FastQCFastQC Report
Fri 10 Feb 2017
ERR1631930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409671
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15100.3685884526852035No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13620.3324619023557928No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13130.3205010850170015No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12780.3119576440607219No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10950.2672876527750317No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10530.2570355236274962No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10020.24458650966263173No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9270.22627913618488982No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9190.22432634968059736No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9110.22237356317630486No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8340.2035779930724899No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA7960.19430225717710065No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7360.17965635839490712No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6740.1645222629866405No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6550.1598843950389459No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6310.15402603552606847No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6240.1523173473348126No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6140.149876364204447No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6030.14719128276104484No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5970.14572669288282547No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5920.1445062013176427No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5860.14304161143942334No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5850.14279751312638678No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5660.13815964517869217No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5410.1320571873527782No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5320.12986030253544917No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC5290.1291280075963395No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC5250.12815161434419328No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG5200.12693112277901047No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5130.12522243458775456No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4960.12107276326613306No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4910.11985227170095028No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4820.11765538688362125No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4820.11765538688362125No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4790.11692309194451159No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4740.11570260037932878No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4690.11448210881414599No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4680.11423801050110943No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4660.1137498138750363No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4650.11350571556199975No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4630.11301751893592663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4620.11277342062289007No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4620.11277342062289007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.11252932230985352No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG4610.11252932230985352No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4550.11106473243163416No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4540.1108206341185976No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA4500.10984424086645138No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4480.10935604424037826No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4470.1091119459273417No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4420.1078914543621589No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA4420.1078914543621589No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4310.10520637291875676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC200.001840553837.03
GTTCTAG303.5954703E-430.8333341
TAGACAT250.00549226729.5999985
TGGTCGA250.00549226729.59999810
CACTTGC250.00549226729.5999981
GGTATCA6750.028.7777771
CTAGACA405.9309434E-527.754
ATTAGAG358.8596134E-426.428573
ATACGGC1350.026.03703729
TATTAGA400.001929077823.1252
TACGGCT1550.022.67741830
TGGCAAC502.6979914E-422.19999925
CTTATAC3700.021.537
TCTTCTG4000.021.27499816
TTTAAGC705.0887065E-621.1428572
TAATGTT705.0887065E-621.14285731
GCAGTCG1500.020.9666679
TAAGTCT555.13533E-420.18181835
GTCTTGG555.13533E-420.1818181
CTCTATG2200.020.1818181
Produced by FastQC (version 0.11.5)