FastQCFastQC Report
Fri 10 Feb 2017
ERR1631935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529003
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC34900.13799904547365108No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT26000.10280731181418132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.030.5961551
GTATCAA25100.018.9422322
GCGGTAA6650.018.639123
CTTATAC16700.018.05688737
TATCCGG2050.018.04878210
TAATACG6550.017.79389427
AAAACCG953.6098518E-617.5263165
TAACTCC8150.017.2515344
TGCGGGT4750.017.13684321
CGGTAAT7150.016.81818224
GCTAACT7950.016.7547192
GACAGGC12600.016.7380967
CCAGGAC15950.016.7021943
AACTCCG7900.016.6265815
CTAACTC8050.016.5465853
TGCATCA12300.016.54471614
TATACAC5400.016.44444337
TATTAGA8450.016.4201182
ATTAGAG8000.016.4187493
AGCCGCG8200.016.24390419
Produced by FastQC (version 0.11.5)