FastQCFastQC Report
Fri 10 Feb 2017
ERR1631920.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631920.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318378
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7970.25033136711707465No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7220.2267744630596335No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6520.20478801927268844No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6470.20321755900219235No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6310.1981920861366049No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5390.1692956171594771No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5170.16238559196929436No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4790.15045009391352418No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4670.1466809892643336No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT4570.14354006872334144No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4560.14322597666924222No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4360.1369441355872579No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4150.1303482024511744No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4090.1284636501265791No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3930.12343817726099164No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3890.1221818090445948No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3790.11904088850360264No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3450.10836175866422931No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG3270.10270810169044345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATA308.293107E-637.04
CTATGCT303.5936866E-430.8333324
TAGCGAA250.0054904529.59999810
CCGTCTC250.0054904529.5999988
TAAGGTA250.0054904529.5999985
CAACTAT250.0054904529.59999814
TAGATAC405.9268517E-527.755
ATACGGC405.9268517E-527.7529
GGTATCA3450.027.3478241
GTAAGAT358.8552554E-426.4285723
TAAGATT358.8552554E-426.4285724
CCGAAAT659.347241E-825.61538524
ATGCAGG701.9138497E-723.78571536
GCCGACC400.001928137823.1258
CCTGGTA400.001928137823.12512
AAGATTT400.001928137823.1255
GCGGTAA2200.022.70454623
AGCCGCG2300.022.5217419
GAAATTT753.7232167E-722.226
ATTTTTA753.7232167E-722.229
Produced by FastQC (version 0.11.5)