FastQCFastQC Report
Fri 10 Feb 2017
ERR1631931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147633
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA33650.293212202855791No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA32060.27935759951134204No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA32030.27909619190106943No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA31740.2765692516684341No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC28000.2439804362544472No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA26290.22908020246890773No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG25170.21932098501872985No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC23680.20633774037518965No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT23380.20372366427246338No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA21520.18751639243556084No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA20900.18211396848992664No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT20210.17610159345365634No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17770.1548404411514831No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15860.13819748996412617No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15270.13305647362876458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15150.13201084318767412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15140.13192370731758324No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA14930.1300938540456749No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA14810.1290482236045844No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA14310.12469143010004069No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14080.1226873050879506No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13580.11833051158340688No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT13390.11667493005168028No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA13170.11475794090968106No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC12770.1112725061060461No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT12490.10883270174350161No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG12360.10769993543232026No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12130.10569581042023016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12100.10543440280995754No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11980.10438877236886705No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA11960.1042145006286853No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG11720.10212323974650432No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT11630.10133901691568646No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT11590.10099047343532297No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA11540.10055479408486859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17750.028.9746481
ATAATAC358.869389E-426.428573
TAGACAG509.091073E-625.9000025
ATACGGC2300.024.13043629
TTTTACG1155.456968E-1222.521744
TACGGCT2550.021.76470630
CTTATAC11000.020.3537
GCGGTAA13950.019.89247323
CGCGGTA14550.019.3264622
TTAGCTA1153.0504452E-919.3043484
GTAACGT8450.018.82840226
AACTCCG15000.018.6233335
AGCCGCG14950.018.56187219
GGTAATA15100.018.525
GTATTAG2500.018.51
TAGTTCC1601.8189894E-1218.54
TTATACC500.00703487618.54
GTATCAA27600.018.52
TAGGACA902.15221E-618.54
TTCGCTA902.15221E-618.526
Produced by FastQC (version 0.11.5)