##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631933.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	275505
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88185695359431	31.0	31.0	34.0	30.0	34.0
2	32.07581713580516	33.0	31.0	34.0	30.0	34.0
3	32.16494800457342	34.0	31.0	34.0	30.0	34.0
4	35.84660895446544	37.0	35.0	37.0	35.0	37.0
5	35.53010653164189	37.0	35.0	37.0	33.0	37.0
6	35.538545579935025	37.0	35.0	37.0	33.0	37.0
7	35.54662528810729	37.0	35.0	37.0	33.0	37.0
8	35.50701439175332	37.0	35.0	37.0	33.0	37.0
9	37.260263879058456	39.0	37.0	39.0	34.0	39.0
10	37.08788588228889	39.0	37.0	39.0	33.0	39.0
11	37.17796773198309	39.0	37.0	39.0	34.0	39.0
12	37.086223480517596	39.0	37.0	39.0	33.0	39.0
13	37.17833433150034	39.0	37.0	39.0	34.0	39.0
14	38.33132611023393	40.0	38.0	41.0	34.0	41.0
15	38.309213988856825	40.0	38.0	41.0	34.0	41.0
16	38.24072521369848	40.0	38.0	41.0	34.0	41.0
17	38.19585125496815	40.0	38.0	41.0	34.0	41.0
18	38.22346962850039	40.0	38.0	41.0	34.0	41.0
19	38.244227872452406	40.0	38.0	41.0	34.0	41.0
20	38.228609281138276	40.0	38.0	41.0	34.0	41.0
21	38.17729623781783	40.0	38.0	41.0	34.0	41.0
22	38.13267635796083	40.0	38.0	41.0	34.0	41.0
23	38.05420591277835	40.0	38.0	41.0	34.0	41.0
24	38.05788279704542	40.0	37.0	41.0	33.0	41.0
25	37.99200740458431	40.0	37.0	41.0	33.0	41.0
26	37.89481134643654	40.0	37.0	41.0	33.0	41.0
27	37.75274495925664	39.0	37.0	41.0	33.0	41.0
28	37.732295239650824	39.0	37.0	40.0	33.0	41.0
29	37.70271319939747	39.0	37.0	40.0	33.0	41.0
30	37.63746211502514	39.0	37.0	40.0	33.0	41.0
31	37.55314059635941	39.0	37.0	40.0	33.0	41.0
32	37.47833251665124	39.0	37.0	40.0	32.0	41.0
33	37.417114026968655	39.0	36.0	40.0	32.0	41.0
34	37.3714016079563	39.0	36.0	40.0	32.0	41.0
35	37.27784250739551	39.0	36.0	40.0	32.0	41.0
36	37.220700168780965	39.0	36.0	40.0	32.0	41.0
37	37.1597067203862	39.0	36.0	40.0	32.0	41.0
38	37.11816845429303	39.0	36.0	40.0	31.0	41.0
39	37.0441153518085	39.0	36.0	40.0	31.0	41.0
40	36.744483766174845	39.0	35.0	40.0	31.0	41.0
41	36.8103845665233	39.0	35.0	40.0	31.0	41.0
42	36.77895500989093	39.0	35.0	40.0	31.0	41.0
43	36.21871472387071	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	2.0
17	3.0
18	12.0
19	17.0
20	34.0
21	59.0
22	109.0
23	231.0
24	360.0
25	546.0
26	885.0
27	1356.0
28	1881.0
29	2641.0
30	3665.0
31	5018.0
32	6701.0
33	9303.0
34	13193.0
35	19808.0
36	31149.0
37	52868.0
38	80605.0
39	45058.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.73388867715649	17.348142501950964	13.061105969038675	26.85686285185387
2	20.809422696502786	20.364421698335782	33.01355692274187	25.81259868241956
3	21.2301047167928	20.658064281954953	29.64918966987895	28.462641331373295
4	15.95288651748607	16.32311573292681	33.67053229523965	34.05346545434747
5	16.654870147547232	33.63278343405746	33.08832870546814	16.62401771292717
6	33.923522259124155	34.07814740204352	15.709333768897116	16.28899656993521
7	30.126494981942255	28.535235295185206	19.6976461407234	21.640623582149143
8	27.072466924375238	31.700332117384438	20.082031179107457	21.145169779132868
9	27.71274568519628	13.617901671476018	17.84613709370066	40.82321554962705
10	19.03195949256819	24.34220794540934	30.426671022304497	26.199161539717974
11	38.310375492277814	20.27259033411372	19.76769931580189	21.649334857806572
12	22.572367107675	24.01589807807481	27.43761456234914	25.974120251901056
13	34.20119417070471	17.9256274840747	21.973103936407686	25.900074408812905
14	23.78287145423858	19.96733271628464	23.74911526106604	32.500680568410736
15	28.48187873178345	25.121141177111124	20.546995517322735	25.84998457378269
16	25.33747118927061	24.73094862162211	22.67581350610697	27.25576668300031
17	26.172664742926628	24.24602094335856	22.776356145986462	26.804958167728355
18	25.859784758897298	22.461661312861835	24.10083301573474	27.577720912506127
19	27.92544599916517	22.924084862343697	23.784686303333878	25.36578283515726
20	28.69966062321918	22.04315711148618	23.301573474165625	25.95560879112902
21	27.415836373205565	22.997767735612783	22.3309921780004	27.25540371318125
22	27.32690876753598	23.60792000145188	21.979637393150757	27.08553383786138
23	27.070289105460883	23.132429538483876	22.990871309050654	26.80641004700459
24	27.251774014990655	22.62463476161957	23.186149071704687	26.937442151685087
25	27.551587085533836	22.704488121812673	23.522259124153827	26.221665668499668
26	27.25540371318125	23.08887316019673	23.197764105914594	26.457959020707428
27	26.933086513856374	22.525181031197256	23.906281192718826	26.635451262227544
28	26.241991978366997	23.71681094716974	22.879802544418432	27.161394530044824
29	26.39879494020072	24.00646086277926	22.868550480027587	26.72619371699243
30	26.377742690695268	23.713181248979147	23.953104299377507	25.955971760948078
31	27.251411045171594	22.98397488248852	23.26309867334531	26.501515398994574
32	24.85181757136894	23.27180994900274	23.780330665505165	28.096041814123158
33	25.491733362370915	22.83443131703599	24.633309740295097	27.040525580298
34	25.61804685940364	22.548411099617066	24.65291011052431	27.180631930454986
35	25.733834231683634	23.52044427505853	24.714977949583492	26.030743543674344
36	25.661240267871726	22.805393731511224	25.384657265748352	26.1487087348687
37	26.17121286365039	22.022467831799787	25.036569209270247	26.769750095279576
38	24.90626304422787	21.790530117420737	26.355601531732635	26.947605306618755
39	24.544745104444566	20.878749931943158	27.19297290430301	27.383532059309267
40	24.16290085479392	21.565851799422877	28.088056478103844	26.18319086767935
41	23.12226638355021	21.00361154969964	28.852107947224187	27.022014119525963
42	22.15277399684216	21.200704161448975	29.300738643581788	27.345783198127076
43	21.272572185622764	21.702691421208325	29.26480463149489	27.759931761674018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	5.5
4	8.0
5	8.0
6	17.0
7	26.0
8	34.0
9	42.0
10	58.5
11	75.0
12	75.0
13	148.0
14	221.0
15	424.0
16	627.0
17	627.0
18	627.0
19	627.0
20	724.5
21	822.0
22	799.0
23	776.0
24	958.5
25	1141.0
26	1141.0
27	1291.5
28	1442.0
29	2039.0
30	2636.0
31	3062.0
32	3488.0
33	3488.0
34	4108.0
35	4728.0
36	5367.0
37	6006.0
38	7181.5
39	8357.0
40	8357.0
41	9819.0
42	11281.0
43	12448.5
44	13616.0
45	17037.5
46	20459.0
47	20459.0
48	22526.0
49	24593.0
50	26615.5
51	28638.0
52	29416.5
53	30195.0
54	30195.0
55	27660.5
56	25126.0
57	23538.0
58	21950.0
59	20228.5
60	18507.0
61	18507.0
62	16828.0
63	15149.0
64	12834.0
65	10519.0
66	9206.5
67	7894.0
68	7894.0
69	6875.5
70	5857.0
71	5029.0
72	4201.0
73	3440.5
74	2680.0
75	2680.0
76	2162.0
77	1644.0
78	1347.5
79	1051.0
80	788.5
81	526.0
82	526.0
83	421.5
84	317.0
85	237.5
86	158.0
87	121.0
88	84.0
89	84.0
90	56.0
91	28.0
92	14.0
93	0.0
94	2.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	275505.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.53669426934406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48103333487309	55.95848024567942
2	5.0986481904577134	6.377052061471968
3	1.6180005196188192	3.0355321146912573
4	0.8695572254109373	2.1751693742089153
5	0.5301455861617872	1.6576776220020943
6	0.38702459530261196	1.4521943274693634
7	0.25481134199683236	1.1154541293572025
8	0.21999537349080076	1.1006226730131492
9	0.1484474204523903	0.8355069853113514
>10	1.2219988957734675	14.885967646646664
>50	0.11124082707692359	4.914934098513902
>100	0.05909668938461565	6.491408721634725
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	452	0.16406235821491444	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	449	0.16297344875773578	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	431	0.156439992014664	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	312	0.1132465835465781	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	286	0.10380936825102993	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	281	0.10199451915573221	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	281	0.10199451915573221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.629698190595452E-4
7	0.0	0.0	0.0	0.0	3.629698190595452E-4
8	0.0	0.0	0.0	0.0	3.629698190595452E-4
9	0.0	0.0	0.0	0.0	3.629698190595452E-4
10	0.0	0.0	0.0	0.0	3.629698190595452E-4
11	0.0	0.0	0.0	0.0	3.629698190595452E-4
12	0.0	0.0	0.0	0.0	3.629698190595452E-4
13	0.0	0.0	0.0	0.0	3.629698190595452E-4
14	0.0	0.0	0.0	3.629698190595452E-4	3.629698190595452E-4
15	0.0	0.0	0.0	3.629698190595452E-4	3.629698190595452E-4
16	0.0	0.0	0.0	7.259396381190904E-4	3.629698190595452E-4
17	3.629698190595452E-4	0.0	0.0	0.0010889094571786355	3.629698190595452E-4
18	3.629698190595452E-4	0.0	0.0	0.0010889094571786355	3.629698190595452E-4
19	3.629698190595452E-4	0.0	0.0	0.0010889094571786355	3.629698190595452E-4
20	3.629698190595452E-4	0.0	0.0	0.001814849095297726	3.629698190595452E-4
21	3.629698190595452E-4	0.0	0.0	0.0029037585524763617	3.629698190595452E-4
22	3.629698190595452E-4	0.0	0.0	0.0032667283715359066	3.629698190595452E-4
23	3.629698190595452E-4	0.0	0.0	0.003992668009654997	7.259396381190904E-4
24	3.629698190595452E-4	0.0	0.0	0.005081577466833633	7.259396381190904E-4
25	3.629698190595452E-4	0.0	0.0	0.005444547285893178	7.259396381190904E-4
26	3.629698190595452E-4	0.0	0.0	0.008348305838369539	7.259396381190904E-4
27	3.629698190595452E-4	0.0	0.0	0.02577085715322771	7.259396381190904E-4
28	3.629698190595452E-4	0.0	0.0	0.12159488938494764	7.259396381190904E-4
29	3.629698190595452E-4	0.0	0.0	0.28239051922832614	7.259396381190904E-4
30	3.629698190595452E-4	0.0	0.0	0.47186076477740874	7.259396381190904E-4
31	3.629698190595452E-4	0.0	0.0	1.0108709460808334	7.259396381190904E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	335	0.0	33.686565	1
TGCACCA	40	0.001927482	23.125002	5
GGATTAG	50	2.6948823E-4	22.2	1
GTATCAA	510	0.0	21.764706	2
GATTAGA	45	0.0038185383	20.555557	2
CGCGGTA	210	0.0	20.261906	22
GCGGTAA	210	0.0	20.261906	23
ATTCAAG	65	6.877817E-5	19.923075	1
CCGCGGT	215	0.0	19.790697	21
AGCCGCG	225	0.0	19.733334	19
CTTATAC	255	0.0	18.862745	37
CGGCCTT	70	1.2149333E-4	18.5	24
GTAGGCT	50	0.007021643	18.5	4
GTACTGG	180	0.0	18.5	1
AACTCCG	235	0.0	18.106384	5
CGGTAAT	235	0.0	18.106384	24
GCCGCGG	250	0.0	17.76	20
TGAGCAA	105	4.7646245E-7	17.61905	36
TCTTATA	505	0.0	17.58416	37
TATACAC	75	2.0600864E-4	17.266668	37
>>END_MODULE