##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631930.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409671
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58304590756973	34.0	31.0	34.0	31.0	34.0
2	32.750436325734555	34.0	31.0	34.0	31.0	34.0
3	32.84188043576431	34.0	31.0	34.0	31.0	34.0
4	36.258573342999625	37.0	37.0	37.0	35.0	37.0
5	36.18480927378311	37.0	35.0	37.0	35.0	37.0
6	36.238908782901404	37.0	37.0	37.0	35.0	37.0
7	36.23862562885828	37.0	37.0	37.0	35.0	37.0
8	36.2198007669569	37.0	37.0	37.0	35.0	37.0
9	38.06167632075495	39.0	38.0	39.0	35.0	39.0
10	37.98892037757127	39.0	38.0	39.0	35.0	39.0
11	38.069419119244465	39.0	38.0	39.0	37.0	39.0
12	37.985524970036934	39.0	38.0	39.0	35.0	39.0
13	38.05562512357477	39.0	38.0	39.0	35.0	39.0
14	39.41731535793356	40.0	39.0	41.0	37.0	41.0
15	39.43920853562981	40.0	39.0	41.0	37.0	41.0
16	39.37128329806113	40.0	39.0	41.0	36.0	41.0
17	39.34922413351201	40.0	39.0	41.0	36.0	41.0
18	39.388489788147076	40.0	39.0	41.0	37.0	41.0
19	39.393911211679615	40.0	39.0	41.0	37.0	41.0
20	39.40002099245492	40.0	39.0	41.0	37.0	41.0
21	39.355448640494444	40.0	39.0	41.0	36.0	41.0
22	39.326745119864476	40.0	39.0	41.0	36.0	41.0
23	39.26886208689412	40.0	39.0	41.0	36.0	41.0
24	39.28222402854974	40.0	39.0	41.0	36.0	41.0
25	39.22851751771544	40.0	39.0	41.0	36.0	41.0
26	39.176614893414474	40.0	39.0	41.0	36.0	41.0
27	39.027312160245664	40.0	39.0	41.0	35.0	41.0
28	39.0402493708366	40.0	39.0	41.0	35.0	41.0
29	39.020257718998906	40.0	39.0	41.0	35.0	41.0
30	38.992342635920046	40.0	38.0	41.0	35.0	41.0
31	38.90828982280903	40.0	38.0	41.0	35.0	41.0
32	38.87407944423696	40.0	38.0	41.0	35.0	41.0
33	38.80692555733747	40.0	38.0	41.0	35.0	41.0
34	38.781842014689836	40.0	38.0	41.0	35.0	41.0
35	38.71161737101235	40.0	38.0	41.0	35.0	41.0
36	38.67155839685992	40.0	38.0	41.0	35.0	41.0
37	38.624337578203	40.0	38.0	41.0	35.0	41.0
38	38.58313378296243	40.0	38.0	41.0	35.0	41.0
39	38.49231700559717	40.0	38.0	41.0	34.0	41.0
40	38.19323066558287	40.0	37.0	41.0	34.0	41.0
41	38.25761403662939	40.0	37.0	41.0	34.0	41.0
42	38.246690637121006	40.0	37.0	41.0	34.0	41.0
43	37.772266037869414	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	11.0
21	23.0
22	52.0
23	95.0
24	179.0
25	317.0
26	502.0
27	801.0
28	1306.0
29	1890.0
30	2655.0
31	3829.0
32	5287.0
33	7157.0
34	10451.0
35	15572.0
36	24225.0
37	42920.0
38	99962.0
39	192431.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.88953819040157	16.21032487044482	12.6894508032055	28.210686135948116
2	21.31246780953497	19.44682440299655	31.88436574714832	27.356342040320158
3	21.264380441866766	19.372862614146474	29.46144589194744	29.90131105203932
4	15.14361524247506	16.39266631028313	35.23705607670545	33.22666237053636
5	16.685340187613964	32.714788208098696	33.75147374356496	16.848397860722383
6	32.493146939861504	34.554068996829166	16.134898491716523	16.817885571592814
7	29.65599224743758	27.633393625616655	20.097590505552017	22.61302362139375
8	27.093448157179782	29.950618911272702	20.884807565094917	22.07112536645259
9	28.69107161600406	12.329893988102649	17.539196086615846	41.43983830927744
10	19.623307483321984	22.78413653883238	28.96348533335286	28.629070644492778
11	39.64644800339785	19.095322832223907	18.730151755921217	22.52807740845703
12	22.236135826065308	23.98265925584188	26.91965015829776	26.86155475979505
13	36.20539408452148	17.500384454843033	20.085385589900188	26.208835870735296
14	23.747104383761602	20.45690322234183	23.443446082344124	32.35254631155244
15	30.283569010254567	23.576235564636015	19.901091363557587	26.23910406155183
16	24.612921100102277	24.262395922581778	23.055573862929034	28.069109114386908
17	27.031203087355465	23.66581964552043	21.407910249932264	27.895067017191842
18	26.705820036077732	20.79473528758443	23.176646626195165	29.322798050142673
19	28.120613858437626	22.234915334500123	23.44246968909198	26.202001117970276
20	30.74662351008492	20.122732631794783	22.424091527103457	26.70655233101684
21	27.748608029369908	21.828979839920326	21.676418394272474	28.745993736437285
22	28.803600938313917	22.658914104244626	21.149410136426546	27.388074821014914
23	28.574392622372585	21.5243451452507	22.220025337404895	27.681236894971818
24	27.97903683687642	20.997825084030843	23.016518132843185	28.00661994624955
25	28.043966988144142	21.735490186027324	23.20618252207259	27.01436030375594
26	28.03591174381394	22.662575578940174	22.330357774897415	26.97115490234847
27	26.927949500941	21.47699007252161	24.65319732175331	26.94186310478408
28	26.277427496698568	23.008951085139053	21.80896377825133	28.90465763991105
29	27.521840696558947	23.50349426735112	22.114330767860064	26.860334268229874
30	27.109070449214123	21.381303533811277	23.905280090609292	27.604345926365305
31	28.267316944572595	22.34866514837516	22.74800998850297	26.636007918549275
32	25.93764264495168	21.919296215743852	22.719694584190727	29.423366555113738
33	25.784104806051683	21.81311344957295	24.787695492236452	27.61508625213891
34	26.456839756780443	21.824830168598705	23.868421245340773	27.84990882928008
35	25.434311923470297	23.961422702607702	24.400799666073507	26.203465707848494
36	26.440729268120027	22.25493139616912	24.869712525416737	26.434626810294116
37	27.121763561492024	22.20025337404893	24.32830246710165	26.349680597357395
38	26.114858020216225	21.50213219876437	25.19802475645091	27.184985024568498
39	25.572959765275062	20.484242233401925	26.05310114701797	27.88969685430504
40	25.583700091048673	21.394240744402214	26.95992637994879	26.06213278460033
41	23.46346214401312	20.808404793114473	27.86260194155798	27.86553112131442
42	21.77893968574783	21.436713850870575	29.166086933173208	27.618259530208388
43	21.12500030512289	22.184142885388518	28.493107884131412	28.197748925357175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	4.0
4	6.0
5	6.0
6	19.5
7	33.0
8	35.5
9	38.0
10	50.5
11	63.0
12	63.0
13	156.0
14	249.0
15	517.5
16	786.0
17	744.0
18	702.0
19	702.0
20	780.5
21	859.0
22	666.5
23	474.0
24	520.5
25	567.0
26	567.0
27	648.0
28	729.0
29	946.5
30	1164.0
31	1469.0
32	1774.0
33	1774.0
34	2521.0
35	3268.0
36	3763.0
37	4258.0
38	5722.0
39	7186.0
40	7186.0
41	9263.5
42	11341.0
43	14023.5
44	16706.0
45	24788.0
46	32870.0
47	32870.0
48	36821.0
49	40772.0
50	45982.5
51	51193.0
52	51465.0
53	51737.0
54	51737.0
55	46707.5
56	41678.0
57	38980.0
58	36282.0
59	33957.0
60	31632.0
61	31632.0
62	28672.0
63	25712.0
64	20869.5
65	16027.0
66	13646.0
67	11265.0
68	11265.0
69	9522.0
70	7779.0
71	6498.5
72	5218.0
73	4068.5
74	2919.0
75	2919.0
76	2358.5
77	1798.0
78	1437.5
79	1077.0
80	874.0
81	671.0
82	671.0
83	534.0
84	397.0
85	318.5
86	240.0
87	177.0
88	114.0
89	114.0
90	86.5
91	59.0
92	39.0
93	19.0
94	11.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	409671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.69255239659741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.30014634262632	39.43273958594477
2	6.388852569411534	5.838459615639449
3	2.0995032528665334	2.877949871652923
4	1.0775064374149481	1.9693607739701409
5	0.6918888837525912	1.580708453674428
6	0.48713119497893015	1.3354960590355123
7	0.3238626614324809	1.0358678138763586
8	0.2759559283929435	1.0087304573797002
9	0.24353046854204427	1.0014775824622746
>10	1.7122728874415079	15.950752549649053
>50	0.21553041538253118	6.915551814833372
>100	0.16769448777892698	15.251347109377718
>500	0.012362093650369617	3.686296840819948
>1k	0.003762376328373362	2.1152614716843576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1510	0.3685884526852035	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1362	0.3324619023557928	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1313	0.3205010850170015	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1278	0.3119576440607219	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1095	0.2672876527750317	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1053	0.2570355236274962	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1002	0.24458650966263173	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	927	0.22627913618488982	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	919	0.22432634968059736	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	911	0.22237356317630486	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	834	0.2035779930724899	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	796	0.19430225717710065	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	736	0.17965635839490712	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	674	0.1645222629866405	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	655	0.1598843950389459	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	631	0.15402603552606847	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	624	0.1523173473348126	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	614	0.149876364204447	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	603	0.14719128276104484	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	597	0.14572669288282547	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	592	0.1445062013176427	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	586	0.14304161143942334	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	585	0.14279751312638678	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	566	0.13815964517869217	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	541	0.1320571873527782	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	532	0.12986030253544917	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	529	0.1291280075963395	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	525	0.12815161434419328	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	520	0.12693112277901047	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	513	0.12522243458775456	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	496	0.12107276326613306	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	491	0.11985227170095028	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	482	0.11765538688362125	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	482	0.11765538688362125	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	479	0.11692309194451159	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	474	0.11570260037932878	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	469	0.11448210881414599	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	468	0.11423801050110943	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	466	0.1137498138750363	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	465	0.11350571556199975	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	463	0.11301751893592663	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.11277342062289007	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	462	0.11277342062289007	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	461	0.11252932230985352	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	461	0.11252932230985352	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	455	0.11106473243163416	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	454	0.1108206341185976	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	450	0.10984424086645138	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	448	0.10935604424037826	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	447	0.1091119459273417	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	442	0.1078914543621589	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	442	0.1078914543621589	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	431	0.10520637291875676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.440983130365586E-4	0.0	0.0	0.0	0.0
7	2.440983130365586E-4	0.0	0.0	0.0	0.0
8	2.440983130365586E-4	0.0	0.0	0.0	0.0
9	2.440983130365586E-4	0.0	0.0	0.0	0.0
10	2.440983130365586E-4	0.0	0.0	0.0	0.0
11	2.440983130365586E-4	0.0	0.0	0.0	0.0
12	2.440983130365586E-4	0.0	0.0	0.0	0.0
13	2.440983130365586E-4	0.0	0.0	0.0	0.0
14	2.440983130365586E-4	0.0	0.0	0.0	0.0
15	2.440983130365586E-4	0.0	0.0	0.0	0.0
16	2.440983130365586E-4	0.0	0.0	2.440983130365586E-4	0.0
17	2.440983130365586E-4	0.0	0.0	9.763932521462344E-4	0.0
18	4.881966260731172E-4	0.0	0.0	9.763932521462344E-4	0.0
19	4.881966260731172E-4	0.0	0.0	0.0014645898782193517	0.0
20	4.881966260731172E-4	0.0	0.0	0.0024409831303655862	0.0
21	4.881966260731172E-4	0.0	0.0	0.006102457825913965	0.0
22	4.881966260731172E-4	0.0	0.0	0.008543440956279551	0.0
23	4.881966260731172E-4	0.0	0.0	0.010008030834498903	0.0
24	4.881966260731172E-4	0.0	0.0	0.01220491565182793	0.0
25	4.881966260731172E-4	0.0	0.0	0.014889997095230074	0.0
26	4.881966260731172E-4	0.0	0.0	0.01928376672988813	0.0
27	4.881966260731172E-4	0.0	0.0	0.04076441827710529	0.0
28	4.881966260731172E-4	0.0	0.0	0.12107276326613307	0.0
29	4.881966260731172E-4	0.0	0.0	0.24458650966263173	0.0
30	4.881966260731172E-4	0.0	0.0	0.41301434565785716	0.0
31	4.881966260731172E-4	0.0	0.0	0.8804626151228668	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	20	0.0018405538	37.0	3
GTTCTAG	30	3.5954703E-4	30.833334	1
TAGACAT	25	0.005492267	29.599998	5
TGGTCGA	25	0.005492267	29.599998	10
CACTTGC	25	0.005492267	29.599998	1
GGTATCA	675	0.0	28.777777	1
CTAGACA	40	5.9309434E-5	27.75	4
ATTAGAG	35	8.8596134E-4	26.42857	3
ATACGGC	135	0.0	26.037037	29
TATTAGA	40	0.0019290778	23.125	2
TACGGCT	155	0.0	22.677418	30
TGGCAAC	50	2.6979914E-4	22.199999	25
CTTATAC	370	0.0	21.5	37
TCTTCTG	400	0.0	21.274998	16
TTTAAGC	70	5.0887065E-6	21.142857	2
TAATGTT	70	5.0887065E-6	21.142857	31
GCAGTCG	150	0.0	20.966667	9
TAAGTCT	55	5.13533E-4	20.181818	35
GTCTTGG	55	5.13533E-4	20.181818	1
CTCTATG	220	0.0	20.181818	1
>>END_MODULE