##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631926.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388438
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19167280235198	33.0	31.0	34.0	30.0	34.0
2	32.38413080079704	34.0	31.0	34.0	31.0	34.0
3	32.47398812680531	34.0	31.0	34.0	30.0	34.0
4	36.025538181125434	37.0	35.0	37.0	35.0	37.0
5	35.86611505568456	37.0	35.0	37.0	35.0	37.0
6	35.89909844042035	37.0	35.0	37.0	35.0	37.0
7	35.90055813282944	37.0	35.0	37.0	35.0	37.0
8	35.87379710532955	37.0	35.0	37.0	35.0	37.0
9	37.671329787507915	39.0	37.0	39.0	35.0	39.0
10	37.545803963566904	39.0	37.0	39.0	35.0	39.0
11	37.64069941663792	39.0	37.0	39.0	35.0	39.0
12	37.55009036191104	39.0	37.0	39.0	35.0	39.0
13	37.61625793562936	39.0	37.0	39.0	35.0	39.0
14	38.89280399960869	40.0	38.0	41.0	36.0	41.0
15	38.883945443030804	40.0	38.0	41.0	36.0	41.0
16	38.83187020837302	40.0	38.0	41.0	36.0	41.0
17	38.78781942034507	40.0	38.0	41.0	35.0	41.0
18	38.78254702166112	40.0	38.0	41.0	35.0	41.0
19	38.79936566453333	40.0	38.0	41.0	35.0	41.0
20	38.77528460140357	40.0	38.0	41.0	35.0	41.0
21	38.74357297689721	40.0	38.0	41.0	35.0	41.0
22	38.69664399466581	40.0	38.0	41.0	35.0	41.0
23	38.63545533650158	40.0	38.0	41.0	35.0	41.0
24	38.6427795426812	40.0	38.0	41.0	35.0	41.0
25	38.57259588402783	40.0	38.0	41.0	35.0	41.0
26	38.474343395857254	40.0	38.0	41.0	34.0	41.0
27	38.357266796760356	40.0	38.0	41.0	34.0	41.0
28	38.34407292798336	40.0	38.0	41.0	34.0	41.0
29	38.30762953161122	40.0	38.0	41.0	34.0	41.0
30	38.2649792244837	40.0	38.0	41.0	34.0	41.0
31	38.19125317296454	40.0	38.0	41.0	34.0	41.0
32	38.14752418661408	40.0	38.0	41.0	34.0	41.0
33	38.070724285471556	40.0	37.0	41.0	33.0	41.0
34	38.023785005586475	40.0	37.0	41.0	33.0	41.0
35	37.92481168165833	40.0	37.0	41.0	33.0	41.0
36	37.89238951904809	40.0	37.0	41.0	33.0	41.0
37	37.84189497423012	40.0	37.0	41.0	33.0	41.0
38	37.80944706748567	40.0	37.0	41.0	33.0	41.0
39	37.7333654276873	40.0	37.0	41.0	33.0	41.0
40	37.41058032427311	39.0	36.0	41.0	32.0	41.0
41	37.47610944346331	39.0	36.0	41.0	32.0	41.0
42	37.457810512874644	39.0	36.0	41.0	33.0	41.0
43	36.934481178463486	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	3.0
19	9.0
20	20.0
21	45.0
22	89.0
23	192.0
24	332.0
25	554.0
26	907.0
27	1383.0
28	1972.0
29	2773.0
30	4019.0
31	5333.0
32	7257.0
33	9817.0
34	13993.0
35	21333.0
36	32309.0
37	56244.0
38	118581.0
39	111270.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.36470685154388	16.035506309887293	12.133982772025394	28.465804066543438
2	21.580535375014804	18.62047482481117	31.891576004407398	27.907413795766633
3	21.963093209212282	19.23807660424572	28.611515866109904	30.18731432043209
4	16.34829754040542	14.97072891941571	33.363110715223534	35.317862824955334
5	17.568569501439097	32.334632553972575	32.891735618039434	17.205062326548894
6	35.85977684984476	32.74447917042102	14.805966460541967	16.589777519192253
7	31.302035331249773	26.906224416766637	19.465397309223093	22.32634294276049
8	28.57830593299317	29.952012933853023	19.594117980218208	21.875563152935605
9	28.926366627363954	12.269654359254243	16.78749246984075	42.016486543541056
10	19.91437500965405	22.667452720897543	29.313300964375266	28.104871305073136
11	40.12506500393885	18.6802012161529	18.312574979790856	22.882158800117395
12	24.306324304007333	22.76939949232567	25.68131851157714	27.24295769208986
13	34.94663240980543	16.962037699710123	20.303111436059293	27.788218454425156
14	24.21570495162677	19.11965358693022	22.735932117866945	33.92870934357607
15	30.195809884717768	23.040742666783373	19.634536270910672	27.12891117758819
16	26.287077989280146	23.09351814189137	21.307390111163173	29.312013757665316
17	27.43861311200243	23.230991818514152	21.16785690380447	28.162538165678953
18	26.90905627152853	20.60380292350388	22.938795895355245	29.548344909612346
19	28.799705487104767	21.330817273284282	22.341789423279906	27.52768781633105
20	29.578722987967193	20.42256421874276	22.349770104881603	27.648942688408447
21	29.05380009164912	21.116883518090404	21.229900267224114	28.599416123036363
22	29.71259248580211	21.996818025013003	20.497479649261916	27.793109839922973
23	29.310726550955362	21.684541677178853	21.012362333242372	27.99236943862341
24	29.2623275786612	20.848372198394596	21.41680268150902	28.47249754143518
25	28.577791050309187	20.96447824363219	22.491620284318216	27.96611042174041
26	29.117645544462693	21.687116090598757	21.93709163367127	27.25814673126728
27	28.75825743104434	20.84090639947688	22.757557190594124	27.643278978884663
28	27.04447041741539	21.674244023499245	21.918040974363993	29.36324458472137
29	28.468893362647318	22.070961131506188	22.02693866202586	27.433206843820635
30	27.69399492325674	20.77551629861136	23.293292623275786	28.237196154856115
31	28.23899824425005	21.545265911162144	22.361869847955145	27.853865996632663
32	26.424294224560935	21.468805832591045	22.47926309990269	29.627636842945336
33	27.24295769208986	21.62327063778518	23.33113650054835	27.80263516957661
34	27.421107100747093	21.641291531724498	22.940855426091165	27.996745941437247
35	27.11037540096489	22.78896503431693	23.47684830011482	26.623811264603358
36	27.35983606135342	21.62121110704926	23.299986098167533	27.718966733429788
37	26.934542964385567	21.592377676746352	23.66915698258152	27.80392237628656
38	27.195331043821664	20.788645807052863	24.43942147781628	27.57660167130919
39	25.65711902543006	20.398364732595677	25.500852130841988	28.44366411113228
40	26.03427059144574	20.83189595250722	26.392114056812154	26.74171939923489
41	24.03292159881371	20.060086809220522	27.377599513950745	28.529392078015025
42	22.749833950334416	20.950318969822725	28.020173103558356	28.279673976284503
43	22.292618126959773	20.57908855467282	28.11928802022459	29.00900529814282
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	5.0
2	2.0
3	4.5
4	7.0
5	7.0
6	11.5
7	16.0
8	20.0
9	24.0
10	34.5
11	45.0
12	45.0
13	97.0
14	149.0
15	313.0
16	477.0
17	483.5
18	490.0
19	490.0
20	552.0
21	614.0
22	507.5
23	401.0
24	452.0
25	503.0
26	503.0
27	575.5
28	648.0
29	904.0
30	1160.0
31	1547.0
32	1934.0
33	1934.0
34	2713.0
35	3492.0
36	4237.0
37	4982.0
38	6501.5
39	8021.0
40	8021.0
41	9507.5
42	10994.0
43	13293.0
44	15592.0
45	20443.5
46	25295.0
47	25295.0
48	27648.0
49	30001.0
50	35143.5
51	40286.0
52	41958.5
53	43631.0
54	43631.0
55	41574.0
56	39517.0
57	37370.5
58	35224.0
59	32904.5
60	30585.0
61	30585.0
62	29466.5
63	28348.0
64	22817.0
65	17286.0
66	15228.0
67	13170.0
68	13170.0
69	11354.0
70	9538.0
71	8766.5
72	7995.0
73	8237.0
74	8479.0
75	8479.0
76	7115.5
77	5752.0
78	3928.0
79	2104.0
80	1431.5
81	759.0
82	759.0
83	604.0
84	449.0
85	337.0
86	225.0
87	179.0
88	133.0
89	133.0
90	104.0
91	75.0
92	48.5
93	22.0
94	13.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	388438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.28772468290493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5595465618494	39.60356735249071
2	6.523864271660772	6.039496665505478
3	2.2618661050201396	3.1408990661630027
4	1.1619519469948587	2.1513644726905343
5	0.7316815903702922	1.6933938005305054
6	0.5022955019811381	1.395006954309867
7	0.41920352193246957	1.3582784046519973
8	0.30373920796376014	1.1247517466904107
9	0.24436006807922386	1.0179784399272318
>10	1.8511556826140603	17.226284080105323
>50	0.25905381922514636	8.500928150534135
>100	0.17288904997963334	14.096561029538538
>500	0.008392672329108414	2.65148983686231
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	923	0.23761835865698	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	891	0.22938023571329272	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	878	0.22603349826741978	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	785	0.20209145346232862	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	775	0.19951704004242632	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	700	0.18020893939315927	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	665	0.17119849242350133	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	640	0.16476245887374563	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	628	0.16167316276986288	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	599	0.15420736385214628	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	585	0.1506031850642831	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	552	0.14210762077860561	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	551	0.14185017943661538	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	549	0.1413352967526349	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	517	0.13309717380894762	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	456	0.11739325194754374	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	423	0.10889768766186624	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	419	0.10786792229390535	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	412	0.10606583289997373	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	398	0.10246165411211056	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	389	0.10014468203419852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.148826839804551E-4	0.0	0.0	0.0	0.0
9	5.148826839804551E-4	0.0	0.0	0.0	0.0
10	5.148826839804551E-4	0.0	0.0	0.0	0.0
11	5.148826839804551E-4	0.0	0.0	0.0	0.0
12	5.148826839804551E-4	0.0	0.0	0.0	0.0
13	5.148826839804551E-4	0.0	0.0	0.0	0.0
14	7.723240259706825E-4	0.0	0.0	0.0	0.0
15	7.723240259706825E-4	0.0	0.0	0.0	0.0
16	7.723240259706825E-4	0.0	0.0	2.5744134199022755E-4	0.0
17	7.723240259706825E-4	0.0	0.0	7.723240259706825E-4	0.0
18	7.723240259706825E-4	0.0	0.0	0.001544648051941365	0.0
19	7.723240259706825E-4	0.0	0.0	0.0018020893939315926	0.0
20	0.0010297653679609102	0.0	0.0	0.002574413419902275	0.0
21	0.0010297653679609102	0.0	0.0	0.005406268181794778	0.0
22	0.0010297653679609102	0.0	0.0	0.007980681601697053	0.0
23	0.0010297653679609102	0.0	0.0	0.008495564285677508	0.0
24	0.0010297653679609102	0.0	0.0	0.0102976536796091	0.0
25	0.0010297653679609102	0.0	0.0	0.01235718441553092	0.0
26	0.0010297653679609102	0.0	0.0	0.015703921861403878	0.0
27	0.0010297653679609102	0.0	0.0	0.03372481580071981	0.0
28	0.0010297653679609102	0.0	0.0	0.10709559826793465	0.0
29	0.0012872067099511375	0.0	0.0	0.23813324134096045	0.0
30	0.0012872067099511375	0.0	0.0	0.398261756058882	0.0
31	0.0012872067099511375	0.0	0.0	0.819178350212904	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAA	30	3.5951304E-4	30.833332	19
TATATTC	30	3.5951304E-4	30.833332	3
TACTCTC	30	3.5951304E-4	30.833332	32
CTGTACT	25	0.0054919193	29.6	4
GTTAGAC	25	0.0054919193	29.6	2
TACTGGA	25	0.0054919193	29.6	2
GGTCTAT	25	0.0054919193	29.6	1
ATACGGC	55	6.2438266E-7	26.90909	29
TGCACGA	35	8.858782E-4	26.42857	10
GGTATCA	575	0.0	26.06087	1
TGTTTAC	60	1.3336012E-6	24.666666	14
ATCTTAC	60	1.3336012E-6	24.666666	1
CTTGTCG	40	0.0019288983	23.125	21
GTAAGTT	40	0.0019288983	23.125	29
TACGGCT	65	2.6752568E-6	22.76923	30
CTGTTTA	65	2.6752568E-6	22.76923	13
CTGCACG	50	2.6976413E-4	22.2	9
TTCTGCG	225	0.0	22.2	18
CCGTTTA	50	2.6976413E-4	22.2	27
AACGTCA	210	0.0	22.023811	28
>>END_MODULE