FastQCFastQC Report
Fri 10 Feb 2017
ERR1631918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60179
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2150.357267485335416No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1770.2941225344389239No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1450.24094783894714106No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1410.2343010020106682No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1080.17946459728476713No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1030.17115605111417603No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1010.16783263264593962No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1010.16783263264593962No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1000.16617092341182138No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1000.16617092341182138No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT910.15121554030475748No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC910.15121554030475748No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC890.14789212183652103No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT880.14623041260240283No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA840.13958357566592997No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT820.13626015719769355No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC810.13459844796357534No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC770.12795161102710248No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA770.12795161102710248No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT760.12628990179298424No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA740.12296648332474783No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT700.11631964638827497No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA680.11299622792003855No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA670.11133451868592034No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG660.10967280945180212No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT660.10967280945180212No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC660.10967280945180212No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA650.10801110021768391No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG640.10634939098356569No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA620.10302597251532926No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC620.10302597251532926No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA610.10136426328121106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG651.8189894E-1234.15384727
GCGGTAA750.032.06666623
CGGTAAT750.032.06666624
GTAATAC705.456968E-1231.71428526
GGTAATA705.456968E-1231.71428525
AGCCGCG850.030.47058919
CGCGGTA801.8189894E-1230.06250222
TATACAC250.005455543829.59999837
GGTATCA1200.029.2916661
TCCGTGC900.028.7777798
CCGCGGT900.028.77777921
GTGCCAG900.028.77777911
GTATCAA1250.028.1199992
ATACGGA803.092282E-1127.75000229
GGAGGGT604.181493E-827.74999833
AACTCCG950.027.2631595
CGTGCCA950.027.26315910
GCCAGCA1050.026.4285713
CGGGCCC358.771649E-426.4285716
CAGCCGC1050.026.4285718