##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631929.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545664
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56824162854797	34.0	31.0	34.0	31.0	34.0
2	32.73468837966221	34.0	31.0	34.0	31.0	34.0
3	32.82926489561342	34.0	31.0	34.0	31.0	34.0
4	36.25931159101572	37.0	37.0	37.0	35.0	37.0
5	36.17638693408398	37.0	35.0	37.0	35.0	37.0
6	36.228389631714755	37.0	37.0	37.0	35.0	37.0
7	36.22313731527094	37.0	36.0	37.0	35.0	37.0
8	36.200471352334034	37.0	36.0	37.0	35.0	37.0
9	38.02752426401595	39.0	38.0	39.0	35.0	39.0
10	37.96080005277973	39.0	38.0	39.0	35.0	39.0
11	38.03425734517945	39.0	38.0	39.0	35.0	39.0
12	37.96796380190007	39.0	38.0	39.0	35.0	39.0
13	38.0217936312456	39.0	38.0	39.0	35.0	39.0
14	39.405603081749945	40.0	39.0	41.0	37.0	41.0
15	39.41371246774572	40.0	39.0	41.0	37.0	41.0
16	39.357954712057236	40.0	39.0	41.0	36.0	41.0
17	39.33116533251231	40.0	39.0	41.0	36.0	41.0
18	39.34915259207131	40.0	39.0	41.0	36.0	41.0
19	39.37473976659629	40.0	39.0	41.0	37.0	41.0
20	39.367046387520524	40.0	39.0	41.0	36.0	41.0
21	39.32820746833216	40.0	39.0	41.0	36.0	41.0
22	39.303362508796624	40.0	39.0	41.0	36.0	41.0
23	39.23956134177809	40.0	39.0	41.0	36.0	41.0
24	39.25718574067558	40.0	39.0	41.0	36.0	41.0
25	39.19744018296974	40.0	39.0	41.0	36.0	41.0
26	39.132671387520524	40.0	39.0	41.0	36.0	41.0
27	39.01735500234577	40.0	39.0	41.0	35.0	41.0
28	39.01368241262022	40.0	39.0	41.0	35.0	41.0
29	38.98381971323012	40.0	39.0	41.0	35.0	41.0
30	38.95026793044804	40.0	38.0	41.0	35.0	41.0
31	38.870279146141215	40.0	38.0	41.0	35.0	41.0
32	38.812465180037535	40.0	38.0	41.0	35.0	41.0
33	38.75062675932442	40.0	38.0	41.0	35.0	41.0
34	38.73024242024396	40.0	38.0	41.0	35.0	41.0
35	38.65194515306123	40.0	38.0	41.0	35.0	41.0
36	38.604260130776446	40.0	38.0	41.0	35.0	41.0
37	38.55716154996481	40.0	38.0	41.0	35.0	41.0
38	38.515197997302366	40.0	38.0	41.0	35.0	41.0
39	38.440839051137694	40.0	38.0	41.0	34.0	41.0
40	38.15207526976308	40.0	38.0	41.0	34.0	41.0
41	38.20853492258973	40.0	37.0	41.0	34.0	41.0
42	38.18643707482993	40.0	37.0	41.0	34.0	41.0
43	37.708879456955195	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	1.0
16	0.0
17	2.0
18	2.0
19	15.0
20	17.0
21	37.0
22	90.0
23	155.0
24	315.0
25	459.0
26	771.0
27	1239.0
28	1844.0
29	2768.0
30	3714.0
31	5225.0
32	7021.0
33	9711.0
34	14176.0
35	20842.0
36	32678.0
37	58297.0
38	132894.0
39	253389.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.967467159277504	16.68829169598874	12.787539584799438	27.55670155993432
2	20.42575650950035	19.851227128782547	33.200834212995545	26.52218214872156
3	21.112626084916723	20.285377081867228	29.5183482875909	29.083648545625145
4	15.394821721792166	16.093969915552428	34.17304421768708	34.33816414496833
5	16.456464051137694	33.31445724841661	33.722767124091014	16.50631157635468
6	33.631502169833446	33.95092950973493	15.861959007741028	16.555609312690596
7	30.00197923997185	28.335019352568615	20.25238974900305	21.410611658456485
8	26.991701853155053	31.222877081867228	20.510057471264368	21.275363593713347
9	28.11968537414966	13.346858139807646	18.14743138634764	40.38602509969505
10	18.921534130893736	24.07177310579404	30.31755805770584	26.68913470560638
11	38.073429802955665	20.024960415200564	19.473155641566972	22.428454140276802
12	22.612266889514427	24.200057178043632	27.239656638517474	25.948019293924467
13	34.10175492610838	18.051951384001878	21.76595853858785	26.0803351513019
14	23.969329110954725	20.350618695754164	23.631575475017595	32.04847671827352
15	28.70777621393385	24.72968713347408	20.711829990616938	25.85070666197514
16	25.150642153413088	24.72950387051372	22.945438951442647	27.174415024630544
17	26.322425521932914	24.356380483227774	22.527599401829697	26.79359459300962
18	25.87801284306826	22.2369443467042	24.344651653764952	27.540391156462583
19	27.43538148017828	23.013612772695286	24.13939713816561	25.41160860896083
20	28.955364473375557	21.58287884119165	23.616364649308	25.845392036124792
21	26.784248182031433	22.906403940886698	22.820270349519117	27.48907752756275
22	27.47643238329815	23.372991437954493	22.23162972085386	26.9189464578935
23	26.99939889749003	22.931694229415907	23.248372624912033	26.82053424818203
24	27.384617640159515	22.44219886230354	23.50090898428337	26.67227451325358
25	27.411374032371572	22.608784893267654	23.75784365470326	26.221997419657516
26	27.21033456486043	23.175983755571195	23.217401184611774	26.396280494956603
27	26.592005336617408	22.359547267182737	24.47165288529205	26.576794510907813
28	25.753210767065447	23.508606028618345	23.073173234811165	27.665009969505043
29	26.570197044334975	23.916366115411684	23.050265364766595	26.463171475486746
30	26.295852392681212	22.94323979591837	24.31184758386113	26.449060227539288
31	27.229027386816796	23.029556650246306	23.538294628196105	26.203121334740793
32	25.011362303542107	22.95276946985691	23.836096938775512	28.199771287825477
33	25.272512022050197	22.600171534130894	25.277093596059114	26.85022284775979
34	25.881128313394324	22.513671416842598	24.570798146844943	27.034402122918134
35	25.45650803424818	23.7994443467042	24.811055887872392	25.932991731175232
36	25.755043396669013	22.799195109078113	25.316311869575415	26.12944962467746
37	26.38271903589022	22.282943349753694	24.842027328172648	26.49231028618344
38	25.44862772695285	22.054047912268356	25.91906374618813	26.578260614590665
39	25.037019117992028	21.470721909453435	26.40159512080694	27.090663851747593
40	24.71319346704199	22.097297970912503	27.218764661036825	25.97074390100868
41	23.312697923997185	21.638040992258972	28.015042223786068	27.034218859957775
42	22.376773985456254	21.97762725779967	28.52103125733052	27.12456749941356
43	21.796013664086324	22.515504046446164	28.25181796856674	27.43666432090077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	11.5
2	15.0
3	25.5
4	36.0
5	36.0
6	52.5
7	69.0
8	73.0
9	77.0
10	124.5
11	172.0
12	172.0
13	357.5
14	543.0
15	1001.0
16	1459.0
17	1440.5
18	1422.0
19	1422.0
20	1700.5
21	1979.0
22	1765.0
23	1551.0
24	1743.5
25	1936.0
26	1936.0
27	2287.0
28	2638.0
29	3652.0
30	4666.0
31	5500.5
32	6335.0
33	6335.0
34	7484.0
35	8633.0
36	9773.0
37	10913.0
38	13561.5
39	16210.0
40	16210.0
41	19136.0
42	22062.0
43	24238.5
44	26415.0
45	33479.5
46	40544.0
47	40544.0
48	44425.5
49	48307.0
50	54152.0
51	59997.0
52	61623.5
53	63250.0
54	63250.0
55	56531.0
56	49812.0
57	46527.0
58	43242.0
59	39628.0
60	36014.0
61	36014.0
62	33188.5
63	30363.0
64	25527.5
65	20692.0
66	17834.0
67	14976.0
68	14976.0
69	12861.5
70	10747.0
71	9273.0
72	7799.0
73	6353.0
74	4907.0
75	4907.0
76	3991.5
77	3076.0
78	2553.5
79	2031.0
80	1609.5
81	1188.0
82	1188.0
83	952.5
84	717.0
85	564.5
86	412.0
87	337.5
88	263.0
89	263.0
90	196.0
91	129.0
92	87.5
93	46.0
94	28.0
95	10.0
96	10.0
97	5.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	545664.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.29300772630254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.96904265484909	44.6090377684431
2	6.24338243087528	6.4048372653029455
3	2.032259696473333	3.127221369391798
4	1.0270628066252678	2.1072456190251136
5	0.6374592449797558	1.6348600988974802
6	0.44855683833940907	1.3804697624777489
7	0.3228435486711997	1.1591731645465144
8	0.25777879867358827	1.057779992963308
9	0.19686888024694893	0.9088197296017755
>10	1.5359461478672614	16.06346887318105
>50	0.2018120485097707	7.180578431881966
>100	0.11871296964144423	11.188343132960295
>500	0.0068349891611740625	2.3648025990272803
>1k	0.0014389450865629604	0.813362192299645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1184	0.2169833450621628	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1164	0.21331808585503165	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1035	0.1896771639690359	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1025	0.18784453436547033	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	867	0.15888898662913442	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	862	0.1579726718273516	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	852	0.15614004222378608	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	826	0.15137520525451562	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	785	0.1438614238798968	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	776	0.14221205723668778	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	770	0.14111247947454844	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	727	0.1332321721792165	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	708	0.12975017593244195	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	675	0.12370249824067557	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	624	0.1143560872624912	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	615	0.11270672061928219	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	603	0.11050756509500352	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	546	0.10006157635467981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.665259207131128E-4	0.0	0.0	0.0	0.0
9	3.665259207131128E-4	0.0	0.0	0.0	1.832629603565564E-4
10	3.665259207131128E-4	0.0	0.0	0.0	1.832629603565564E-4
11	3.665259207131128E-4	0.0	0.0	0.0	1.832629603565564E-4
12	3.665259207131128E-4	0.0	0.0	0.0	1.832629603565564E-4
13	3.665259207131128E-4	0.0	0.0	0.0	1.832629603565564E-4
14	3.665259207131128E-4	0.0	0.0	0.0	1.832629603565564E-4
15	3.665259207131128E-4	0.0	0.0	1.832629603565564E-4	1.832629603565564E-4
16	3.665259207131128E-4	0.0	0.0	3.665259207131128E-4	1.832629603565564E-4
17	3.665259207131128E-4	0.0	0.0	5.497888810696692E-4	1.832629603565564E-4
18	3.665259207131128E-4	0.0	0.0	9.163148017827821E-4	3.665259207131128E-4
19	3.665259207131128E-4	0.0	0.0	0.0014661036828524513	3.665259207131128E-4
20	3.665259207131128E-4	0.0	0.0	0.0018326296035655642	3.665259207131128E-4
21	5.497888810696692E-4	0.0	0.0	0.0036652592071311284	3.665259207131128E-4
22	5.497888810696692E-4	0.0	0.0	0.005864414731409805	3.665259207131128E-4
23	5.497888810696692E-4	0.0	0.0	0.008246833216045038	3.665259207131128E-4
24	5.497888810696692E-4	0.0	0.0	0.010262725779967159	3.665259207131128E-4
25	5.497888810696692E-4	0.0	0.0	0.010629251700680272	3.665259207131128E-4
26	5.497888810696692E-4	0.0	0.0	0.013011670185315505	3.665259207131128E-4
27	5.497888810696692E-4	0.0	0.0	0.029871862538118694	3.665259207131128E-4
28	5.497888810696692E-4	0.0	0.0	0.09694610602861835	3.665259207131128E-4
29	5.497888810696692E-4	0.0	0.0	0.20708714520290875	3.665259207131128E-4
30	5.497888810696692E-4	0.0	0.0	0.3516816209242318	3.665259207131128E-4
31	5.497888810696692E-4	0.0	0.0	0.7830826296035656	3.665259207131128E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTATA	20	0.0018410888	37.0	11
GGTATCA	820	0.0	30.231707	1
CGGAATA	25	0.0054938453	29.599998	9
GTATAAA	25	0.0054938453	29.599998	1
AATACTA	25	0.0054938453	29.599998	21
CTATACC	35	8.8633987E-4	26.428572	4
GTACTAT	35	8.8633987E-4	26.428572	1
ATACGGC	80	9.767973E-10	25.437502	29
CCCCTAT	40	0.0019298942	23.125002	1
TATTAGC	60	3.7216698E-5	21.583334	2
TACGGCT	95	7.1249815E-9	21.421053	30
GTATCAA	1205	0.0	20.572615	2
CATATTT	45	0.0038232715	20.555555	13
CGTTAGA	45	0.0038232715	20.555555	1
TTTTAGC	65	6.893638E-5	19.923079	31
TGTCCGA	65	6.893638E-5	19.923079	12
TAGATGC	75	9.251149E-6	19.733334	5
GTAACGT	370	0.0	19.0	26
GTTCTAG	80	1.6141354E-5	18.5	1
CACTCGT	60	9.228341E-4	18.5	17
>>END_MODULE