##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631923.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12847
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.432318829298666	33.0	31.0	34.0	31.0	34.0
2	32.609247295088345	34.0	31.0	34.0	31.0	34.0
3	32.711605822370984	34.0	31.0	34.0	31.0	34.0
4	36.14867284190861	37.0	35.0	37.0	35.0	37.0
5	36.07612672219195	37.0	35.0	37.0	35.0	37.0
6	36.099945512571026	37.0	35.0	37.0	35.0	37.0
7	36.09792169378065	37.0	35.0	37.0	35.0	37.0
8	36.095664357437535	37.0	35.0	37.0	35.0	37.0
9	37.92753171946758	39.0	38.0	39.0	35.0	39.0
10	37.85848836304196	39.0	38.0	39.0	35.0	39.0
11	37.934148050128435	39.0	38.0	39.0	35.0	39.0
12	37.8477465556161	39.0	38.0	39.0	35.0	39.0
13	37.92075971043823	39.0	38.0	39.0	35.0	39.0
14	39.27111387872655	40.0	39.0	41.0	36.0	41.0
15	39.257492021483614	40.0	39.0	41.0	36.0	41.0
16	39.213824239121976	40.0	39.0	41.0	36.0	41.0
17	39.19358605121818	40.0	39.0	41.0	36.0	41.0
18	39.12999143768973	40.0	39.0	41.0	36.0	41.0
19	39.18082042500195	40.0	39.0	41.0	36.0	41.0
20	39.13964349653616	40.0	39.0	41.0	36.0	41.0
21	39.13209309566436	40.0	39.0	41.0	36.0	41.0
22	39.11146571183934	40.0	39.0	41.0	36.0	41.0
23	39.01751381645521	40.0	39.0	41.0	35.0	41.0
24	38.99665291507745	40.0	39.0	41.0	35.0	41.0
25	38.93835136607768	40.0	38.0	41.0	35.0	41.0
26	38.885265042422354	40.0	38.0	41.0	35.0	41.0
27	38.7263952673776	40.0	38.0	41.0	35.0	41.0
28	38.697983965128046	40.0	38.0	41.0	35.0	41.0
29	38.68054798785709	40.0	38.0	41.0	35.0	41.0
30	38.641939752471394	40.0	38.0	41.0	35.0	41.0
31	38.556550167354246	40.0	38.0	41.0	34.0	41.0
32	38.506733089437226	40.0	38.0	41.0	34.0	41.0
33	38.47139409978983	40.0	38.0	41.0	34.0	41.0
34	38.421577021872814	40.0	38.0	41.0	34.0	41.0
35	38.31415894761423	40.0	38.0	41.0	34.0	41.0
36	38.27064684362108	40.0	38.0	41.0	34.0	41.0
37	38.24581614384681	40.0	37.0	41.0	33.0	41.0
38	38.20619599906593	40.0	37.0	41.0	34.0	41.0
39	38.06382813108119	40.0	37.0	41.0	33.0	41.0
40	37.80804857165097	40.0	37.0	41.0	33.0	41.0
41	37.88510936405387	40.0	37.0	41.0	33.0	41.0
42	37.8158324900755	40.0	37.0	41.0	33.0	41.0
43	37.35167743442049	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	3.0
23	6.0
24	6.0
25	15.0
26	23.0
27	38.0
28	66.0
29	80.0
30	118.0
31	157.0
32	197.0
33	267.0
34	397.0
35	638.0
36	863.0
37	1356.0
38	3203.0
39	5410.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55880750369736	15.941464933447497	11.543551023585273	28.95617653926987
2	21.35128823849926	18.38561531875146	31.556005293064526	28.70709114968475
3	21.133338522612284	19.195142834903088	28.16221685996731	31.50930178251732
4	16.315093017825173	14.532575698606678	32.536779014555925	36.61555226901222
5	17.646143068420642	32.84035183311279	32.41223631976337	17.1012687787032
6	36.03954230559664	33.322954775433956	14.275706390597026	16.361796528372384
7	31.773955008951503	26.838950727796373	19.615474429827977	21.771619833424147
8	28.551412781194053	30.077060792402893	19.078384058535068	22.293142367867986
9	28.683739394411145	11.722581147349576	16.525258815287618	43.06842064295166
10	19.942399003658444	22.495524246905894	28.714875068109286	28.84720168132638
11	40.68654160504398	17.97306764225111	18.49459017669495	22.845800576009964
12	24.51934303728497	22.65898653382113	25.28216704288939	27.539503386004515
13	34.74741184712384	16.75877636802366	20.479489374951353	28.014322409901144
14	23.826574297501363	19.22627850860123	22.76017747334008	34.18696972055733
15	30.450688876780575	22.55779559430217	19.537635245582628	27.45388028333463
16	26.63656884875846	22.137463999377285	20.853117459329027	30.37284969253522
17	26.737759788277423	24.083443605511015	20.362730598583326	28.816066007628237
18	26.909005993617185	19.771152798318674	23.639760255312524	29.680080952751613
19	28.62146804701487	21.250097298980304	22.0284891414338	28.09994551257103
20	29.742352300147896	20.946524480423445	21.63150930178252	27.679613917646144
21	29.711216626449755	20.277107495913445	21.265665135829376	28.746010741807424
22	30.723126021639292	22.246438857320776	19.942399003658444	27.088036117381492
23	29.50883474741185	22.23865493889624	19.631042266677046	28.62146804701487
24	28.870553436599984	20.77527827508368	21.452479178018212	28.901689110298122
25	29.314236786798475	20.938740561998912	22.0284891414338	27.718533509768818
26	29.594457850081728	21.265665135829376	21.958433875612986	27.18144313847591
27	29.19747801043045	20.930956643574376	22.370981552113335	27.500583793881837
28	26.893438156768116	21.273449054253913	21.436911341169147	30.396201447808828
29	29.142990581458704	22.441036817934148	21.483614851716354	26.93235774889079
30	28.053242002023822	20.635167743442047	23.78765470537869	27.523935549155443
31	28.40351833112789	21.32015256480112	21.865026854518565	28.411302249552424
32	25.577955943021717	21.374639993772863	22.66677045224566	30.380633610959755
33	27.1347396279287	21.849459017669496	23.437378376274616	27.578422978127186
34	27.555071222853584	21.468047014867285	22.690122207519263	28.286759554759865
35	27.881995796684052	22.402117225811473	23.811006460652294	25.904880516852185
36	28.107729430995565	21.538102280688097	22.721257881217404	27.632910407098933
37	26.722191951428346	21.74826807815054	23.66311201058613	27.866427959834983
38	27.126955709504163	20.689655172413794	24.145714952907294	28.037674165174746
39	25.60909161671986	20.98544407254612	25.3288705534366	28.076593757297424
40	25.764769985210556	21.17225811473496	26.59764925663579	26.4653226434187
41	24.091227523935547	20.713006927687395	26.768895461975557	28.426870086401497
42	22.47217249163229	22.075192651981006	27.648478243948006	27.804156612438703
43	21.693780649178795	20.028022106328326	28.115513349420095	30.162683895072778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	1.5
11	2.0
12	2.0
13	8.0
14	14.0
15	18.0
16	22.0
17	21.5
18	21.0
19	21.0
20	24.0
21	27.0
22	22.0
23	17.0
24	18.5
25	20.0
26	20.0
27	16.5
28	13.0
29	24.5
30	36.0
31	48.0
32	60.0
33	60.0
34	91.5
35	123.0
36	133.5
37	144.0
38	194.5
39	245.0
40	245.0
41	291.5
42	338.0
43	436.0
44	534.0
45	670.5
46	807.0
47	807.0
48	865.0
49	923.0
50	1110.0
51	1297.0
52	1341.0
53	1385.0
54	1385.0
55	1344.0
56	1303.0
57	1246.0
58	1189.0
59	1103.0
60	1017.0
61	1017.0
62	1001.5
63	986.0
64	787.0
65	588.0
66	518.0
67	448.0
68	448.0
69	386.0
70	324.0
71	300.5
72	277.0
73	294.0
74	311.0
75	311.0
76	267.5
77	224.0
78	154.0
79	84.0
80	58.0
81	32.0
82	32.0
83	24.0
84	16.0
85	12.0
86	8.0
87	7.0
88	6.0
89	6.0
90	5.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12847.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.76406943255235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25374411638853	62.76173425702498
2	6.9426615318784775	10.103526115046314
3	2.706461275139067	5.907994084221997
4	1.4013692768506634	4.078773254456293
5	0.6418485237483954	2.335175527360473
6	0.5455712451861361	2.381879037907683
7	0.363714163457424	1.8525725850393089
8	0.23534445870774498	1.3699696427181445
9	0.2246469833119384	1.4711605822370981
>10	0.6739409499358151	7.332451155911886
>50	0.01069747539580659	0.4047637580758154
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	52	0.4047637580758154	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	41	0.31914065540593134	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	38	0.2957889001323266	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	31	0.24130147116058223	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	24	0.18681404218883785	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	23	0.17903012376430297	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	22	0.17124620533976806	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	21	0.16346228691523312	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	20	0.15567836849069822	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	20	0.15567836849069822	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	20	0.15567836849069822	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	20	0.15567836849069822	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	20	0.15567836849069822	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	19	0.1478944500661633	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	19	0.1478944500661633	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	18	0.1401105316416284	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	18	0.1401105316416284	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	17	0.1323266132170935	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	17	0.1323266132170935	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	16	0.12454269479255857	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	16	0.12454269479255857	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	15	0.11675877636802365	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.11675877636802365	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	15	0.11675877636802365	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	15	0.11675877636802365	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	14	0.10897485794348874	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	14	0.10897485794348874	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	13	0.10119093951895385	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	13	0.10119093951895385	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	13	0.10119093951895385	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	13	0.10119093951895385	No Hit
GCCCCACACCCGCCGCCTCCTGCACCGAGAGAGATGGAATAAA	13	0.10119093951895385	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	13	0.10119093951895385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.007783918424534911	0.0	0.0	0.0	0.0
19	0.007783918424534911	0.0	0.0	0.0	0.0
20	0.007783918424534911	0.0	0.0	0.0	0.0
21	0.007783918424534911	0.0	0.0	0.0	0.0
22	0.007783918424534911	0.0	0.0	0.0	0.0
23	0.007783918424534911	0.0	0.0	0.0	0.0
24	0.007783918424534911	0.0	0.0	0.0	0.0
25	0.007783918424534911	0.0	0.0	0.007783918424534911	0.0
26	0.007783918424534911	0.0	0.0	0.015567836849069821	0.0
27	0.007783918424534911	0.0	0.0	0.031135673698139642	0.0
28	0.007783918424534911	0.0	0.0	0.15567836849069822	0.0
29	0.007783918424534911	0.0	0.0	0.33470849225500116	0.0
30	0.007783918424534911	0.0	0.0	0.5059546975947692	0.0
31	0.007783918424534911	0.0	0.0	0.9652058846423289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	25	0.0052982043	29.6	7
ACGCAGA	25	0.0052982043	29.6	8
GTATCAA	25	0.0052982043	29.6	2
TCAACGC	25	0.0052982043	29.6	5
CAGAGTA	25	0.0052982043	29.6	11
AGAGTAC	25	0.0052982043	29.6	12
GCAGAGT	25	0.0052982043	29.6	10
GGTATCA	25	0.0052982043	29.6	1
CTTTAGT	40	0.0018293902	23.125	2
TTTAGTG	40	0.0018293902	23.125	3
TCTTTAG	40	0.0018293902	23.125	1
>>END_MODULE