##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631922.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1011234
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.448094110759726	33.0	31.0	34.0	31.0	34.0
2	32.62794170290952	34.0	31.0	34.0	31.0	34.0
3	32.72138001689026	34.0	31.0	34.0	31.0	34.0
4	36.21036377336996	37.0	37.0	37.0	35.0	37.0
5	36.102708176346916	37.0	35.0	37.0	35.0	37.0
6	36.14218469711264	37.0	36.0	37.0	35.0	37.0
7	36.12964457286839	37.0	36.0	37.0	35.0	37.0
8	36.11134910416382	37.0	35.0	37.0	35.0	37.0
9	37.92890765144368	39.0	38.0	39.0	35.0	39.0
10	37.843854142562456	39.0	38.0	39.0	35.0	39.0
11	37.92180049325873	39.0	38.0	39.0	35.0	39.0
12	37.850724955846026	39.0	38.0	39.0	35.0	39.0
13	37.9056884954422	39.0	38.0	39.0	35.0	39.0
14	39.264406655630644	40.0	39.0	41.0	36.0	41.0
15	39.25608612843318	40.0	39.0	41.0	36.0	41.0
16	39.212155643500914	40.0	39.0	41.0	36.0	41.0
17	39.17772444360059	40.0	39.0	41.0	36.0	41.0
18	39.19272295037548	40.0	39.0	41.0	36.0	41.0
19	39.22052363745681	40.0	39.0	41.0	36.0	41.0
20	39.20354537129883	40.0	39.0	41.0	36.0	41.0
21	39.17235081098934	40.0	39.0	41.0	36.0	41.0
22	39.148337575674866	40.0	39.0	41.0	36.0	41.0
23	39.0789085414454	40.0	39.0	41.0	36.0	41.0
24	39.09847967928294	40.0	39.0	41.0	36.0	41.0
25	39.04286050508586	40.0	39.0	41.0	36.0	41.0
26	38.97790125727576	40.0	38.0	41.0	35.0	41.0
27	38.873376488527875	40.0	38.0	41.0	35.0	41.0
28	38.86141783207447	40.0	38.0	41.0	35.0	41.0
29	38.833179066368416	40.0	38.0	41.0	35.0	41.0
30	38.791807830828475	40.0	38.0	41.0	35.0	41.0
31	38.718262044195505	40.0	38.0	41.0	35.0	41.0
32	38.66991121738391	40.0	38.0	41.0	35.0	41.0
33	38.61257829542915	40.0	38.0	41.0	35.0	41.0
34	38.579533520431475	40.0	38.0	41.0	35.0	41.0
35	38.51535055189996	40.0	38.0	41.0	35.0	41.0
36	38.470289764782436	40.0	38.0	41.0	34.0	41.0
37	38.43755451260539	40.0	38.0	41.0	34.0	41.0
38	38.40691570892593	40.0	38.0	41.0	34.0	41.0
39	38.35758884689399	40.0	38.0	41.0	34.0	41.0
40	38.06704778518127	40.0	37.0	41.0	34.0	41.0
41	38.14579612631695	40.0	37.0	41.0	34.0	41.0
42	38.13039514098616	40.0	37.0	41.0	34.0	41.0
43	37.65097000298645	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	1.0
17	5.0
18	14.0
19	27.0
20	51.0
21	104.0
22	169.0
23	355.0
24	581.0
25	998.0
26	1560.0
27	2429.0
28	3569.0
29	5434.0
30	7599.0
31	10641.0
32	14424.0
33	19654.0
34	28558.0
35	42258.0
36	65601.0
37	116594.0
38	264455.0
39	426152.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.66114470043531	17.155969834875016	12.868139322847135	27.314746141842537
2	20.19661126900401	19.7624882074772	33.63771392180247	26.403186601716317
3	20.490015169584883	20.286006997391308	29.37411123439283	29.84986659863098
4	15.518070001602002	15.634956894249996	33.621595001750336	35.22537810239766
5	16.582413170443242	33.77319196150446	33.30505105643204	16.339343811620257
6	35.07190225012213	33.911241117288384	15.213194967732493	15.803661664856996
7	30.98056434020217	28.343983687257353	19.730349256453007	20.945102716087476
8	27.50906318418882	32.274626842056335	19.67497137161132	20.54133860214352
9	27.92251842798007	13.4873827422733	17.564381735582465	41.02571709416416
10	18.577599250025216	24.51124072173206	30.622684759412756	26.288475268829963
11	38.6798703366382	20.072307695350432	19.59131120986834	21.65651075814302
12	23.252976066864843	23.724676978819936	27.249380459913336	25.77296649440189
13	33.966421223969924	17.8966490446326	21.7312709026793	26.40565882871818
14	24.03429868853302	19.408465300810693	23.67711133130413	32.88012467935216
15	28.281386899570226	24.903731480547528	20.524626347610937	26.290255272271306
16	25.907060086982835	24.22100127171357	22.291081984980725	27.580856656322865
17	26.407834388479817	24.00789530415314	22.718777256302698	26.865493051064345
18	26.500987901909944	22.33746096353564	23.726456982261276	27.43509415229314
19	28.089937640546104	22.706119454053166	23.462126471222287	25.74181643417844
20	28.78057897578602	21.983042500548837	23.109488011676824	26.12689051198832
21	27.330469505574378	22.917742085412478	22.294345324623183	27.457443084389965
22	27.681327961678505	23.425043066194373	21.876736739468807	27.016892232658318
23	27.240084886386335	22.971834412213195	22.786615165233766	27.0014655361667
24	27.278750516695442	22.48500347100671	22.90557872856332	27.330667283734527
25	27.575417756918775	22.5001335002581	23.215694883676775	26.70875385914635
26	27.249874905313703	22.838531932272847	23.066570150924516	26.845023011488934
27	26.92977095311273	22.525350215677083	23.60769119709187	26.937187634118313
28	26.366795420248923	23.336537339527748	22.846443058678805	27.45022418154453
29	26.651398192703173	23.759980380406514	22.87749422982218	26.711127197068134
30	26.425139977492844	23.462522027542587	23.484277625158963	26.628060369805606
31	27.133680236226233	22.988052221345406	23.122838037486872	26.755429504941485
32	25.112486328584684	23.12501359724851	23.65238906128552	28.11011101288129
33	25.541170490707394	22.64609378244798	24.77982346321425	27.032912263630376
34	25.925156788636457	22.262898597159513	24.57215639505792	27.23978821914611
35	25.670715185604916	23.173568135565063	24.858934727273805	26.29678195155622
36	25.653409596591885	22.50369350714078	25.323416736383468	26.519480159883862
37	25.774449830603007	21.979680271826304	24.97651384348232	27.269356054088373
38	24.761034538000107	21.60904399970729	26.336139805425844	27.293781656866763
39	24.397814946886676	20.67503663840417	27.396230743823885	27.530917670885273
40	23.975855242209025	21.251955531558473	28.255477960590724	26.516711265641778
41	23.066570150924516	20.578619785331583	29.242588757893824	27.11222130585008
42	22.185369558381147	20.78460573912665	29.757603086921524	27.27242161557068
43	21.187282073189788	20.862134777905013	30.06109367366999	27.88948947523521
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	11.0
2	14.0
3	23.0
4	32.0
5	32.0
6	61.0
7	90.0
8	92.5
9	95.0
10	138.0
11	181.0
12	181.0
13	360.0
14	539.0
15	1167.5
16	1796.0
17	1782.0
18	1768.0
19	1768.0
20	2004.5
21	2241.0
22	2307.0
23	2373.0
24	2933.0
25	3493.0
26	3493.0
27	4230.5
28	4968.0
29	6959.0
30	8950.0
31	10709.5
32	12469.0
33	12469.0
34	15320.5
35	18172.0
36	20743.0
37	23314.0
38	27624.0
39	31934.0
40	31934.0
41	37046.0
42	42158.0
43	46752.5
44	51347.0
45	61685.5
46	72024.0
47	72024.0
48	78825.0
49	85626.0
50	92373.0
51	99120.0
52	102377.0
53	105634.0
54	105634.0
55	99143.5
56	92653.0
57	88222.5
58	83792.0
59	77911.0
60	72030.0
61	72030.0
62	65961.0
63	59892.0
64	51251.0
65	42610.0
66	36885.5
67	31161.0
68	31161.0
69	26600.0
70	22039.0
71	18777.0
72	15515.0
73	12504.0
74	9493.0
75	9493.0
76	7699.5
77	5906.0
78	4728.0
79	3550.0
80	2770.5
81	1991.0
82	1991.0
83	1562.5
84	1134.0
85	872.5
86	611.0
87	471.5
88	332.0
89	332.0
90	235.5
91	139.0
92	82.0
93	25.0
94	18.5
95	12.0
96	12.0
97	7.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1011234.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.04789703555073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02081355513653	53.73485562899384
2	6.423544227096806	7.842877331582243
3	1.915032480241748	3.5072611702060073
4	0.9236126115228288	2.255384304359271
5	0.54444852646933	1.6618718792528493
6	0.3596708345635003	1.31742888450739
7	0.25736100024218816	1.0997943490626017
8	0.18937759470203364	0.9248883121768007
9	0.1554163555350053	0.8539057503307393
>10	1.0267327247993623	12.28917910889526
>50	0.11459516740517504	4.848469572555224
>100	0.06305674469108104	7.095348551763567
>500	0.005850625471804217	2.221289691966996
>1k	4.8755212265035145E-4	0.3474454643471535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1249	0.12351246101298018	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1136	0.11233799496456803	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1117	0.1104591024431536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	9.888908007444369E-5	0.0	0.0	0.0	0.0
9	9.888908007444369E-5	0.0	0.0	0.0	0.0
10	9.888908007444369E-5	0.0	0.0	0.0	0.0
11	9.888908007444369E-5	0.0	0.0	0.0	0.0
12	1.9777816014888739E-4	0.0	0.0	0.0	0.0
13	1.9777816014888739E-4	0.0	0.0	9.888908007444369E-5	0.0
14	1.9777816014888739E-4	0.0	0.0	9.888908007444369E-5	0.0
15	1.9777816014888739E-4	0.0	0.0	2.966672402233311E-4	0.0
16	2.966672402233311E-4	0.0	0.0	6.922235605211059E-4	0.0
17	2.966672402233311E-4	0.0	0.0	0.0011866689608933244	0.0
18	3.9555632029777477E-4	0.0	0.0	0.001582225281191099	0.0
19	3.9555632029777477E-4	0.0	0.0	0.0018788925214144304	0.0
20	3.9555632029777477E-4	0.0	0.0	0.002373337921786649	0.0
21	3.9555632029777477E-4	0.0	0.0	0.003955563202977748	0.0
22	3.9555632029777477E-4	0.0	0.0	0.006032233884541066	0.0
23	3.9555632029777477E-4	0.0	0.0	0.007021124685285503	0.0
24	3.9555632029777477E-4	0.0	0.0	0.008998906286774376	0.0
25	3.9555632029777477E-4	0.0	0.0	0.009691129847295482	0.0
26	4.944454003722185E-4	0.0	0.0	0.011372244208561026	0.0
27	4.944454003722185E-4	0.0	0.0	0.028974500461812006	0.0
28	4.944454003722185E-4	0.0	0.0	0.12845691501670237	0.0
29	4.944454003722185E-4	0.0	0.0	0.2789660948900057	0.0
30	4.944454003722185E-4	0.0	0.0	0.4745686952772553	0.0
31	4.944454003722185E-4	0.0	0.0	1.084912097496722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	925	0.0	31.2	1
ACACGAA	40	0.0019310277	23.125	17
ATACGGC	110	7.4578566E-11	21.863636	29
GCGGTAA	615	0.0	21.357723	23
CGCGGTA	630	0.0	20.849207	22
GGACGTA	45	0.0038254943	20.555557	27
GGTAATA	665	0.0	19.751879	25
CGGTAAT	670	0.0	19.328358	24
AGCCGCG	680	0.0	19.044117	19
GTAACGT	315	0.0	18.793652	26
AACTCCG	725	0.0	18.627586	5
TAGACCC	50	0.007034312	18.499998	4
CTTATAC	1120	0.0	18.169643	37
GCTAACT	730	0.0	17.99315	2
TAATACG	660	0.0	17.939394	27
CTAACTC	755	0.0	17.887419	3
TAACTCC	760	0.0	17.769737	4
GGTAACG	345	0.0	17.695652	25
CGTCAAT	315	0.0	17.61905	30
TAAATCC	105	4.7958565E-7	17.619047	4
>>END_MODULE