
46	3203.0
47	3203.0
48	3640.0
49	4077.0
50	4643.0
51	5209.0
52	5300.5
53	5392.0
54	5392.0
55	4761.5
56	4131.0
57	3949.5
58	3768.0
59	3499.0
60	3230.0
61	3230.0
62	2951.0
63	2672.0
64	2152.0
65	1632.0
66	1398.0
67	1164.0
68	1164.0
69	994.5
70	825.0
71	698.5
72	572.0
73	455.5
74	339.0
75	339.0
76	262.5
77	186.0
78	147.5
79	109.0
80	97.5
81	86.0
82	86.0
83	65.5
84	45.0
85	31.5
86	18.0
87	12.5
88	7.0
89	7.0
90	6.0
91	5.0
92	4.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	42103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.66292188205116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.18862856290106	54.87019927321094
2	6.532607073571109	8.317697076217847
3	2.421280405909566	4.624373560078855
4	1.2647366064766452	3.220673111179726
5	0.8431577376510969	2.683894259316438
6	0.540964035218624	2.066361066907346
7	0.39173257722727955	1.7457188323872406
8	0.3059244888822564	1.5580837470014015
9	0.1865393224891807	1.0688074484003516
>10	1.2125055961796747	14.127259340189536
>50	0.08953887479480674	3.8738332185354962
>100	0.022384718698701685	1.8430990665748284
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	166	0.39427119207657413	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	138	0.32776761750944117	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	133	0.3158919791938817	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	120	0.2850153195734271	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	113	0.26838942593164383	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	106	0.2517635322898606	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	98	0.23276251098496545	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	93	0.22088687266940596	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	92	0.21851174500629408	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	87	0.20663610669073465	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	85	0.20188585136451084	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	82	0.1947604683751752	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	80	0.19001021304895138	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	76	0.18050970239650382	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	75	0.17813457473339192	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	70	0.16625893641783246	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	68	0.16150868109160868	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	65	0.154383298102273	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	64	0.1520081704391611	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	62	0.14725791511293732	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	57	0.13538227679737785	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	56	0.13300714913426598	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	56	0.13300714913426598	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	55	0.13063202147115408	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	53	0.1258817661449303	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	52	0.12350663848181839	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	52	0.12350663848181839	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	51	0.12113151081870652	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	51	0.12113151081870652	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	51	0.12113151081870652	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	49	0.11638125549248272	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	49	0.11638125549248272	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.11400612782937083	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	48	0.11400612782937083	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	46	0.10925587250314704	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	45	0.10688074484003515	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	45	0.10688074484003515	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	45	0.10688074484003515	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	45	0.10688074484003515	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	44	0.10450561717692326	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	44	0.10450561717692326	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	43	0.10213048951381136	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	43	0.10213048951381136	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	43	0.10213048951381136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.002375127663111892	0.0
17	0.0	0.0	0.0	0.004750255326223784	0.0
18	0.0	0.0	0.0	0.004750255326223784	0.0
19	0.0	0.0	0.0	0.004750255326223784	0.0
20	0.0	0.0	0.0	0.004750255326223784	0.0
21	0.0	0.0	0.0	0.004750255326223784	0.0
22	0.0	0.0	0.0	0.009500510652447568	0.0
23	0.0	0.0	0.0	0.009500510652447568	0.0
24	0.0	0.0	0.0	0.009500510652447568	0.0
25	0.0	0.0	0.0	0.011875638315559461	0.0
26	0.0	0.0	0.0	0.011875638315559461	0.0
27	0.0	0.0	0.0	0.02850153195734271	0.0
28	0.0	0.0	0.0	0.11875638315559461	0.0
29	0.0	0.0	0.0	0.2778899365840914	0.0
30	0.0	0.0	0.0	0.47977578794860226	0.0
31	0.0	0.0	0.0	0.969052086549652	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGAG	25	1.21354016E-4	37.0	33
TCAATGA	25	1.21354016E-4	37.0	32
TGAGCAA	25	1.21354016E-4	37.0	36
AATGAGC	25	1.21354016E-4	37.0	34
GTCTGTC	35	2.3345063E-5	31.714287	35
GTCAATG	30	3.541422E-4	30.833332	31
TAACGTC	30	3.541422E-4	30.833332	27
GAGCAAA	30	3.541422E-4	30.833332	37
CGGGTAA	30	3.541422E-4	30.833332	23
TGCGGGT	30	3.541422E-4	30.833332	21
ATTAGCA	25	0.00543715	29.6	29
TAGCACG	25	0.00543715	29.6	31
ACCGACG	25	0.00543715	29.6	12
GCACGTC	25	0.00543715	29.6	33
TACCGAC	25	0.00543715	29.6	11
CAGATTA	25	0.00543715	29.6	26
AGCACGT	25	0.00543715	29.6	32
CGACGCT	25	0.00543715	29.6	14
GATTAGC	25	0.00543715	29.6	28
CGGCACC	25	0.00543715	29.6	4
>>END_MODULE