FastQCFastQC Report
Fri 10 Feb 2017
ERR1631927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210300
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10720.5097479790775082No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10690.5083214455539705No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9970.4740846409890633No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9200.4374702805515929No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8540.4060865430337613No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8280.39372325249643364No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7930.37708036138849266No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7820.371849738468854No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7060.3357108892058963No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6800.3233475986685687No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6510.3095577746077033No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6470.3076557299096529No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5680.2700903471231574No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5120.24346172135045174No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4960.2358535425582501No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4700.22349025202092251No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4700.22349025202092251No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4430.21065145030908228No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4390.20874940561103186No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4370.20779838326200664No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4320.20542082738944367No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4180.19876367094626723No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4110.19543509272467902No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4110.19543509272467902No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4080.19400855920114124No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4040.19210651450309082No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3940.1873514027579648No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3820.18164526866381359No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3790.1802187351402758No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3780.1797432239657632No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3710.17641464574417498No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3670.1745126010461246No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3640.17308606752258676No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3620.1721350451735616No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3590.17070851165002376No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3560.169281978126486No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3550.16880646695197338No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3550.16880646695197338No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3540.16833095577746077No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3520.16737993342843557No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3500.16642891107941038No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3480.16547788873038516No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3450.16405135520684735No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3440.16357584403233477No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3290.15644317641464575No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3280.15596766524013314No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3260.15501664289110795No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3250.15454113171659534No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3220.15311459819305753No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3200.15216357584403234No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3170.15073704232049454No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3130.14883499762244412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.14788397527341893No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT3100.14740846409890634No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3090.14693295292439373No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3000.14265335235378032No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2930.1393247741321921No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2880.1369472182596291No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC2830.1345696623870661No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2800.13314312886352828No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2750.13076557299096528No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG2740.13029006181645267No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT2710.1288635282929149No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2680.12743699476937706No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2620.12458392772230148No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2560.12173086067522587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2560.12173086067522587No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2540.12077983832620068No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2530.12030432715168805No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2460.11697574893009986No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG2440.11602472658107466No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2380.11317165953399905No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2350.11174512601046126No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC2350.11174512601046126No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2310.10984308131241084No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2290.10889205896338565No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2250.10699001426533523No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2220.10556348074179742No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2210.10508796956728483No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2190.10413694721825963No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2170.10318592486923443No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2160.10271041369472184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC658.54925E-1131.30769237
CAAGACC250.00548626429.64
ATACGGC556.220944E-726.9090929
GGTATCA5300.026.5283031
TACGGCT701.9074287E-723.78571330
GGGATGG400.00192597323.12500218
TAGGACC652.665578E-622.769234
TTAGGAC652.665578E-622.769233
TATACAC502.691943E-422.237
GGACCGT753.7107748E-722.26
TTTACCG502.691943E-422.230
CTTAGGA603.7076847E-521.5833322
CTTATAC1700.020.67647237
TTACCGG450.003815577420.55555531
TAGCACC450.003815577420.55555534
AAGGTAG450.003815577420.5555556
TAAGGTA450.003815577420.5555555
CCTTATT1101.7298589E-920.18181827
GCCGTTT555.123915E-420.18181826
TACCCGA555.123915E-420.18181811
Produced by FastQC (version 0.11.5)