FastQCFastQC Report
Fri 10 Feb 2017
ERR1631924.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631924.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350966
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17140.4883663944655608No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15300.43593966367112486No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14860.4234028367420206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14260.4063071636568784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.37297060114085123No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12660.36071870209649937No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11990.3416285338180906No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11630.33137112996700535No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11480.3270972116957198No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10810.3080070434173111No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10480.2986044232204829No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10240.291766153986426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10220.2911962982169213No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9620.27410062513177913No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9170.2612788703179225No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9070.25842959147039885No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8830.25159132223634195No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8790.2504516106973325No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7740.22053418279833373No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7660.2182547597203148No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7660.2182547597203148No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC7640.21768490395081005No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7580.21597533664229585No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA7500.21369591356427686No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7300.2079973558692295No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7080.20172894240467737No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6980.1988796635571537No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6940.1977399520181442No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6920.19717009624863946No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6770.19289617797735392No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6600.18805240393656367No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6470.18434834143478285No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6370.18149906258725917No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC6310.17978949527874497No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6290.17921963950924022No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6270.17864978373973547No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6250.17807992797023073No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6230.17751007220072598No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6210.17694021643122124No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG6090.17352108181419282No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6000.1709567308514215No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5770.164403389502117No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5750.16383353373261228No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5700.16240889430885044No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC5680.1618390385393457No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT5640.16069932700033623No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5520.1572801923833078No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5460.15557062507479358No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC5450.1552856971900412No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5230.14901728372548909No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG5060.1441735096846988No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4980.14189408660667985No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4940.14075437506767038No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4940.14075437506767038No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4910.13989959141341327No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4880.13904480775915615No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA4880.13904480775915615No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4660.13277639429460403No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4610.1313517548708422No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4600.13106682698608982No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG4560.12992711544708033No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4530.12907233179282324No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG4480.1276476923690614No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4450.12679290871480428No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG4440.1265079808300519No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4430.12622305294529954No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT4410.12565319717579482No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4410.12565319717579482No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4340.12365870198252822No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA4280.12194913467401401No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4210.11995463948074742No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC4150.1182450721722332No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4130.11767521640272847No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT4110.11710536063322373No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4100.11682043274847136No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA4080.11625057697896661No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4070.11596564909421427No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3940.11226158659243346No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3860.10998216351441449No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG3820.10884245197540503No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA3810.10855752409065265No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3790.10798766832114792No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT3760.1071328846668908No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3760.1071328846668908No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA3740.10656302889738609No Hit
GGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTAC3650.10399867793461474No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3640.10371375004986237No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC3630.10342882216511003No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3610.10285896639560528No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3560.10143432697184343No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3540.10086447120233868No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG3530.10057954331758631No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC3520.10029461543283394No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3520.10029461543283394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGTC308.2955285E-637.034
CCTAGTT352.3836174E-531.7142852
GCCTAGT352.3836174E-531.7142851
ATACGGC705.456968E-1231.71428529
ACTAACT303.5944293E-430.83333435
AACCGTG250.005491207429.5999987
GGTATCA9750.029.4102551
TAGTTCC405.9285554E-527.7500024
CTAGTTC405.9285554E-527.7500023
CTAACTG358.8570727E-426.4285736
TAGGACC706.559276E-926.428574
TACGGCT951.0913936E-1125.3157930
TTAGGAC751.3667886E-824.6666663
ATTGTTA451.320655E-424.66666614
TAAGTCT451.320655E-424.66666635
TACGAGG551.897781E-523.5454542
TTACGAG551.897781E-523.5454541
GACCGTT802.7121132E-823.1250027
TGGTCAT400.001928529223.12500232
AGGACCG903.805326E-922.611115
Produced by FastQC (version 0.11.5)