FastQCFastQC Report
Fri 10 Feb 2017
ERR1631928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403411
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18340.45462320065640255No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16230.40231922282733984No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15010.372077112423806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14970.3710855678204114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.3522462203559149No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13660.3386124820592398No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12160.3014295594319441No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12070.2991985840743064No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11330.2808550089115071No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11200.2776324889504748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10740.26622972601143746No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10690.26499029525719425No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10590.2625114337487079No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10480.2597846860893729No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9430.23375664025026585No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9300.23053412028923356No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8830.21888347119934756No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8830.21888347119934756No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7570.18764981619241913No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7500.18591461313647867No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7410.18368363777884095No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7270.18021323166696No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7220.1789738009127168No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT7090.17575128095168452No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7000.17352030559404677No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6810.16881046872792263No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6790.16831469642622535No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6740.16707526567198216No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6710.16633160721943627No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6670.1653400626160417No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6590.1633569734092526No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6450.15988656729737166No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6280.1556725027329448No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6200.1536894135261557No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6150.15244998277191252No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6110.15145843816851795No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6020.14922746281088023No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5890.1460049428498479No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5890.1460049428498479No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5850.14501339824645337No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5840.14476551209560473No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5730.1420387644362697No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5720.14179087828542108No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5600.1388162444752374No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5570.1380725860226915No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5490.1360894968159024No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG5470.13559372451420512No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT5460.13534583836335648No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5390.13361063530741601No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC5240.12989234304468644No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC5180.1284050261395946No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5180.1284050261395946No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5160.12790925383789734No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4950.12270364467007593No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4950.12270364467007593No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA4690.11625860474801133No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4680.11601071859716271No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4670.11576283244631405No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT4580.11353185708867632No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4510.11179665403273584No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4410.10931779252424947No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4390.10882202022255219No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4270.10584738641236853No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4240.10510372795982262No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG4230.10485584180897398No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4210.10436006950727669No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4190.10386429720557941No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4180.10361641105473078No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4110.10188120799879033No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4060.10064177724454712No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4050.10039389109369849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCT200.001840520137.031
TCAATGC352.3844947E-531.71428732
TATGCGG303.5953746E-430.8333325
CGCTTCT303.5953746E-430.83333231
GGTATCA9150.029.721311
TTGTCGG451.3211335E-424.66666622
TCGGGTA451.3211335E-424.66666625
CAATGCT451.3211335E-424.66666633
TGTCGGG451.3211335E-424.66666623
TAAAGAC952.8012437E-1023.36842237
GTATTAG753.728328E-722.21
TAGCGAA502.6978925E-422.19999910
ATACGGC603.7185746E-521.58333229
GACGGCT450.003821569520.55555536
CTTATAC4000.020.34999837
TGTTACG555.135144E-420.18181816
TCCTTGT555.135144E-420.18181819
TTCTTCT5050.020.14851615
TATTAGC656.887944E-519.9230772
TTCTGCG5200.019.92307718
Produced by FastQC (version 0.11.5)