##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631934.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266378
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61496820307983	34.0	31.0	34.0	31.0	34.0
2	32.77304807454069	34.0	31.0	34.0	31.0	34.0
3	32.86284528001562	34.0	31.0	34.0	31.0	34.0
4	36.28773021796094	37.0	37.0	37.0	35.0	37.0
5	36.196851842119095	37.0	35.0	37.0	35.0	37.0
6	36.25249832944162	37.0	37.0	37.0	35.0	37.0
7	36.24840639992792	37.0	37.0	37.0	35.0	37.0
8	36.21922606221234	37.0	37.0	37.0	35.0	37.0
9	38.07548296030453	39.0	38.0	39.0	37.0	39.0
10	37.99400476015287	39.0	38.0	39.0	35.0	39.0
11	38.07860258730075	39.0	38.0	39.0	37.0	39.0
12	38.003641441860815	39.0	38.0	39.0	35.0	39.0
13	38.06217855828935	39.0	38.0	39.0	35.0	39.0
14	39.4411250178318	40.0	39.0	41.0	37.0	41.0
15	39.45441815765566	40.0	39.0	41.0	37.0	41.0
16	39.39476233022246	40.0	39.0	41.0	37.0	41.0
17	39.366700703511555	40.0	39.0	41.0	36.0	41.0
18	39.39043764875478	40.0	39.0	41.0	37.0	41.0
19	39.40130941744438	40.0	39.0	41.0	37.0	41.0
20	39.41464385196976	40.0	39.0	41.0	37.0	41.0
21	39.37541388553109	40.0	39.0	41.0	37.0	41.0
22	39.34560286510147	40.0	39.0	41.0	36.0	41.0
23	39.28998265622536	40.0	39.0	41.0	36.0	41.0
24	39.31020955183987	40.0	39.0	41.0	36.0	41.0
25	39.2497691250779	40.0	39.0	41.0	36.0	41.0
26	39.191742561322634	40.0	39.0	41.0	36.0	41.0
27	39.05368686603248	40.0	39.0	41.0	35.0	41.0
28	39.04985396691919	40.0	39.0	41.0	35.0	41.0
29	39.026023170081615	40.0	39.0	41.0	35.0	41.0
30	39.00940392975396	40.0	39.0	41.0	35.0	41.0
31	38.92053022396745	40.0	38.0	41.0	35.0	41.0
32	38.87914167085871	40.0	38.0	41.0	35.0	41.0
33	38.816756639061786	40.0	38.0	41.0	35.0	41.0
34	38.80695853261155	40.0	38.0	41.0	35.0	41.0
35	38.739246484319274	40.0	38.0	41.0	35.0	41.0
36	38.692722371967655	40.0	38.0	41.0	35.0	41.0
37	38.65880815983302	40.0	38.0	41.0	35.0	41.0
38	38.61164961070359	40.0	38.0	41.0	35.0	41.0
39	38.52497954035243	40.0	38.0	41.0	35.0	41.0
40	38.24299303996576	40.0	38.0	41.0	34.0	41.0
41	38.313659536448206	40.0	38.0	41.0	34.0	41.0
42	38.31128321407924	40.0	37.0	41.0	34.0	41.0
43	37.83580851271501	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	0.0
19	5.0
20	12.0
21	17.0
22	40.0
23	69.0
24	103.0
25	209.0
26	346.0
27	562.0
28	812.0
29	1181.0
30	1734.0
31	2356.0
32	3303.0
33	4536.0
34	6785.0
35	9903.0
36	15455.0
37	27622.0
38	64524.0
39	126802.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.5436785320109	16.20291465511416	12.96465924363123	28.288747569243704
2	20.760723483170533	19.7816636509021	32.05632597286563	27.401286893061737
3	20.824542567329136	19.663410642019986	29.580896320266685	29.931150470384193
4	15.393163099054727	15.922110684816312	34.71908340778893	33.96564280834003
5	16.20141302960455	33.40403486774433	34.01256860551547	16.38198349713565
6	33.549692542176906	34.204776670746085	15.81249202261448	16.43303876446253
7	29.919137466307276	27.939619638258417	20.119529390565287	22.02171350486902
8	27.358865972415142	30.634662021638427	20.929280946624722	21.077191059321716
9	28.550030407916573	12.485640706064315	17.154945228209538	41.809383657809576
10	18.97228750122007	23.54361095886297	29.617686145252236	27.866415394664724
11	39.441695635525456	19.202787016945845	18.680221339600116	22.675296007928583
12	22.590829573012787	23.936661436004474	26.576894488283564	26.89561450269917
13	35.46013559678352	17.548371111728443	20.731066379355653	26.260426912132385
14	23.93816306151409	19.892783938613547	23.28270352656751	32.886349473304854
15	29.624068053668097	24.145762788218246	19.901418285293833	26.32875087281983
16	25.43791153924123	24.36875417639595	22.31002560271494	27.883308681647883
17	27.329208868600258	23.867586662562225	21.41730923724932	27.385895231588194
18	26.53972925692062	21.367380189054654	23.25604967377186	28.836840880252872
19	28.15622911802026	22.332174578981746	23.33150635562997	26.180089947368025
20	30.537056363513503	20.4919325169496	22.53414321002485	26.436867909512046
21	27.381014948681948	22.0194610666046	21.596753485648215	29.00277049906524
22	28.597331611469418	22.430906456238876	21.07005833815105	27.90170359414066
23	28.009820630832877	22.196277470361668	22.034101915323337	27.759799983482118
24	27.97190458671512	21.385024288792618	22.849484567043827	27.793586557448435
25	28.149471803226994	21.847149539376375	22.962106480264886	27.04127217713175
26	28.236566082784613	22.59646066867384	22.27586362237122	26.89110962617033
27	26.746578170869967	21.524675461186735	24.417932411835814	27.310813956107488
28	26.127157648154125	22.960604854755275	22.00482021788586	28.90741727920474
29	27.177920098506636	23.671624533557576	22.265352243803918	26.88510312413187
30	26.694772090788277	22.02809541328488	23.647598525403748	27.62953397052309
31	28.265847780222092	22.58594929010654	22.774027885185713	26.374175044485654
32	25.648514516964617	22.265352243803918	22.921937997882708	29.16419524134876
33	26.06146153210851	21.672210167506325	24.712626418097592	27.55370188228758
34	26.590784524247496	21.849401977640795	23.735819024093583	27.823994474018125
35	25.58957571571226	23.89461592173528	24.39202937179497	26.123778990757497
36	26.228892776430484	22.319410762150028	24.910465578989253	26.541230882430234
37	27.0149937307135	22.137714075486713	24.260261733326328	26.587030460473464
38	26.115144644077215	21.686851016225063	25.436409913731616	26.761594425966106
39	25.509614157325306	20.521589620764477	26.207494612918484	27.761301608991733
40	25.18638926638086	21.478125070388696	27.256004625006568	26.07948103822388
41	23.227894195466593	20.81891147166808	27.979788120640592	27.973406212224734
42	21.94325357199168	21.082071342227962	29.20849319388238	27.76618189189798
43	21.063301023357788	21.96127307810705	28.911171342978776	28.06425455555639
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	5.0
4	8.0
5	8.0
6	6.0
7	4.0
8	6.5
9	9.0
10	31.0
11	53.0
12	53.0
13	93.0
14	133.0
15	248.0
16	363.0
17	383.0
18	403.0
19	403.0
20	446.0
21	489.0
22	429.5
23	370.0
24	428.5
25	487.0
26	487.0
27	592.5
28	698.0
29	972.5
30	1247.0
31	1457.5
32	1668.0
33	1668.0
34	2190.5
35	2713.0
36	3147.5
37	3582.0
38	4659.5
39	5737.0
40	5737.0
41	6951.5
42	8166.0
43	9400.0
44	10634.0
45	15506.5
46	20379.0
47	20379.0
48	22673.0
49	24967.0
50	28753.5
51	32540.0
52	33249.0
53	33958.0
54	33958.0
55	30014.5
56	26071.0
57	24564.5
58	23058.0
59	21550.0
60	20042.0
61	20042.0
62	18143.0
63	16244.0
64	13337.0
65	10430.0
66	9045.0
67	7660.0
68	7660.0
69	6409.5
70	5159.0
71	4368.5
72	3578.0
73	2919.5
74	2261.0
75	2261.0
76	1822.5
77	1384.0
78	1104.5
79	825.0
80	637.5
81	450.0
82	450.0
83	375.0
84	300.0
85	236.0
86	172.0
87	125.5
88	79.0
89	79.0
90	59.5
91	40.0
92	25.5
93	11.0
94	7.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	266378.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.8540292297647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88956382178851	45.05573982176526
2	5.892108400333484	6.110591224316693
3	2.0200420806605153	3.1424196328777514
4	1.0442595859488797	2.165962683730207
5	0.6785961195032095	1.7593971507962163
6	0.5050240974881396	1.5712520587731311
7	0.3673220392252997	1.3332989431107825
8	0.2653164678541591	1.1006182303397993
9	0.24072204312394282	1.1234167074357848
>10	1.8035605091904998	18.389501894720294
>50	0.19517464388798428	6.979459898170501
>100	0.09252723858393329	9.210113273788796
>500	0.0057829524114958305	2.0582284801747703
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	865	0.32472651645406153	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	811	0.3044545720742704	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	810	0.3040791656968668	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	657	0.2466419899541253	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	630	0.2365060177642298	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	612	0.22974870297096608	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	581	0.21811110527145636	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	525	0.19708834813685813	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	492	0.18469993768254137	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	484	0.18169668666331304	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	475	0.1783180292666812	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	465	0.1745639654926458	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	400	0.15016255096141573	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	393	0.14753470631959095	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	345	0.12951520020422108	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	345	0.12951520020422108	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	332	0.12463491729797506	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	332	0.12463491729797506	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	316	0.11862841525951842	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	314	0.11787760250471135	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	310	0.1163759769950972	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	296	0.11112028771144765	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	292	0.10961866220183349	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	290	0.1088678494470264	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	281	0.10548919205039455	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	279	0.10473837929558748	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	272	0.1021105346537627	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.754063774035393E-4	0.0
16	0.0	0.0	0.0	0.001126219132210618	0.0
17	0.0	0.0	0.0	0.001126219132210618	0.0
18	3.754063774035393E-4	0.0	0.0	0.001126219132210618	0.0
19	7.508127548070786E-4	0.0	0.0	0.0015016255096141572	0.0
20	7.508127548070786E-4	0.0	0.0	0.0026278446418247755	0.0
21	7.508127548070786E-4	0.0	0.0	0.0071327211706672475	0.0
22	7.508127548070786E-4	0.0	0.0	0.012763816831720337	0.0
23	7.508127548070786E-4	0.0	0.0	0.014640848718738034	0.0
24	7.508127548070786E-4	0.0	0.0	0.01614247422835219	0.0
25	7.508127548070786E-4	0.0	0.0	0.01726869336056281	0.0
26	7.508127548070786E-4	0.0	0.0	0.019896538002387585	0.0
27	7.508127548070786E-4	0.0	0.0	0.03678982498554685	0.0
28	7.508127548070786E-4	0.0	0.0	0.1088678494470264	0.0
29	7.508127548070786E-4	0.0	0.0	0.2233667945551059	0.0
30	7.508127548070786E-4	0.0	0.0	0.4084421386150508	0.0
31	0.001126219132210618	0.0	0.0	0.8837066124079316	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCT	25	1.2310808E-4	36.999996	30
CGAACTT	30	3.592123E-4	30.833332	13
TCGTGCA	40	5.923271E-5	27.75	13
ATTAGCT	35	8.85144E-4	26.428572	3
GTATTAG	35	8.85144E-4	26.428572	1
TAAAGAC	105	0.0	26.42857	37
GGTATCA	295	0.0	25.711864	1
GCGGTAA	315	0.0	25.25397	23
TACCGCT	45	1.3194868E-4	24.666668	35
GGTAATA	335	0.0	24.298508	25
AACTCCG	330	0.0	23.545456	5
GTGCCAG	340	0.0	23.397058	11
AGCCGCG	350	0.0	23.257141	19
CGTGCCA	360	0.0	23.125002	10
ACCGACC	40	0.0019273154	23.125	8
TAGGGGA	40	0.0019273154	23.125	4
ATACGGC	40	0.0019273154	23.125	29
TAATACG	315	0.0	22.904762	27
GTAATAC	350	0.0	22.72857	26
CGGTAAT	350	0.0	22.72857	24
>>END_MODULE