FastQCFastQC Report
Fri 10 Feb 2017
ERR1631926.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631926.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388438
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9230.23761835865698No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8910.22938023571329272No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8780.22603349826741978No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7850.20209145346232862No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7750.19951704004242632No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7000.18020893939315927No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT6650.17119849242350133No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6400.16476245887374563No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6280.16167316276986288No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5990.15420736385214628No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5850.1506031850642831No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5520.14210762077860561No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5510.14185017943661538No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5490.1413352967526349No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG5170.13309717380894762No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4560.11739325194754374No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT4230.10889768766186624No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4190.10786792229390535No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC4120.10606583289997373No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3980.10246165411211056No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3890.10014468203419852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAA303.5951304E-430.83333219
TATATTC303.5951304E-430.8333323
TACTCTC303.5951304E-430.83333232
CTGTACT250.005491919329.64
GTTAGAC250.005491919329.62
TACTGGA250.005491919329.62
GGTCTAT250.005491919329.61
ATACGGC556.2438266E-726.9090929
TGCACGA358.858782E-426.4285710
GGTATCA5750.026.060871
TGTTTAC601.3336012E-624.66666614
ATCTTAC601.3336012E-624.6666661
CTTGTCG400.001928898323.12521
GTAAGTT400.001928898323.12529
TACGGCT652.6752568E-622.7692330
CTGTTTA652.6752568E-622.7692313
CTGCACG502.6976413E-422.29
TTCTGCG2250.022.218
CCGTTTA502.6976413E-422.227
AACGTCA2100.022.02381128
Produced by FastQC (version 0.11.5)