FastQCFastQC Report
Fri 10 Feb 2017
ERR1631929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545664
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11840.2169833450621628No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11640.21331808585503165No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10350.1896771639690359No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10250.18784453436547033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8670.15888898662913442No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8620.1579726718273516No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8520.15614004222378608No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8260.15137520525451562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.1438614238798968No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7760.14221205723668778No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7700.14111247947454844No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7270.1332321721792165No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7080.12975017593244195No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6750.12370249824067557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6240.1143560872624912No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6150.11270672061928219No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6030.11050756509500352No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5460.10006157635467981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTATA200.001841088837.011
GGTATCA8200.030.2317071
CGGAATA250.005493845329.5999989
GTATAAA250.005493845329.5999981
AATACTA250.005493845329.59999821
CTATACC358.8633987E-426.4285724
GTACTAT358.8633987E-426.4285721
ATACGGC809.767973E-1025.43750229
CCCCTAT400.001929894223.1250021
TATTAGC603.7216698E-521.5833342
TACGGCT957.1249815E-921.42105330
GTATCAA12050.020.5726152
CATATTT450.003823271520.55555513
CGTTAGA450.003823271520.5555551
TTTTAGC656.893638E-519.92307931
TGTCCGA656.893638E-519.92307912
TAGATGC759.251149E-619.7333345
GTAACGT3700.019.026
GTTCTAG801.6141354E-518.51
CACTCGT609.228341E-418.517
Produced by FastQC (version 0.11.5)