FastQCFastQC Report
Fri 10 Feb 2017
ERR1631922.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631922.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011234
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12490.12351246101298018No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11360.11233799496456803No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11170.1104591024431536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.031.21
ACACGAA400.001931027723.12517
ATACGGC1107.4578566E-1121.86363629
GCGGTAA6150.021.35772323
CGCGGTA6300.020.84920722
GGACGTA450.003825494320.55555727
GGTAATA6650.019.75187925
CGGTAAT6700.019.32835824
AGCCGCG6800.019.04411719
GTAACGT3150.018.79365226
AACTCCG7250.018.6275865
TAGACCC500.00703431218.4999984
CTTATAC11200.018.16964337
GCTAACT7300.017.993152
TAATACG6600.017.93939427
CTAACTC7550.017.8874193
TAACTCC7600.017.7697374
GGTAACG3450.017.69565225
CGTCAAT3150.017.6190530
TAAATCC1054.7958565E-717.6190474
Produced by FastQC (version 0.11.5)