##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631928.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403411
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49249028906004	33.0	31.0	34.0	31.0	34.0
2	32.657728718354235	34.0	31.0	34.0	31.0	34.0
3	32.739174192077066	34.0	31.0	34.0	31.0	34.0
4	36.18674503174182	37.0	35.0	37.0	35.0	37.0
5	36.112111469444315	37.0	35.0	37.0	35.0	37.0
6	36.17077868476566	37.0	36.0	37.0	35.0	37.0
7	36.18524532052919	37.0	36.0	37.0	35.0	37.0
8	36.16404113918559	37.0	36.0	37.0	35.0	37.0
9	37.9870603429257	39.0	38.0	39.0	35.0	39.0
10	37.91052797271269	39.0	38.0	39.0	35.0	39.0
11	37.99801691079321	39.0	38.0	39.0	35.0	39.0
12	37.9124292594897	39.0	38.0	39.0	35.0	39.0
13	37.97499076624088	39.0	38.0	39.0	35.0	39.0
14	39.292577544985136	40.0	39.0	41.0	36.0	41.0
15	39.31433699130663	40.0	39.0	41.0	36.0	41.0
16	39.2441083659097	40.0	39.0	41.0	36.0	41.0
17	39.23402683615469	40.0	39.0	41.0	36.0	41.0
18	39.28281578836472	40.0	39.0	41.0	36.0	41.0
19	39.28952110874518	40.0	39.0	41.0	36.0	41.0
20	39.299808384005395	40.0	39.0	41.0	36.0	41.0
21	39.24540728934015	40.0	39.0	41.0	36.0	41.0
22	39.210331398995066	40.0	39.0	41.0	36.0	41.0
23	39.14496382101628	40.0	39.0	41.0	36.0	41.0
24	39.151136186172415	40.0	39.0	41.0	36.0	41.0
25	39.09276891309359	40.0	39.0	41.0	36.0	41.0
26	39.0139336805392	40.0	39.0	41.0	35.0	41.0
27	38.85341500355717	40.0	38.0	41.0	35.0	41.0
28	38.863960080414266	40.0	38.0	41.0	35.0	41.0
29	38.824722181596435	40.0	38.0	41.0	35.0	41.0
30	38.778149331574994	40.0	38.0	41.0	35.0	41.0
31	38.66531155570869	40.0	38.0	41.0	35.0	41.0
32	38.60598248436458	40.0	38.0	41.0	35.0	41.0
33	38.5212797866196	40.0	38.0	41.0	35.0	41.0
34	38.48305822102025	40.0	38.0	41.0	35.0	41.0
35	38.37957814734848	40.0	38.0	41.0	34.0	41.0
36	38.30657815478507	40.0	38.0	41.0	34.0	41.0
37	38.23099766739132	40.0	38.0	41.0	34.0	41.0
38	38.173277377166215	40.0	37.0	41.0	34.0	41.0
39	38.06042472813086	40.0	37.0	41.0	34.0	41.0
40	37.74127130891324	40.0	37.0	41.0	33.0	41.0
41	37.771391955102864	40.0	37.0	41.0	33.0	41.0
42	37.72330699956124	40.0	36.0	41.0	33.0	41.0
43	37.20682380996056	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	2.0
18	2.0
19	5.0
20	21.0
21	38.0
22	69.0
23	149.0
24	245.0
25	389.0
26	690.0
27	1058.0
28	1652.0
29	2378.0
30	3318.0
31	4481.0
32	5865.0
33	7914.0
34	11311.0
35	17168.0
36	26101.0
37	48273.0
38	104201.0
39	168079.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.21126592978377	16.41229416153749	13.231171187696914	27.14526872098183
2	21.288462634881054	20.489773456846738	31.91608558021472	26.305678328057493
3	21.98427906031318	19.904266368542256	29.998934089551348	28.11252048159321
4	14.923985711842267	17.354757307064013	35.69510994990221	32.02614703119151
5	16.54069918767708	32.568521929248334	33.97577160761605	16.915007275458528
6	30.51924712018264	34.976487998592006	16.864438500685406	17.639826380539947
7	28.610275872497283	28.56069864232755	20.515801502685846	22.313223982489323
8	26.80814355582768	29.39706651529086	21.37249604001874	22.422293888862725
9	28.624901155397346	12.618892395100778	18.453884499926872	40.302321949574996
10	19.984581481417216	22.883114243290343	28.761238538364097	28.37106573692834
11	38.69378871671819	19.851466618411497	18.826457384652375	22.62828728021794
12	21.624100483130107	24.841414834994584	27.13956733951231	26.394917342363
13	36.34432377897479	17.525055092697027	20.820453582078823	25.310167546249357
14	23.34269516696372	21.575019025262076	24.50528121444383	30.577004593330376
15	30.442898185721262	23.943323310469967	20.703203432727417	24.910575071081354
16	23.40243572931824	24.716975987268565	24.168899707742227	27.71168857567097
17	26.308652961867672	24.07594240117399	22.10053766506119	27.514866971897145
18	26.39466945621215	20.523981745663853	24.262352786612166	28.81899601151183
19	27.473965757007125	22.626056304860303	24.770271509701026	25.129706428431547
20	30.43422217044156	19.97664912459006	23.684778055134835	25.904350649833546
21	27.303667971374107	21.990228327933547	22.922280255124427	27.78382344556792
22	28.03270114101995	22.89079871396665	22.288435367404457	26.788064777608938
23	27.939495948300863	21.56411203462474	23.54050831534093	26.95588370173347
24	27.442979988151038	21.046029979351083	24.426701304624814	27.084288727873062
25	27.45487852339178	22.044763281120247	24.458926504235134	26.041431691252843
26	27.529988027098913	23.078944302460766	23.358559880618028	26.03250778982229
27	26.323278244767746	21.716314131245802	26.029037383710406	25.931370240276046
28	25.673568643393462	23.425984913648858	22.726450195954	28.173996247003675
29	26.463333919997222	23.97133444551586	23.024161463123217	26.5411701713637
30	26.35475978592552	21.935693374746844	25.207542679798024	26.502004159529612
31	27.89066237658368	22.56829883171257	23.32980508711959	26.21123370458416
32	24.908344095723717	22.47558941129518	23.93910924590554	28.676957247075563
33	24.99312115931395	22.096571486647612	25.923933655750588	26.986373698287853
34	25.48393573799426	22.60845638815005	24.540233161713488	27.367374712142205
35	25.006011239158077	24.624514453002025	25.230843977977795	25.1386303298621
36	25.93459276023708	22.848410182171534	25.50698915002318	25.71000790756821
37	26.897630456284038	23.139924295569532	24.50701641749977	25.45542883064666
38	25.791314565046562	22.469640143674813	25.123757160811184	26.61528813046744
39	25.370403880905577	21.23615865705199	26.197599966287484	27.195837495754947
40	25.306449253986628	22.57821427774652	26.623220487294596	25.492115980972258
41	23.075473896348885	21.997169140157308	27.614269318387453	27.313087645106354
42	21.286231659523413	22.813706121052725	28.601599857217575	27.298462362206287
43	20.916633408608096	23.93340786443602	27.5146190857463	27.635339641209583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	4.0
2	6.0
3	29.0
4	52.0
5	52.0
6	76.5
7	101.0
8	103.0
9	105.0
10	169.0
11	233.0
12	233.0
13	552.5
14	872.0
15	1659.5
16	2447.0
17	2318.0
18	2189.0
19	2189.0
20	2494.5
21	2800.0
22	2076.5
23	1353.0
24	1252.5
25	1152.0
26	1152.0
27	1157.5
28	1163.0
29	1248.5
30	1334.0
31	1511.0
32	1688.0
33	1688.0
34	2480.0
35	3272.0
36	3501.0
37	3730.0
38	5076.5
39	6423.0
40	6423.0
41	8776.0
42	11129.0
43	13886.0
44	16643.0
45	25774.5
46	34906.0
47	34906.0
48	38776.5
49	42647.0
50	48633.5
51	54620.0
52	53357.0
53	52094.0
54	52094.0
55	45408.5
56	38723.0
57	35572.5
58	32422.0
59	30220.5
60	28019.0
61	28019.0
62	25635.5
63	23252.0
64	18212.0
65	13172.0
66	11359.0
67	9546.0
68	9546.0
69	8048.5
70	6551.0
71	5436.5
72	4322.0
73	3396.0
74	2470.0
75	2470.0
76	2033.5
77	1597.0
78	1267.5
79	938.0
80	754.5
81	571.0
82	571.0
83	459.0
84	347.0
85	295.5
86	244.0
87	191.5
88	139.0
89	139.0
90	120.0
91	101.0
92	63.5
93	26.0
94	17.5
95	9.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	403411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.15523966284553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.42807379492339	27.811953757494805
2	7.788194248688239	5.32015282209484
3	2.881568433865228	2.9526198139067197
4	1.6045643910786656	2.192171253270385
5	1.057622285938495	1.8061671324497923
6	0.7321011361009104	1.5003053856580855
7	0.5248854400890381	1.2549311601245476
8	0.411679113075544	1.1248799017026314
9	0.35583962805498703	1.0938408999980305
>10	2.5719434050578545	17.827216629861713
>50	0.33408385671381013	8.059281000909342
>100	0.2710354932992733	18.281647280287565
>500	0.028263059461688927	6.277872973773453
>1k	0.010145713652913974	4.496959988468087
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1834	0.45462320065640255	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1623	0.40231922282733984	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1501	0.372077112423806	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1497	0.3710855678204114	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1421	0.3522462203559149	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1366	0.3386124820592398	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1216	0.3014295594319441	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1207	0.2991985840743064	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1133	0.2808550089115071	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1120	0.2776324889504748	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1074	0.26622972601143746	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1069	0.26499029525719425	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1059	0.2625114337487079	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1048	0.2597846860893729	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	943	0.23375664025026585	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	930	0.23053412028923356	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	883	0.21888347119934756	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	883	0.21888347119934756	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	757	0.18764981619241913	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	750	0.18591461313647867	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	741	0.18368363777884095	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	727	0.18021323166696	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	722	0.1789738009127168	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	709	0.17575128095168452	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	700	0.17352030559404677	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	681	0.16881046872792263	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	679	0.16831469642622535	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	674	0.16707526567198216	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	671	0.16633160721943627	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	667	0.1653400626160417	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	659	0.1633569734092526	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	645	0.15988656729737166	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	628	0.1556725027329448	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	620	0.1536894135261557	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	615	0.15244998277191252	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	611	0.15145843816851795	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	602	0.14922746281088023	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	589	0.1460049428498479	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	589	0.1460049428498479	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	585	0.14501339824645337	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	584	0.14476551209560473	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	573	0.1420387644362697	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	572	0.14179087828542108	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	560	0.1388162444752374	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	557	0.1380725860226915	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	549	0.1360894968159024	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	547	0.13559372451420512	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	546	0.13534583836335648	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	539	0.13361063530741601	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	524	0.12989234304468644	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	518	0.1284050261395946	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	518	0.1284050261395946	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	516	0.12790925383789734	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	495	0.12270364467007593	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	495	0.12270364467007593	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	469	0.11625860474801133	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	468	0.11601071859716271	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	467	0.11576283244631405	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	458	0.11353185708867632	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	451	0.11179665403273584	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	441	0.10931779252424947	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	439	0.10882202022255219	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	427	0.10584738641236853	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	424	0.10510372795982262	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	423	0.10485584180897398	No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG	421	0.10436006950727669	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	419	0.10386429720557941	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	418	0.10361641105473078	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	411	0.10188120799879033	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	406	0.10064177724454712	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	405	0.10039389109369849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.478861508486382E-4	0.0
11	0.0	0.0	0.0	2.478861508486382E-4	0.0
12	0.0	0.0	0.0	2.478861508486382E-4	0.0
13	0.0	0.0	0.0	2.478861508486382E-4	0.0
14	0.0	0.0	0.0	2.478861508486382E-4	0.0
15	0.0	0.0	0.0	2.478861508486382E-4	0.0
16	0.0	0.0	0.0	7.436584525459147E-4	0.0
17	0.0	0.0	0.0	0.0019830892067891057	0.0
18	0.0	0.0	0.0	0.0019830892067891057	0.0
19	0.0	0.0	0.0	0.002230975357637744	2.478861508486382E-4
20	0.0	0.0	0.0	0.0027267476593350205	2.478861508486382E-4
21	2.478861508486382E-4	0.0	0.0	0.003470406111880935	2.478861508486382E-4
22	2.478861508486382E-4	0.0	0.0	0.0064450399220645944	2.478861508486382E-4
23	2.478861508486382E-4	0.0	0.0	0.008180242978005061	2.478861508486382E-4
24	2.478861508486382E-4	0.0	0.0	0.009419673732248253	2.478861508486382E-4
25	2.478861508486382E-4	0.0	0.0	0.010659104486491443	2.478861508486382E-4
26	2.478861508486382E-4	0.0	0.0	0.012394307542431912	2.478861508486382E-4
27	2.478861508486382E-4	0.0	0.0	0.027515362744198843	2.478861508486382E-4
28	2.478861508486382E-4	0.0	0.0	0.09518828192587708	2.478861508486382E-4
29	2.478861508486382E-4	0.0	0.0	0.20896802516540203	2.478861508486382E-4
30	2.478861508486382E-4	0.0	0.0	0.3643926417474982	2.478861508486382E-4
31	2.478861508486382E-4	0.0	0.0	0.8200073870072953	2.478861508486382E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGCT	20	0.0018405201	37.0	31
TCAATGC	35	2.3844947E-5	31.714287	32
TATGCGG	30	3.5953746E-4	30.833332	5
CGCTTCT	30	3.5953746E-4	30.833332	31
GGTATCA	915	0.0	29.72131	1
TTGTCGG	45	1.3211335E-4	24.666666	22
TCGGGTA	45	1.3211335E-4	24.666666	25
CAATGCT	45	1.3211335E-4	24.666666	33
TGTCGGG	45	1.3211335E-4	24.666666	23
TAAAGAC	95	2.8012437E-10	23.368422	37
GTATTAG	75	3.728328E-7	22.2	1
TAGCGAA	50	2.6978925E-4	22.199999	10
ATACGGC	60	3.7185746E-5	21.583332	29
GACGGCT	45	0.0038215695	20.555555	36
CTTATAC	400	0.0	20.349998	37
TGTTACG	55	5.135144E-4	20.181818	16
TCCTTGT	55	5.135144E-4	20.181818	19
TTCTTCT	505	0.0	20.148516	15
TATTAGC	65	6.887944E-5	19.923077	2
TTCTGCG	520	0.0	19.923077	18
>>END_MODULE