FastQCFastQC Report
Fri 10 Feb 2017
ERR1631923.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631923.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12847
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC520.4047637580758154No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA410.31914065540593134No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA380.2957889001323266No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC310.24130147116058223No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT240.18681404218883785No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA230.17903012376430297No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT220.17124620533976806No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA210.16346228691523312No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA200.15567836849069822No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG200.15567836849069822No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA200.15567836849069822No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG200.15567836849069822No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA200.15567836849069822No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT190.1478944500661633No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT190.1478944500661633No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG180.1401105316416284No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT180.1401105316416284No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG170.1323266132170935No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA170.1323266132170935No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT160.12454269479255857No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC160.12454269479255857No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC150.11675877636802365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150.11675877636802365No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT150.11675877636802365No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT150.11675877636802365No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT140.10897485794348874No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC140.10897485794348874No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT130.10119093951895385No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC130.10119093951895385No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA130.10119093951895385No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC130.10119093951895385No Hit
GCCCCACACCCGCCGCCTCCTGCACCGAGAGAGATGGAATAAA130.10119093951895385No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG130.10119093951895385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG250.005298204329.67
ACGCAGA250.005298204329.68
GTATCAA250.005298204329.62
TCAACGC250.005298204329.65
CAGAGTA250.005298204329.611
AGAGTAC250.005298204329.612
GCAGAGT250.005298204329.610
GGTATCA250.005298204329.61
CTTTAGT400.001829390223.1252
TTTAGTG400.001829390223.1253
TCTTTAG400.001829390223.1251
Produced by FastQC (version 0.11.5)