FastQCFastQC Report
Fri 10 Feb 2017
ERR1631937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690167
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19780.2865973018124599No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA19140.27732418385695057No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18520.26834085083755094No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17130.2482007977779291No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC14230.2061819820420275No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA14110.20444327242536953No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13640.19763332642679238No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12900.1869112837907347No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT12750.1847378967699122No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12500.1811155850685414No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12090.17517499387829322No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12050.1745954240060739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11520.16691612319916774No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11150.16155510188113892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10750.1557594031589456No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10100.14634139273538144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9070.13141746852573363No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9050.13112768358962398No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8510.12330349031466298No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8210.11895671627301797No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7810.11316101755082465No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7790.11287123261471499No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7690.11142230793416666No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7670.111132522998057No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7580.1098284907855635No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7440.10779999623279582No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7320.10606128661613784No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG7160.1037430071272605No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG7130.103308329723096No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7110.10301854478698634No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7030.10185940504254767No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7010.10156962010643801No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT7000.10142472763838316No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6910.10012069542588967No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA251.2331147E-436.99999619
ATACGGC1500.032.06666629
ATTACGT303.5980024E-430.83333217
ACGCTAA250.005494841429.59999834
GGTATCA11200.028.5758931
TACGGCT1800.026.72222130
TTTAACA358.865789E-426.4285722
TTACGTT358.865789E-426.42857218
AATTACG400.001930409623.12516
CAATACA652.6802663E-622.769234
GGAGTGT603.723511E-521.5833326
TCAAGAT705.0970484E-621.1428593
ATACACA1400.021.14285937
GCGGTAA7700.020.6623423
TACACAG450.003824282720.5555555
CGCGGTA7750.020.29032122
TGTGACA555.1402336E-420.18181810
TGCGGGT4700.020.07446721
TTCTGCG4950.019.8080818
CCGCGGT8050.019.76397521
Produced by FastQC (version 0.11.5)