FastQCFastQC Report
Fri 10 Feb 2017
ERR1631933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275505
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4520.16406235821491444No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4490.16297344875773578No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4310.156439992014664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.1132465835465781No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2860.10380936825102993No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2810.10199451915573221No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2810.10199451915573221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.033.6865651
TGCACCA400.00192748223.1250025
GGATTAG502.6948823E-422.21
GTATCAA5100.021.7647062
GATTAGA450.003818538320.5555572
CGCGGTA2100.020.26190622
GCGGTAA2100.020.26190623
ATTCAAG656.877817E-519.9230751
CCGCGGT2150.019.79069721
AGCCGCG2250.019.73333419
CTTATAC2550.018.86274537
CGGCCTT701.2149333E-418.524
GTAGGCT500.00702164318.54
GTACTGG1800.018.51
AACTCCG2350.018.1063845
CGGTAAT2350.018.10638424
GCCGCGG2500.017.7620
TGAGCAA1054.7646245E-717.6190536
TCTTATA5050.017.5841637
TATACAC752.0600864E-417.26666837
Produced by FastQC (version 0.11.5)