FastQCFastQC Report
Fri 10 Feb 2017
ERR1631932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546187
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9460.17320075358805684No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8750.16020154269508427No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8670.1587368428761578No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8610.15763831801196293No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7680.14061118261694255No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7400.13548473325069985No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7190.13163989622601782No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6720.12303478478982474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.11333114848943676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6130.1122326236252419No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6120.1120495361478761No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6070.11113409876104703No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6000.10985248641948638No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5780.10582456191743854No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5580.10216281237012233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA308.303996E-637.013
GGTATCA7600.031.6447371
TAAGATA250.00549384929.64
GCTTAAC509.079686E-625.90000215
CGAAAAA451.3219705E-424.66666623
TAAACGG451.3219705E-424.6666665
GCCCAAT400.001929896223.1250021
CTAGCAC400.001929896223.1250023
CGCTTAA603.7216792E-521.58333214
AAAGCGA1151.3460522E-1020.91304420
TATACAC1700.020.67647237
CATATCT450.003823276420.5555553
CGAACTA1101.7480488E-920.18181824
TACGCTT555.138344E-420.1818184
AGTCGCT555.138344E-420.18181811
GGACCGT759.251178E-619.7333346
GTATCAA12300.019.5528452
TAATACT951.6720878E-719.4736844
CTTATAC5900.019.44067637
GCGAACT1153.0395313E-919.30434823
Produced by FastQC (version 0.11.5)