FastQCFastQC Report
Fri 10 Feb 2017
ERR1631934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266378
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8650.32472651645406153No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8110.3044545720742704No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8100.3040791656968668No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6570.2466419899541253No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6300.2365060177642298No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6120.22974870297096608No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5810.21811110527145636No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5250.19708834813685813No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4920.18469993768254137No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4840.18169668666331304No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4750.1783180292666812No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4650.1745639654926458No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4000.15016255096141573No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3930.14753470631959095No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3450.12951520020422108No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3450.12951520020422108No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3320.12463491729797506No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3320.12463491729797506No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3160.11862841525951842No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3140.11787760250471135No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3100.1163759769950972No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2960.11112028771144765No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2920.10961866220183349No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2900.1088678494470264No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2810.10548919205039455No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2790.10473837929558748No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2720.1021105346537627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT251.2310808E-436.99999630
CGAACTT303.592123E-430.83333213
TCGTGCA405.923271E-527.7513
ATTAGCT358.85144E-426.4285723
GTATTAG358.85144E-426.4285721
TAAAGAC1050.026.4285737
GGTATCA2950.025.7118641
GCGGTAA3150.025.2539723
TACCGCT451.3194868E-424.66666835
GGTAATA3350.024.29850825
AACTCCG3300.023.5454565
GTGCCAG3400.023.39705811
AGCCGCG3500.023.25714119
CGTGCCA3600.023.12500210
ACCGACC400.001927315423.1258
TAGGGGA400.001927315423.1254
ATACGGC400.001927315423.12529
TAATACG3150.022.90476227
GTAATAC3500.022.7285726
CGGTAAT3500.022.7285724
Produced by FastQC (version 0.11.5)