FastQCFastQC Report
Fri 10 Feb 2017
ERR1631936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62207
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2000.32150722587490155No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1770.2845338948992878No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1670.2684585336055428No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1660.26685099747616825No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1580.2539907084411722No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1460.23470027488867812No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1310.2105872329480605No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1300.20897969681868603No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1210.19451187165431544No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1200.1929043355249409No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1190.1912967993955664No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1090.17522143810182134No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT940.15110839616120372No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT850.13664057099683316No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC830.13342549873808413No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA830.13342549873808413No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT780.1253878180912116No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC720.11574260131496454No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA710.11413506518559004No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA710.11413506518559004No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT700.11252752905621553No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA690.11091999292684102No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC680.10931245679746651No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC670.10770492066809201No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC670.10770492066809201No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG670.10770492066809201No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT640.1028823122799685No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA630.10127477615059399No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC630.10127477615059399No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA630.10127477615059399No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA630.10127477615059399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG200.001828584837.018
GGTATCA1050.029.9523831
CAATGAG453.928726E-628.77777733
TCAATGA453.928726E-628.77777732
AATGAGC453.928726E-628.77777734
TCCGTGC1000.025.9000028
TGAGCAA508.911962E-625.90000236
GCGGTAA1051.8189894E-1224.66666823
AACTCCG1051.8189894E-1224.6666685
CGTGCCA1051.8189894E-1224.66666810
GTAATAC1051.8189894E-1224.66666826
CTCCGTG1051.8189894E-1224.6666687
GGTAATA1051.8189894E-1224.66666825
GTGCCAG1051.8189894E-1224.66666811
TAATACG901.3460522E-1024.66666627
ATACGGA851.886292E-923.94117529
GAGCAAA551.8654322E-523.54545437
TAACTCC1101.8189894E-1223.5454544
CTAACTC1101.8189894E-1223.5454543
CGCGGTA1101.8189894E-1223.54545422