FastQCFastQC Report
Fri 10 Feb 2017
ERR1631925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481715
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16180.33588325046967604No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15020.31180262188223323No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13400.2781727785101149No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12450.2584515740635023No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11180.2320874375927675No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11040.22918115483221407No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9960.20676125925080183No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9830.2040625681160022No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9480.19679686121461862No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9020.18724764642994302No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8810.18288822228911286No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8620.17894398139979031No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7560.15693926906988573No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7270.150919111923025No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6810.14136989713834944No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6770.14053953063533417No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6500.1349345567399811No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6160.12787644146435131No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6090.12642330008407462No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6040.1253853419553055No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6000.12455497545229026No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5760.11957277643419864No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5480.11376021091309176No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5450.11313743603583032No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5400.11209947790706122No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5350.11106151977829215No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5340.11085392815253833No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5280.10960837839801543No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT5260.1091931951465078No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5240.10877801189500015No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5230.10857042026924633No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT5220.10836282864349252No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5030.10441858775416998No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5020.10421099612841617No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5010.10400340450266238No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4990.10358822125115473No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4980.10338062962540091No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG4970.1031730379996471No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG4920.10213507987087801No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4870.10109712174210891No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4860.1008895301163551No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4840.10047434686484746No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4840.10047434686484746No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4830.10026675523909366No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA4820.10005916361333984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6500.027.6076931
TAACCCT851.9699655E-923.94117735
ATACGGC851.9699655E-923.94117729
TTAACCC1155.456968E-1222.5217434
TATACAC1005.329639E-1022.237
GCTAAAC705.091775E-621.1428593
CTACCGT450.00382263120.5555558
TATACAA450.00382263120.5555555
ATTAGAG555.1371363E-420.1818183
TTGTACC555.1371363E-420.1818184
CTAAACC851.242237E-619.5882364
AACGACC851.242237E-619.58823637
TAAAGAC1457.2759576E-1219.1379337
GTATTAG1103.837704E-818.51
GGGCTAA701.2173322E-418.51
GACCGTT500.007029094318.57
GTTAGCA609.226198E-418.52
TTCAAAT609.226198E-418.528
TACAGGT701.2173322E-418.57
GTGGTTG500.007029094318.518
Produced by FastQC (version 0.11.5)