##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631931.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1147633
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5920028441148	34.0	31.0	34.0	31.0	34.0
2	32.75743987842803	34.0	31.0	34.0	31.0	34.0
3	32.85080857730651	34.0	31.0	34.0	31.0	34.0
4	36.26759077161427	37.0	37.0	37.0	35.0	37.0
5	36.190485111529554	37.0	35.0	37.0	35.0	37.0
6	36.24626165333343	37.0	37.0	37.0	35.0	37.0
7	36.246462065834635	37.0	37.0	37.0	35.0	37.0
8	36.22392350167693	37.0	37.0	37.0	35.0	37.0
9	38.06380262679794	39.0	38.0	39.0	35.0	39.0
10	37.98891631732444	39.0	38.0	39.0	35.0	39.0
11	38.071379090702344	39.0	38.0	39.0	37.0	39.0
12	37.99574689818086	39.0	38.0	39.0	35.0	39.0
13	38.05663918691777	39.0	38.0	39.0	35.0	39.0
14	39.43118052548158	40.0	39.0	41.0	37.0	41.0
15	39.44584984921138	40.0	39.0	41.0	37.0	41.0
16	39.388712245116686	40.0	39.0	41.0	37.0	41.0
17	39.35594131573421	40.0	39.0	41.0	36.0	41.0
18	39.38523988069357	40.0	39.0	41.0	37.0	41.0
19	39.4017608416628	40.0	39.0	41.0	37.0	41.0
20	39.40220262052416	40.0	39.0	41.0	37.0	41.0
21	39.36222555468517	40.0	39.0	41.0	36.0	41.0
22	39.33121738395462	40.0	39.0	41.0	36.0	41.0
23	39.27290867376592	40.0	39.0	41.0	36.0	41.0
24	39.29054845930711	40.0	39.0	41.0	36.0	41.0
25	39.232874969611366	40.0	39.0	41.0	36.0	41.0
26	39.176222712313084	40.0	39.0	41.0	36.0	41.0
27	39.04883268431633	40.0	39.0	41.0	35.0	41.0
28	39.05141190607102	40.0	39.0	41.0	35.0	41.0
29	39.024140121449975	40.0	39.0	41.0	35.0	41.0
30	38.99099625054351	40.0	38.0	41.0	35.0	41.0
31	38.90016320548468	40.0	38.0	41.0	35.0	41.0
32	38.85493533211401	40.0	38.0	41.0	35.0	41.0
33	38.79143593814399	40.0	38.0	41.0	35.0	41.0
34	38.77570094272298	40.0	38.0	41.0	35.0	41.0
35	38.705217608765174	40.0	38.0	41.0	35.0	41.0
36	38.654324161121195	40.0	38.0	41.0	35.0	41.0
37	38.61180098515815	40.0	38.0	41.0	35.0	41.0
38	38.57139782491441	40.0	38.0	41.0	35.0	41.0
39	38.48281724209743	40.0	38.0	41.0	34.0	41.0
40	38.192744544641016	40.0	38.0	41.0	34.0	41.0
41	38.25025073346619	40.0	37.0	41.0	34.0	41.0
42	38.23287845504617	40.0	37.0	41.0	34.0	41.0
43	37.75809252609501	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	0.0
15	2.0
16	0.0
17	3.0
18	5.0
19	22.0
20	33.0
21	74.0
22	163.0
23	345.0
24	572.0
25	916.0
26	1457.0
27	2422.0
28	3550.0
29	5484.0
30	7583.0
31	10568.0
32	14569.0
33	19682.0
34	29081.0
35	43512.0
36	66965.0
37	121419.0
38	278055.0
39	541148.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.87651191626591	16.264084424201812	12.758085555225406	28.101318104306866
2	20.669412608386132	19.636504004328913	32.57557076173306	27.1185126255519
3	21.019001719190715	19.682511743736892	29.691286325855042	29.60720021121735
4	15.051065976666756	16.275586359053808	35.02321735258571	33.65013031169372
5	16.402891865256574	33.08531560176468	34.02446600960411	16.48732652337463
6	32.82887473608723	34.61106468705588	16.024896460802367	16.535164116054524
7	29.876711457408422	28.033264989765893	20.283052160403194	21.80697139242249
8	27.239457213238026	30.419306520464296	20.79035719607226	21.550879070225413
9	28.70516968403662	12.600892445581472	17.740078927671128	40.95385894271078
10	19.05757328344514	23.275123667583628	29.70810354878258	27.959199500188646
11	38.96315285461468	19.586226607286473	18.97252867423645	22.4780918638624
12	22.364553825133992	24.086271482259573	27.21636620766395	26.33280848494249
13	35.34440016974067	17.7460913027074	20.865032636740143	26.044475890811785
14	23.836975757929583	20.36739968265116	23.65433897421911	32.141285585200144
15	29.616523749317075	24.162253960978813	20.26980750814938	25.951414781554732
16	24.687334714146424	24.531012963203395	22.98748816041365	27.794164162236534
17	26.643970677037	24.05481543315677	21.92957156164035	27.371642328165883
18	26.547336997106218	21.324848623209686	23.635343354539298	28.4924710251448
19	27.879034499705046	22.549281869726645	23.768835507518517	25.80284812304979
20	30.200246943055838	20.773539973144725	22.87638992604779	26.14982315775165
21	27.257842881827205	22.172767775063978	22.244132052668405	28.32525729044041
22	28.3091371544736	22.942264643836488	21.597061081373575	27.151537120316338
23	27.885046874741313	22.06672342116339	22.662994180195238	27.385235523900064
24	27.47794808967675	21.57153027143695	23.46795534809473	27.482566290791567
25	27.695090677943213	22.13590930201554	23.426217266321203	26.742782753720046
26	27.65631521575277	22.968841084214205	22.60051776134008	26.774325938692943
27	26.645277715088362	21.866572327564647	24.693869904403236	26.794280052943755
28	26.08386130409286	23.127079824299233	22.290749743167023	28.498309128440884
29	26.98502047257268	23.65198630572666	22.67467038678741	26.688322834913254
30	26.73502766128196	22.0770925897042	24.00828487852824	27.1795948704856
31	27.787280428499354	22.687740767301044	23.034454394392633	26.49052440980697
32	25.463453909045835	22.42005937438188	23.30222292318189	28.814263793390392
33	25.674584122276023	22.009562290383773	25.069512640364994	27.246340946975213
34	26.256128919262515	22.11795931277682	24.16687216209363	27.45903960586703
35	25.39723064777677	23.948945350996357	24.69247573048178	25.961348270745088
36	26.085342613884404	22.588144467787174	25.07665778171245	26.249855136615974
37	26.755940270103768	22.419710830901515	24.47864430527878	26.345704593715936
38	25.908543933470018	21.91676258873699	25.265829755679736	26.908863722113253
39	25.371699837840143	20.956089620985104	26.15966951107192	27.512541030102827
40	25.118308727615883	21.869099267797285	26.945025108200966	26.067566896385863
41	23.34622653757778	21.09794681749305	27.88025440188632	27.675572243042858
42	21.988649681561963	21.557239988742047	28.91690984835745	27.537200481338548
43	21.28964573169297	22.3692591621189	28.533076340607145	27.808018765580982
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	10.0
2	14.0
3	29.5
4	45.0
5	45.0
6	66.5
7	88.0
8	110.5
9	133.0
10	196.5
11	260.0
12	260.0
13	533.0
14	806.0
15	1682.0
16	2558.0
17	2515.5
18	2473.0
19	2473.0
20	2801.5
21	3130.0
22	2616.0
23	2102.0
24	2354.0
25	2606.0
26	2606.0
27	3107.0
28	3608.0
29	4859.0
30	6110.0
31	7419.5
32	8729.0
33	8729.0
34	10986.0
35	13243.0
36	15239.0
37	17235.0
38	21583.0
39	25931.0
40	25931.0
41	31485.0
42	37039.0
43	43440.0
44	49841.0
45	69902.0
46	89963.0
47	89963.0
48	99869.5
49	109776.0
50	123923.5
51	138071.0
52	139127.5
53	140184.0
54	140184.0
55	125858.0
56	111532.0
57	104587.5
58	97643.0
59	90534.0
60	83425.0
61	83425.0
62	75686.5
63	67948.0
64	55503.0
65	43058.0
66	36867.0
67	30676.0
68	30676.0
69	26094.0
70	21512.0
71	18253.0
72	14994.0
73	11960.0
74	8926.0
75	8926.0
76	7332.0
77	5738.0
78	4654.5
79	3571.0
80	2772.0
81	1973.0
82	1973.0
83	1622.0
84	1271.0
85	989.5
86	708.0
87	556.0
88	404.0
89	404.0
90	299.5
91	195.0
92	137.0
93	79.0
94	51.5
95	24.0
96	24.0
97	14.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1147633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.79220776130446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.87872669015914	38.914899759431954
2	6.463292226395623	5.790102564534736
3	2.009377478685872	2.7001336048855107
4	1.0166459903322695	1.8215127367464057
5	0.632474178756907	1.4164957409269898
6	0.426461235720821	1.146128416352979
7	0.3113831477024646	0.9763277051680422
8	0.22432864466234828	0.8038540206822197
9	0.17998220851820174	0.725562042955714
>10	1.4649981100807563	13.448617450919917
>50	0.20790318507368605	6.617477429452991
>100	0.15998007153432675	14.146442515484692
>500	0.014081375449506457	4.274900128531596
>1k	0.01036545692810892	7.217545883926273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	3365	0.293212202855791	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3206	0.27935759951134204	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	3203	0.27909619190106943	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3174	0.2765692516684341	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2800	0.2439804362544472	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2629	0.22908020246890773	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2517	0.21932098501872985	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	2368	0.20633774037518965	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2338	0.20372366427246338	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	2152	0.18751639243556084	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	2090	0.18211396848992664	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	2021	0.17610159345365634	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1777	0.1548404411514831	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1586	0.13819748996412617	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1527	0.13305647362876458	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1515	0.13201084318767412	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1514	0.13192370731758324	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1493	0.1300938540456749	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1481	0.1290482236045844	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1431	0.12469143010004069	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1408	0.1226873050879506	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1358	0.11833051158340688	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1339	0.11667493005168028	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1317	0.11475794090968106	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1277	0.1112725061060461	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	1249	0.10883270174350161	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	1236	0.10769993543232026	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1213	0.10569581042023016	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1210	0.10543440280995754	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	1198	0.10438877236886705	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1196	0.1042145006286853	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	1172	0.10212323974650432	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	1163	0.10133901691568646	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	1159	0.10099047343532297	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	1154	0.10055479408486859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.7135870090874E-5	0.0	0.0	0.0	0.0
3	8.7135870090874E-5	0.0	0.0	0.0	0.0
4	8.7135870090874E-5	0.0	0.0	0.0	0.0
5	8.7135870090874E-5	0.0	0.0	0.0	0.0
6	8.7135870090874E-5	0.0	0.0	0.0	0.0
7	8.7135870090874E-5	0.0	0.0	0.0	0.0
8	4.3567935045437E-4	0.0	0.0	0.0	0.0
9	4.3567935045437E-4	0.0	0.0	8.7135870090874E-5	0.0
10	4.3567935045437E-4	0.0	0.0	8.7135870090874E-5	0.0
11	4.3567935045437E-4	0.0	0.0	8.7135870090874E-5	0.0
12	4.3567935045437E-4	0.0	0.0	8.7135870090874E-5	0.0
13	6.09951090636118E-4	0.0	0.0	8.7135870090874E-5	0.0
14	6.09951090636118E-4	0.0	0.0	1.74271740181748E-4	0.0
15	6.97086960726992E-4	0.0	0.0	3.48543480363496E-4	0.0
16	6.97086960726992E-4	0.0	0.0	7.84222830817866E-4	0.0
17	6.97086960726992E-4	0.0	0.0	0.00130703805136311	0.0
18	8.7135870090874E-4	0.0	0.0	0.002004125012090102	0.0
19	8.7135870090874E-4	0.0	0.0	0.002788347842907968	0.0
20	9.58494570999614E-4	0.0	0.0	0.004269657634452826	0.0
21	0.001045630441090488	0.0	0.0	0.007145141347451668	0.0
22	0.001045630441090488	0.0	0.0	0.011153391371631871	0.0
23	0.001045630441090488	0.0	0.0	0.013767467474358091	0.0
24	0.001045630441090488	0.0	0.0	0.01681722292753868	0.0
25	0.001045630441090488	0.0	0.0	0.018211396848992666	0.0
26	0.001045630441090488	0.0	0.0	0.021609695782536752	0.0
27	0.001132766311181362	0.0	0.0	0.0372070165288032	0.0
28	0.001132766311181362	0.0	0.0	0.10587008216041191	0.0
29	0.001132766311181362	0.0	0.0	0.22263214808218307	0.0
30	0.001132766311181362	0.0	0.0	0.38200365447839163	0.0
31	0.001132766311181362	0.0	0.0	0.8536701192802926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1775	0.0	28.974648	1
ATAATAC	35	8.869389E-4	26.42857	3
TAGACAG	50	9.091073E-6	25.900002	5
ATACGGC	230	0.0	24.130436	29
TTTTACG	115	5.456968E-12	22.52174	4
TACGGCT	255	0.0	21.764706	30
CTTATAC	1100	0.0	20.35	37
GCGGTAA	1395	0.0	19.892473	23
CGCGGTA	1455	0.0	19.32646	22
TTAGCTA	115	3.0504452E-9	19.304348	4
GTAACGT	845	0.0	18.828402	26
AACTCCG	1500	0.0	18.623333	5
AGCCGCG	1495	0.0	18.561872	19
GGTAATA	1510	0.0	18.5	25
GTATTAG	250	0.0	18.5	1
TAGTTCC	160	1.8189894E-12	18.5	4
TTATACC	50	0.007034876	18.5	4
GTATCAA	2760	0.0	18.5	2
TAGGACA	90	2.15221E-6	18.5	4
TTCGCTA	90	2.15221E-6	18.5	26
>>END_MODULE