##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631925.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481715
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30607101709517	33.0	31.0	34.0	31.0	34.0
2	32.48494026550969	34.0	31.0	34.0	31.0	34.0
3	32.57487310961876	34.0	31.0	34.0	31.0	34.0
4	36.084427514194076	37.0	35.0	37.0	35.0	37.0
5	35.966621342495046	37.0	35.0	37.0	35.0	37.0
6	36.01636652377443	37.0	35.0	37.0	35.0	37.0
7	36.021817879866724	37.0	35.0	37.0	35.0	37.0
8	35.99807562562926	37.0	35.0	37.0	35.0	37.0
9	37.795929128218965	39.0	38.0	39.0	35.0	39.0
10	37.69159565303136	39.0	37.0	39.0	35.0	39.0
11	37.776940722211265	39.0	37.0	39.0	35.0	39.0
12	37.686673655584734	39.0	37.0	39.0	35.0	39.0
13	37.76039151780617	39.0	37.0	39.0	35.0	39.0
14	39.052726197025216	40.0	38.0	41.0	36.0	41.0
15	39.055941791308136	40.0	38.0	41.0	36.0	41.0
16	38.99116697632417	40.0	38.0	41.0	36.0	41.0
17	38.9612260361417	40.0	38.0	41.0	36.0	41.0
18	38.99134965695484	40.0	38.0	41.0	36.0	41.0
19	38.99822716751606	40.0	38.0	41.0	36.0	41.0
20	38.99096976427971	40.0	38.0	41.0	36.0	41.0
21	38.94522279771234	40.0	38.0	41.0	36.0	41.0
22	38.91789128426559	40.0	38.0	41.0	35.0	41.0
23	38.855651163032086	40.0	38.0	41.0	35.0	41.0
24	38.8592632573202	40.0	38.0	41.0	35.0	41.0
25	38.79891429579731	40.0	38.0	41.0	35.0	41.0
26	38.71814454604901	40.0	38.0	41.0	35.0	41.0
27	38.58735766999159	40.0	38.0	41.0	35.0	41.0
28	38.58572184798065	40.0	38.0	41.0	35.0	41.0
29	38.54658044694477	40.0	38.0	41.0	35.0	41.0
30	38.51770652771867	40.0	38.0	41.0	34.0	41.0
31	38.43723570991146	40.0	38.0	41.0	34.0	41.0
32	38.384193973615105	40.0	38.0	41.0	34.0	41.0
33	38.31700901985614	40.0	38.0	41.0	34.0	41.0
34	38.29129672109027	40.0	38.0	41.0	34.0	41.0
35	38.19152403392047	40.0	38.0	41.0	34.0	41.0
36	38.1424576772573	40.0	38.0	41.0	33.0	41.0
37	38.08520183095814	40.0	37.0	41.0	33.0	41.0
38	38.0383484010255	40.0	37.0	41.0	33.0	41.0
39	37.93340668237443	40.0	37.0	41.0	33.0	41.0
40	37.65113396925568	40.0	37.0	41.0	33.0	41.0
41	37.717787488452714	40.0	37.0	41.0	33.0	41.0
42	37.69388954049594	40.0	37.0	41.0	33.0	41.0
43	37.179093447370335	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	8.0
19	10.0
20	28.0
21	47.0
22	118.0
23	208.0
24	368.0
25	605.0
26	953.0
27	1450.0
28	2167.0
29	3231.0
30	4431.0
31	6098.0
32	8049.0
33	10867.0
34	15495.0
35	22836.0
36	35216.0
37	63165.0
38	141293.0
39	165069.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.830511817153294	16.664832940639172	12.755259852817538	27.749395389389992
2	21.27315944074816	20.051482723186947	31.701317168865405	26.974040667199482
3	21.511267035487787	19.94062879503441	29.48423860581464	29.06386556366316
4	15.549027952212407	16.22453110241533	34.87850700102758	33.34793394434469
5	16.547958855339775	32.9626438869456	33.90531745949368	16.58407979822094
6	33.02492137467175	34.514598881081135	15.857924291334088	16.60255545291303
7	29.693698556200243	27.85381397714416	20.207591625753818	22.24489584090178
8	26.960962394776995	30.215999086596845	20.760200533510478	22.062837985115678
9	28.399572361250947	12.459234194492593	17.72126672410035	41.419926720156106
10	19.333008106452986	23.20064768587235	29.638064000498222	27.82828020717644
11	39.23274135121389	19.30166177096416	18.9423206667843	22.523276211037647
12	22.227665735964212	24.04346968643285	26.858619723280363	26.87024485432258
13	35.293690252535214	17.73994997041819	20.524584038279894	26.441775738766697
14	24.044300052935867	20.10960837839802	23.14833459618239	32.69775697248373
15	29.782547772022877	24.04139377017531	19.88686256396417	26.28919589383764
16	25.08184299845344	24.24421078853679	22.844005272827296	27.829940940182475
17	26.863601922298454	23.91247937058219	21.608835099592085	27.615083607527275
18	26.312861339173576	21.454594521656997	23.567254496953595	28.665289642215836
19	28.008262146705004	22.43920160260735	23.52801967968612	26.024516571001527
20	30.242155631441825	20.6580654536396	22.52410657754066	26.57567233737791
21	27.318435174325067	22.126153430970593	21.9607029052448	28.594708489459535
22	28.348297229689756	22.8390230738092	21.344155776755965	27.468523919745074
23	28.067840943296346	22.1346646876265	22.324611025191242	27.472883343885908
24	27.779703766750046	21.699760231672254	22.664023333298736	27.85651266827896
25	28.169976023167226	22.10290316888617	23.016513913828714	26.710606894117888
26	27.943701151095564	22.900055011780825	22.213757097038705	26.942486740084902
27	27.061644333267598	21.77034138442855	24.0108778011895	27.157136481114353
28	26.24767756868688	23.122385642963163	21.99806939788049	28.631867390469466
29	27.16959197865958	23.624549785661646	22.119718090572228	27.086140145106548
30	26.914461870608143	22.027339817111777	23.76342858329095	27.29476972898913
31	28.096903770901882	22.575589300727607	22.578287991862407	26.7492189365081
32	25.88065557435413	22.21936207093406	22.845666005833323	29.054316348878483
33	26.04652128333143	22.092938770849983	24.31167806690678	27.548861878911808
34	26.526473122074258	22.245726207404793	23.56455580581879	27.663244864702158
35	25.845572589601733	24.122354504219302	23.922858951869884	26.109213954309084
36	26.52709589695152	22.777575952586073	24.307733826017458	26.38759432444495
37	27.222943026478312	22.63599846382197	23.871376228682937	26.26968228101678
38	26.3329977268717	22.34018039712278	24.546049012382838	26.78077286362268
39	25.710638032861755	21.55548405177335	25.214909230561638	27.51896868480326
40	25.67887651412142	22.32378065868823	25.966806099041968	26.030536728148384
41	23.954205287358707	21.55382331876732	26.8171013981296	27.674869995744373
42	22.65821076777763	22.016752644198334	27.71119853025129	27.61383805777275
43	22.139231703393087	22.667759982562302	27.21422417819665	27.978784135847963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	10.0
4	17.0
5	17.0
6	24.0
7	31.0
8	34.0
9	37.0
10	57.5
11	78.0
12	78.0
13	152.0
14	226.0
15	463.5
16	701.0
17	677.0
18	653.0
19	653.0
20	810.0
21	967.0
22	825.0
23	683.0
24	790.5
25	898.0
26	898.0
27	1041.5
28	1185.0
29	1593.5
30	2002.0
31	2625.5
32	3249.0
33	3249.0
34	4282.0
35	5315.0
36	6072.0
37	6829.0
38	8823.0
39	10817.0
40	10817.0
41	13070.0
42	15323.0
43	18382.5
44	21442.0
45	29408.5
46	37375.0
47	37375.0
48	41060.0
49	44745.0
50	50893.0
51	57041.0
52	57733.0
53	58425.0
54	58425.0
55	52819.0
56	47213.0
57	44476.5
58	41740.0
59	38564.5
60	35389.0
61	35389.0
62	32436.0
63	29483.0
64	24064.0
65	18645.0
66	16154.0
67	13663.0
68	13663.0
69	11688.5
70	9714.0
71	8302.0
72	6890.0
73	5573.0
74	4256.0
75	4256.0
76	3497.5
77	2739.0
78	2241.0
79	1743.0
80	1376.0
81	1009.0
82	1009.0
83	798.5
84	588.0
85	461.5
86	335.0
87	244.5
88	154.0
89	154.0
90	116.5
91	79.0
92	49.5
93	20.0
94	16.0
95	12.0
96	12.0
97	6.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	481715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.168928076880896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.9061670180385	39.661956464163374
2	6.559715228533171	6.057100411819365
3	2.1563027122156626	2.986625544968044
4	1.1227933520610773	2.0735266206603153
5	0.7168787296817541	1.6548761255261322
6	0.49516814913120905	1.3716829597920612
7	0.3588264508669199	1.1596642821510084
8	0.2837587994509741	1.0480671682427274
9	0.2241671694997285	0.9314602133247841
>10	1.7895320730028907	16.829831065433122
>50	0.21960749882474606	7.081023883386421
>100	0.1512348548606801	13.53614479360844
>500	0.013131170032813545	3.9508818602997056
>1k	0.0027167937998924573	1.6571586066244652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1618	0.33588325046967604	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1502	0.31180262188223323	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1340	0.2781727785101149	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1245	0.2584515740635023	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1118	0.2320874375927675	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1104	0.22918115483221407	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	996	0.20676125925080183	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	983	0.2040625681160022	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	948	0.19679686121461862	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	902	0.18724764642994302	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	881	0.18288822228911286	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	862	0.17894398139979031	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	756	0.15693926906988573	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	727	0.150919111923025	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	681	0.14136989713834944	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	677	0.14053953063533417	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	650	0.1349345567399811	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	616	0.12787644146435131	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	609	0.12642330008407462	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	604	0.1253853419553055	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	600	0.12455497545229026	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	576	0.11957277643419864	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	548	0.11376021091309176	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	545	0.11313743603583032	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	540	0.11209947790706122	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	535	0.11106151977829215	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	534	0.11085392815253833	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	528	0.10960837839801543	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	526	0.1091931951465078	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	524	0.10877801189500015	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	523	0.10857042026924633	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	522	0.10836282864349252	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	503	0.10441858775416998	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	502	0.10421099612841617	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	501	0.10400340450266238	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	499	0.10358822125115473	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	498	0.10338062962540091	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	497	0.1031730379996471	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	492	0.10213507987087801	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	487	0.10109712174210891	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	486	0.1008895301163551	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	484	0.10047434686484746	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	484	0.10047434686484746	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	483	0.10026675523909366	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	482	0.10005916361333984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.075916257538171E-4	0.0
10	2.075916257538171E-4	0.0	0.0	2.075916257538171E-4	0.0
11	2.075916257538171E-4	0.0	0.0	2.075916257538171E-4	0.0
12	2.075916257538171E-4	0.0	0.0	2.075916257538171E-4	0.0
13	2.075916257538171E-4	0.0	0.0	2.075916257538171E-4	0.0
14	2.075916257538171E-4	0.0	0.0	2.075916257538171E-4	0.0
15	2.075916257538171E-4	0.0	0.0	2.075916257538171E-4	0.0
16	2.075916257538171E-4	0.0	0.0	6.227748772614513E-4	0.0
17	2.075916257538171E-4	0.0	0.0	8.303665030152684E-4	0.0
18	6.227748772614513E-4	0.0	0.0	8.303665030152684E-4	0.0
19	6.227748772614513E-4	0.0	0.0	0.0012455497545229026	0.0
20	6.227748772614513E-4	0.0	0.0	0.002491099509045805	0.0
21	6.227748772614513E-4	0.0	0.0	0.003944240889322524	0.0
22	6.227748772614513E-4	0.0	0.0	0.005189790643845427	0.0
23	6.227748772614513E-4	0.0	0.0	0.006227748772614513	0.0
24	8.303665030152684E-4	0.0	0.0	0.007265706901383598	0.0
25	8.303665030152684E-4	0.0	0.0	0.008303665030152684	0.0
26	8.303665030152684E-4	0.0	0.0	0.010794764539198488	0.0
27	0.0010379581287690855	0.0	0.0	0.02574136159347332	0.0
28	0.0010379581287690855	0.0	0.0	0.0776392680319276	0.0
29	0.0010379581287690855	0.0	0.0	0.17188586612416054	0.0
30	0.0010379581287690855	0.0	0.0	0.2914586425583592	0.0
31	0.0010379581287690855	0.0	0.0	0.5735756619577966	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	650	0.0	27.607693	1
TAACCCT	85	1.9699655E-9	23.941177	35
ATACGGC	85	1.9699655E-9	23.941177	29
TTAACCC	115	5.456968E-12	22.52174	34
TATACAC	100	5.329639E-10	22.2	37
GCTAAAC	70	5.091775E-6	21.142859	3
CTACCGT	45	0.003822631	20.555555	8
TATACAA	45	0.003822631	20.555555	5
ATTAGAG	55	5.1371363E-4	20.181818	3
TTGTACC	55	5.1371363E-4	20.181818	4
CTAAACC	85	1.242237E-6	19.588236	4
AACGACC	85	1.242237E-6	19.588236	37
TAAAGAC	145	7.2759576E-12	19.13793	37
GTATTAG	110	3.837704E-8	18.5	1
GGGCTAA	70	1.2173322E-4	18.5	1
GACCGTT	50	0.0070290943	18.5	7
GTTAGCA	60	9.226198E-4	18.5	2
TTCAAAT	60	9.226198E-4	18.5	28
TACAGGT	70	1.2173322E-4	18.5	7
GTGGTTG	50	0.0070290943	18.5	18
>>END_MODULE