##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631937.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690167
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.401137695659166	33.0	31.0	34.0	31.0	34.0
2	32.57084589671775	34.0	31.0	34.0	31.0	34.0
3	32.65916944739462	34.0	31.0	34.0	31.0	34.0
4	36.148721975985524	37.0	35.0	37.0	35.0	37.0
5	36.04811878864101	37.0	35.0	37.0	35.0	37.0
6	36.09817044280587	37.0	35.0	37.0	35.0	37.0
7	36.097603913255774	37.0	35.0	37.0	35.0	37.0
8	36.075772965093954	37.0	35.0	37.0	35.0	37.0
9	37.88399474330126	39.0	38.0	39.0	35.0	39.0
10	37.78468689462116	39.0	37.0	39.0	35.0	39.0
11	37.875483759727715	39.0	38.0	39.0	35.0	39.0
12	37.78942198047719	39.0	38.0	39.0	35.0	39.0
13	37.86500078966395	39.0	38.0	39.0	35.0	39.0
14	39.17460991325288	40.0	39.0	41.0	36.0	41.0
15	39.181305394201694	40.0	39.0	41.0	36.0	41.0
16	39.118304700166775	40.0	38.0	41.0	36.0	41.0
17	39.10114218732568	40.0	38.0	41.0	36.0	41.0
18	39.13131169702405	40.0	39.0	41.0	36.0	41.0
19	39.14689343303867	40.0	39.0	41.0	36.0	41.0
20	39.14020664563794	40.0	39.0	41.0	36.0	41.0
21	39.09974687285831	40.0	39.0	41.0	36.0	41.0
22	39.07406758074495	40.0	39.0	41.0	36.0	41.0
23	39.00747065565291	40.0	39.0	41.0	36.0	41.0
24	39.02224389169578	40.0	38.0	41.0	36.0	41.0
25	38.96248154432188	40.0	38.0	41.0	35.0	41.0
26	38.89264482364413	40.0	38.0	41.0	35.0	41.0
27	38.758554089082786	40.0	38.0	41.0	35.0	41.0
28	38.76964560751239	40.0	38.0	41.0	35.0	41.0
29	38.73977312737352	40.0	38.0	41.0	35.0	41.0
30	38.696515481035746	40.0	38.0	41.0	35.0	41.0
31	38.60489417778596	40.0	38.0	41.0	35.0	41.0
32	38.555271405326536	40.0	38.0	41.0	35.0	41.0
33	38.48890630818338	40.0	38.0	41.0	34.0	41.0
34	38.47547332747002	40.0	38.0	41.0	34.0	41.0
35	38.389499932625	40.0	38.0	41.0	34.0	41.0
36	38.34080881873518	40.0	38.0	41.0	34.0	41.0
37	38.29946520190041	40.0	38.0	41.0	34.0	41.0
38	38.2588996576191	40.0	38.0	41.0	34.0	41.0
39	38.17581107181305	40.0	37.0	41.0	34.0	41.0
40	37.886711477077284	40.0	37.0	41.0	33.0	41.0
41	37.94659553412435	40.0	37.0	41.0	33.0	41.0
42	37.92656125256641	40.0	37.0	41.0	33.0	41.0
43	37.43392541225529	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	2.0
18	0.0
19	13.0
20	23.0
21	51.0
22	126.0
23	267.0
24	449.0
25	721.0
26	1188.0
27	1833.0
28	2758.0
29	3954.0
30	5705.0
31	7756.0
32	10470.0
33	14033.0
34	20409.0
35	30239.0
36	46525.0
37	84279.0
38	192709.0
39	266654.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.82586678296702	16.604242161679707	12.853121056208137	27.716769999145136
2	20.508949283289407	19.940536131110296	32.74801605988116	26.802498525719137
3	21.14633125026262	19.987046613355897	29.81698632359994	29.049635812781542
4	15.065049473533218	16.431095662354185	35.12758506274568	33.37626980136692
5	16.26823652826055	33.35468082362675	33.9768490814542	16.400233566658503
6	32.69513030904114	34.66392916497022	16.2337521208635	16.407188405125137
7	29.589070471349686	28.388926158451504	20.40622052343853	21.615782846760276
8	27.02896545328884	30.693585755331682	20.813223466204555	21.464225325174922
9	28.48136755307049	12.829648476383252	18.00578700517411	40.68319696537215
10	19.014093690367694	23.7015099244096	29.890157019967635	27.394239365255075
11	38.735407517311025	19.791876458886037	19.296634003074615	22.176082020728316
12	22.12493497950496	24.307015548410746	27.319474851738786	26.24857462034551
13	35.07527888177789	17.86364749401232	21.201969957995672	25.859103666214118
14	23.47460831943573	20.59545008671814	23.985644054265126	31.944297539581
15	29.322178545192685	24.544059626148453	20.55792293749194	25.575838891166917
16	24.497404251434798	24.82602036898316	23.292188702154697	27.384386677427347
17	26.39868321725032	24.280355334288657	22.18492046127966	27.136040987181364
18	26.322469779053474	21.624621287311623	23.993758032476197	28.059150901158702
19	27.489143931830988	22.973135487497952	24.10648437262286	25.431236208048198
20	29.687887134563084	21.09692291865592	23.27016504701036	25.945024899770637
21	26.91884717756717	22.686248400749385	22.588301092344317	27.80660332933913
22	28.00901810721173	23.120636019977773	22.16304169860338	26.707304174207113
23	27.532756564715495	22.349083627585784	23.14063118056934	26.977528627129377
24	27.25195496162523	21.923969126312905	23.831478468254787	26.992597443807075
25	27.522758984419717	22.283882016961112	23.83104379085062	26.362315207768557
26	27.404671622955025	23.06775026913776	23.178737899667762	26.348840208239455
27	26.301605263653578	22.167533365113083	25.038722512087652	26.492138859145687
28	25.862870870383546	23.397380633962506	22.83925484701529	27.900493648638662
29	26.651230789069892	24.09851528687984	22.970672315541023	26.279581608509243
30	26.408970582482212	22.505422600616953	24.36598678290906	26.719620033991774
31	27.474799577493563	22.86026425488324	23.435777137997036	26.229159029626164
32	25.189845356268847	22.77637151587949	23.757728202014874	28.276054925836792
33	25.289966051694734	22.295763199341607	25.39776604792753	27.016504701036126
34	25.98110312431629	22.450943032628334	24.507256939262525	27.060696903792852
35	25.048864984851495	24.03490749340377	25.154201809127358	25.76202571261738
36	25.655674641065136	22.713633077211746	25.558596687468395	26.072095594254723
37	26.426647463584903	22.43543953854647	25.002644287542005	26.13526871032663
38	25.342996695002807	21.94541321158502	25.848671408514175	26.862918684898002
39	24.948744289425605	20.859009486109883	26.916673790546348	27.275572433918168
40	24.88571606582175	21.66953795240862	27.608245540572064	25.836500441197565
41	23.01080752919221	21.267606246024513	28.457025618437275	27.264560606346
42	21.635488222415734	21.565939837749415	29.443743325890694	27.35482861394416
43	20.900738516909676	22.44630647365058	28.93560544042239	27.71734956901735
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	4.5
2	7.0
3	26.5
4	46.0
5	46.0
6	65.0
7	84.0
8	94.0
9	104.0
10	147.0
11	190.0
12	190.0
13	423.5
14	657.0
15	1309.5
16	1962.0
17	1929.5
18	1897.0
19	1897.0
20	2154.0
21	2411.0
22	2018.0
23	1625.0
24	1776.5
25	1928.0
26	1928.0
27	2247.0
28	2566.0
29	3382.5
30	4199.0
31	4954.0
32	5709.0
33	5709.0
34	7213.5
35	8718.0
36	9798.0
37	10878.0
38	14053.5
39	17229.0
40	17229.0
41	21067.0
42	24905.0
43	28542.0
44	32179.0
45	44369.0
46	56559.0
47	56559.0
48	62545.0
49	68531.0
50	76131.0
51	83731.0
52	84171.0
53	84611.0
54	84611.0
55	74602.0
56	64593.0
57	59817.5
58	55042.0
59	51124.5
60	47207.0
61	47207.0
62	42814.5
63	38422.0
64	31127.0
65	23832.0
66	20550.5
67	17269.0
68	17269.0
69	14675.5
70	12082.0
71	10189.5
72	8297.0
73	6646.5
74	4996.0
75	4996.0
76	4087.0
77	3178.0
78	2591.5
79	2005.0
80	1566.5
81	1128.0
82	1128.0
83	908.5
84	689.0
85	524.0
86	359.0
87	274.0
88	189.0
89	189.0
90	146.5
91	104.0
92	70.5
93	37.0
94	23.0
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	690167.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.30965884842457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.64888009268982	40.99328956783202
2	6.40610535840124	6.061413181060545
3	2.055215149348305	2.9169458272734676
4	1.0519274509872028	1.9906531535798966
5	0.6482945127744127	1.5335296116331545
6	0.42507441881393626	1.2066075443567803
7	0.34566898444026467	1.1447437209845213
8	0.265840504313208	1.0061458853720808
9	0.21554675964680567	0.9177699298296368
>10	1.5764050815071051	15.132673097742552
>50	0.19914234898379526	6.490106025480646
>100	0.14220055931443737	12.762515741330963
>500	0.014774084084616808	4.605688976924594
>1k	0.004924694694872269	3.237917736599174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1978	0.2865973018124599	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1914	0.27732418385695057	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1852	0.26834085083755094	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1713	0.2482007977779291	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1423	0.2061819820420275	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1411	0.20444327242536953	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1364	0.19763332642679238	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1290	0.1869112837907347	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1275	0.1847378967699122	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1250	0.1811155850685414	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1209	0.17517499387829322	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1205	0.1745954240060739	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.16691612319916774	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1115	0.16155510188113892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1075	0.1557594031589456	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1010	0.14634139273538144	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	907	0.13141746852573363	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	905	0.13112768358962398	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	851	0.12330349031466298	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	821	0.11895671627301797	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	781	0.11316101755082465	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	779	0.11287123261471499	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	769	0.11142230793416666	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	767	0.111132522998057	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	758	0.1098284907855635	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	744	0.10779999623279582	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	732	0.10606128661613784	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	716	0.1037430071272605	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	713	0.103308329723096	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	711	0.10301854478698634	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	703	0.10185940504254767	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	701	0.10156962010643801	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	700	0.10142472763838316	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	691	0.10012069542588967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.448924680548331E-4	0.0
4	0.0	0.0	0.0	1.448924680548331E-4	0.0
5	0.0	0.0	0.0	1.448924680548331E-4	0.0
6	0.0	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
7	0.0	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
8	0.0	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
9	0.0	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
10	1.448924680548331E-4	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
11	1.448924680548331E-4	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
12	1.448924680548331E-4	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
13	1.448924680548331E-4	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
14	1.448924680548331E-4	1.448924680548331E-4	0.0	1.448924680548331E-4	0.0
15	2.897849361096662E-4	1.448924680548331E-4	0.0	2.897849361096662E-4	0.0
16	2.897849361096662E-4	1.448924680548331E-4	0.0	5.795698722193324E-4	0.0
17	2.897849361096662E-4	1.448924680548331E-4	0.0	0.0010142472763838316	0.0
18	2.897849361096662E-4	1.448924680548331E-4	0.0	0.001448924680548331	0.0
19	2.897849361096662E-4	1.448924680548331E-4	0.0	0.0021733870208224967	0.0
20	4.346774041644993E-4	1.448924680548331E-4	0.0	0.002463171956932163	0.0
21	4.346774041644993E-4	1.448924680548331E-4	0.0	0.004491666509699827	0.0
22	5.795698722193324E-4	1.448924680548331E-4	0.0	0.006954838466631989	0.0
23	5.795698722193324E-4	1.448924680548331E-4	0.0	0.008258870679125487	0.0
24	5.795698722193324E-4	1.448924680548331E-4	0.0	0.009128225487454485	0.0
25	5.795698722193324E-4	1.448924680548331E-4	0.0	0.010866935104112484	0.0
26	5.795698722193324E-4	1.448924680548331E-4	0.0	0.013619891997154313	0.0
27	7.244623402741656E-4	1.448924680548331E-4	0.0	0.030572310759569784	0.0
28	7.244623402741656E-4	1.448924680548331E-4	0.0	0.10664085648835717	0.0
29	7.244623402741656E-4	1.448924680548331E-4	0.0	0.24298466892795512	0.0
30	7.244623402741656E-4	1.448924680548331E-4	0.0	0.4204779422951257	0.0
31	7.244623402741656E-4	1.448924680548331E-4	0.0	0.9394827628675378	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTTA	25	1.2331147E-4	36.999996	19
ATACGGC	150	0.0	32.066666	29
ATTACGT	30	3.5980024E-4	30.833332	17
ACGCTAA	25	0.0054948414	29.599998	34
GGTATCA	1120	0.0	28.575893	1
TACGGCT	180	0.0	26.722221	30
TTTAACA	35	8.865789E-4	26.428572	2
TTACGTT	35	8.865789E-4	26.428572	18
AATTACG	40	0.0019304096	23.125	16
CAATACA	65	2.6802663E-6	22.76923	4
GGAGTGT	60	3.723511E-5	21.583332	6
TCAAGAT	70	5.0970484E-6	21.142859	3
ATACACA	140	0.0	21.142859	37
GCGGTAA	770	0.0	20.66234	23
TACACAG	45	0.0038242827	20.555555	5
CGCGGTA	775	0.0	20.290321	22
TGTGACA	55	5.1402336E-4	20.181818	10
TGCGGGT	470	0.0	20.074467	21
TTCTGCG	495	0.0	19.80808	18
CCGCGGT	805	0.0	19.763975	21
>>END_MODULE