##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631936.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62207
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56201070619062	34.0	31.0	34.0	31.0	34.0
2	32.72734579709679	34.0	31.0	34.0	31.0	34.0
3	32.82514829520793	34.0	31.0	34.0	31.0	34.0
4	36.23460382272091	37.0	37.0	37.0	35.0	37.0
5	36.16215216937	37.0	35.0	37.0	35.0	37.0
6	36.21333611972929	37.0	36.0	37.0	35.0	37.0
7	36.21412381243268	37.0	36.0	37.0	35.0	37.0
8	36.18716543154307	37.0	36.0	37.0	35.0	37.0
9	38.04427154500297	39.0	38.0	39.0	35.0	39.0
10	37.952159724789816	39.0	38.0	39.0	35.0	39.0
11	38.04322664651888	39.0	38.0	39.0	35.0	39.0
12	37.97545292330445	39.0	38.0	39.0	35.0	39.0
13	38.030141302425776	39.0	38.0	39.0	35.0	39.0
14	39.40292893082772	40.0	39.0	41.0	37.0	41.0
15	39.39558249071648	40.0	39.0	41.0	37.0	41.0
16	39.33112029192856	40.0	39.0	41.0	36.0	41.0
17	39.32153937659749	40.0	39.0	41.0	36.0	41.0
18	39.34700274888678	40.0	39.0	41.0	36.0	41.0
19	39.36497500281319	40.0	39.0	41.0	36.0	41.0
20	39.36122944363174	40.0	39.0	41.0	36.0	41.0
21	39.31956210715836	40.0	39.0	41.0	36.0	41.0
22	39.30427443856801	40.0	39.0	41.0	36.0	41.0
23	39.2187052904014	40.0	39.0	41.0	36.0	41.0
24	39.235070008198434	40.0	39.0	41.0	36.0	41.0
25	39.186056231613804	40.0	39.0	41.0	36.0	41.0
26	39.1120452682174	40.0	39.0	41.0	36.0	41.0
27	39.00980597038919	40.0	39.0	41.0	35.0	41.0
28	38.99901940296108	40.0	39.0	41.0	35.0	41.0
29	38.963573231308374	40.0	38.0	41.0	35.0	41.0
30	38.93277283906956	40.0	38.0	41.0	35.0	41.0
31	38.838426543636565	40.0	38.0	41.0	35.0	41.0
32	38.80507016895205	40.0	38.0	41.0	35.0	41.0
33	38.76214895429775	40.0	38.0	41.0	35.0	41.0
34	38.736589129840695	40.0	38.0	41.0	35.0	41.0
35	38.66265854325076	40.0	38.0	41.0	35.0	41.0
36	38.6093526452007	40.0	38.0	41.0	35.0	41.0
37	38.58617197421512	40.0	38.0	41.0	35.0	41.0
38	38.52074525374958	40.0	38.0	41.0	34.0	41.0
39	38.43014451749803	40.0	38.0	41.0	34.0	41.0
40	38.16967543845548	40.0	37.0	41.0	34.0	41.0
41	38.232369347501084	40.0	37.0	41.0	34.0	41.0
42	38.22630893629334	40.0	37.0	41.0	34.0	41.0
43	37.718520423746526	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	1.0
20	6.0
21	4.0
22	12.0
23	18.0
24	38.0
25	51.0
26	89.0
27	137.0
28	211.0
29	316.0
30	459.0
31	583.0
32	856.0
33	1097.0
34	1577.0
35	2431.0
36	3743.0
37	6419.0
38	15128.0
39	29030.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.356969472888906	16.113942160850065	13.120709887954732	28.4083784783063
2	21.090874017393542	19.71803816290771	31.920844920989598	27.270242898709146
3	20.936550548973585	19.780732071953317	29.31502885527352	29.967688523799573
4	15.487003070394007	16.070538685356954	34.746893436429986	33.69556480781905
5	16.366325333161864	32.88054399022618	34.12959956275017	16.623531113861784
6	33.14900252383172	34.54755895638755	15.832623338209526	16.470815181571204
7	29.81014998312087	27.628723455559662	20.153680453968203	22.407446107351262
8	27.339366952272254	30.478885012940665	20.803125050235504	21.37862298455158
9	28.450174417670038	12.712395711093608	17.311556577234075	41.52587329400228
10	19.226132107319113	23.43626923015095	29.477390004340347	27.860208658189595
11	39.55664153551851	19.03965791631167	18.55418200524057	22.849518542929253
12	22.421913932515633	24.069638465124505	26.572572218560612	26.935875383799253
13	36.02327712315334	17.380680630797176	20.761329110871767	25.83471313517771
14	23.95068079155079	20.18743871268507	22.852733615188	33.00914688057614
15	29.464529715305353	24.212709180638836	19.67141961515585	26.651341488899966
16	25.024514925972962	24.195026283215714	22.39619335444564	28.38426543636568
17	27.331329271625382	23.420193868857204	21.370585303904704	27.87789155561271
18	26.78958959602617	21.057115758676677	23.180670985580402	28.97262365971675
19	28.562701946726254	22.002347002748888	23.063320848136062	26.371630202388797
20	31.002941791116754	20.158503062356324	22.489430449949364	26.349124696577558
21	27.63515360007716	21.76121658334271	21.76443165560146	28.83919816097867
22	28.884209172601157	22.553731895124344	21.052293150288552	27.50976578198595
23	28.117414438889515	22.01038468339576	22.04896555050075	27.82323532721398
24	28.25566254601572	21.346472261964088	22.761104055813654	27.636761136206534
25	28.45821209831691	21.647081518157123	22.798077386789267	27.096628996736698
26	27.88592923625958	22.857556223576125	22.245084958284437	27.011429581879852
27	26.84263828829553	21.430064140691563	24.08571382641825	27.641583744594662
28	26.390920635941296	23.14530519073416	21.677624704615237	28.78614946870931
29	27.25256000128603	23.613098204382144	22.235439741508188	26.898902052823637
30	26.556496857266865	22.055395695018245	23.714372980532737	27.673734467182147
31	28.368190075071936	22.455672191232498	22.494253058337485	26.68188467535808
32	25.768804153873358	22.183998585368204	22.851126079058627	29.196071181699807
33	26.103171668783254	21.558667031041523	24.709437844615557	27.628723455559662
34	26.697960036651825	21.856061214975806	23.635603710193386	27.810375038178982
35	25.283328242802256	23.378397929493467	25.278505634414135	26.059768193290147
36	26.349124696577558	21.94608323822078	25.545356631890304	26.159435433311362
37	26.98570900380986	22.140595109875093	24.241644830967576	26.632051055347468
38	25.842750815824584	21.666371951709614	25.500345620267815	26.990531612197984
39	25.210989116980404	20.401241017891877	26.448791936598777	27.938977928528946
40	25.01326217306734	21.45417718263218	27.31686144646101	26.21569919783947
41	23.158165479769156	20.918867651550467	27.982381404022057	27.94058546465832
42	21.645473982027745	20.93012040445609	29.90981722314209	27.514588390374072
43	21.11980966772228	21.57634992846464	29.261980163004164	28.041860240808912
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0
7	4.0
8	4.0
9	4.0
10	5.0
11	6.0
12	6.0
13	19.5
14	33.0
15	69.5
16	106.0
17	96.5
18	87.0
19	87.0
20	94.0
21	101.0
22	92.0
23	83.0
24	100.0
25	117.0
26	117.0
27	132.5
28	148.0
29	217.5
30	287.0
31	341.5
32	396.0
33	396.0
34	531.5
35	667.0
36	764.5
37	862.0
38	1083.5
39	1305.0
40	1305.0
41	1581.5
42	1858.0
43	2163.5
44	2469.0
45	3529.0
46	4589.0
47	4589.0
48	5219.0
49	5849.0
50	6703.5
51	7558.0
52	7687.5
53	7817.0
54	7817.0
55	6972.5
56	6128.0
57	5845.5
58	5563.0
59	5144.0
60	4725.0
61	4725.0
62	4256.5
63	3788.0
64	3135.5
65	2483.0
66	2112.5
67	1742.0
68	1742.0
69	1482.0
70	1222.0
71	1067.0
72	912.0
73	712.5
74	513.0
75	513.0
76	407.0
77	301.0
78	251.0
79	201.0
80	164.0
81	127.0
82	127.0
83	98.5
84	70.0
85	60.0
86	50.0
87	34.5
88	19.0
89	19.0
90	16.5
91	14.0
92	8.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	62207.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.66336585914768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.46776634770785	54.18361277669715
2	5.972140273466561	7.484688218367708
3	2.4447807906415946	4.595945793881717
4	1.2262384238475155	3.0736090793640587
5	0.9568764269772453	2.998054881283457
6	0.56694286960314	2.131592907550597
7	0.4515020138015956	1.9804845113893934
8	0.2693619968702701	1.3503303486745866
9	0.2667966445191247	1.5046538170945392
>10	1.241630537954388	13.757294195187036
>50	0.10517944639696261	4.136190460880608
>100	0.03078422821374516	2.8035430096291414
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	200	0.32150722587490155	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	177	0.2845338948992878	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	167	0.2684585336055428	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	166	0.26685099747616825	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	158	0.2539907084411722	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	146	0.23470027488867812	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	131	0.2105872329480605	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	130	0.20897969681868603	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	121	0.19451187165431544	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	120	0.1929043355249409	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	119	0.1912967993955664	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	109	0.17522143810182134	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	94	0.15110839616120372	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	85	0.13664057099683316	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	83	0.13342549873808413	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	83	0.13342549873808413	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	78	0.1253878180912116	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	72	0.11574260131496454	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	71	0.11413506518559004	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	71	0.11413506518559004	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	70	0.11252752905621553	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	69	0.11091999292684102	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	68	0.10931245679746651	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	67	0.10770492066809201	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	67	0.10770492066809201	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	67	0.10770492066809201	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	64	0.1028823122799685	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	63	0.10127477615059399	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	63	0.10127477615059399	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	63	0.10127477615059399	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	63	0.10127477615059399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.004822608388123523	0.0
22	0.0	0.0	0.0	0.006430144517498031	0.0
23	0.0	0.0	0.0	0.006430144517498031	0.0
24	0.0	0.0	0.0	0.009645216776247046	0.0
25	0.0	0.0	0.0	0.012860289034996062	0.0
26	0.0	0.0	0.0	0.01446782516437057	0.0
27	0.0	0.0	0.0	0.02893565032874114	0.0
28	0.0	0.0	0.0	0.11895767357371356	0.0
29	0.0	0.0	0.0	0.2716736058642918	0.0
30	0.0	0.0	0.0	0.4597553330011092	0.0
31	0.0	0.0	0.0	1.0240005144115614	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGAAG	20	0.0018285848	37.0	18
GGTATCA	105	0.0	29.952383	1
CAATGAG	45	3.928726E-6	28.777777	33
TCAATGA	45	3.928726E-6	28.777777	32
AATGAGC	45	3.928726E-6	28.777777	34
TCCGTGC	100	0.0	25.900002	8
TGAGCAA	50	8.911962E-6	25.900002	36
GCGGTAA	105	1.8189894E-12	24.666668	23
AACTCCG	105	1.8189894E-12	24.666668	5
CGTGCCA	105	1.8189894E-12	24.666668	10
GTAATAC	105	1.8189894E-12	24.666668	26
CTCCGTG	105	1.8189894E-12	24.666668	7
GGTAATA	105	1.8189894E-12	24.666668	25
GTGCCAG	105	1.8189894E-12	24.666668	11
TAATACG	90	1.3460522E-10	24.666666	27
ATACGGA	85	1.886292E-9	23.941175	29
GAGCAAA	55	1.8654322E-5	23.545454	37
TAACTCC	110	1.8189894E-12	23.545454	4
CTAACTC	110	1.8189894E-12	23.545454	3
CGCGGTA	110	1.8189894E-12	23.545454	22
>>END_MODULE