##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631918.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60179
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92743315774606	31.0	31.0	34.0	30.0	34.0
2	32.12068994167401	33.0	31.0	34.0	30.0	34.0
3	32.20851127469715	34.0	31.0	34.0	30.0	34.0
4	35.84991442197444	37.0	35.0	37.0	35.0	37.0
5	35.576812509347114	37.0	35.0	37.0	33.0	37.0
6	35.59037205669752	37.0	35.0	37.0	33.0	37.0
7	35.59773342860466	37.0	35.0	37.0	33.0	37.0
8	35.56740723508201	37.0	35.0	37.0	33.0	37.0
9	37.31808438159491	39.0	37.0	39.0	34.0	39.0
10	37.13159075424982	39.0	37.0	39.0	34.0	39.0
11	37.22727197195035	39.0	37.0	39.0	34.0	39.0
12	37.12913142458333	39.0	37.0	39.0	33.0	39.0
13	37.22890044699978	39.0	37.0	39.0	34.0	39.0
14	38.39708203858489	40.0	38.0	41.0	34.0	41.0
15	38.36559264859835	40.0	38.0	41.0	34.0	41.0
16	38.289087555459545	40.0	38.0	41.0	34.0	41.0
17	38.28391963974144	40.0	38.0	41.0	34.0	41.0
18	38.315492115189684	40.0	38.0	41.0	34.0	41.0
19	38.31105535153459	40.0	38.0	41.0	34.0	41.0
20	38.26932983266588	40.0	38.0	41.0	34.0	41.0
21	38.216936140514136	40.0	38.0	41.0	34.0	41.0
22	38.173150102195116	40.0	38.0	41.0	34.0	41.0
23	38.13355157114608	40.0	38.0	41.0	34.0	41.0
24	38.13667558450622	40.0	38.0	41.0	34.0	41.0
25	38.040296448927364	40.0	38.0	41.0	33.0	41.0
26	37.94896890942023	40.0	37.0	41.0	33.0	41.0
27	37.800312401336015	40.0	37.0	41.0	33.0	41.0
28	37.80669336479503	40.0	37.0	41.0	33.0	41.0
29	37.73834726399575	40.0	37.0	41.0	33.0	41.0
30	37.716130211535585	39.0	37.0	41.0	33.0	41.0
31	37.60954818125924	39.0	37.0	40.0	33.0	41.0
32	37.5690190930391	39.0	37.0	40.0	33.0	41.0
33	37.44003722228685	39.0	37.0	40.0	32.0	41.0
34	37.44731550873228	39.0	37.0	40.0	32.0	41.0
35	37.35077020223002	39.0	36.0	40.0	32.0	41.0
36	37.29609996842753	39.0	36.0	40.0	32.0	41.0
37	37.23504877116602	39.0	36.0	40.0	32.0	41.0
38	37.19558317685571	39.0	36.0	40.0	32.0	41.0
39	37.06592000531747	39.0	36.0	40.0	31.0	41.0
40	36.77345918011267	39.0	35.0	40.0	31.0	41.0
41	36.88288273317935	39.0	35.0	40.0	31.0	41.0
42	36.85971850645574	39.0	35.0	40.0	31.0	41.0
43	36.28199205702986	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	1.0
20	7.0
21	10.0
22	28.0
23	48.0
24	78.0
25	116.0
26	178.0
27	298.0
28	417.0
29	516.0
30	797.0
31	1082.0
32	1471.0
33	1856.0
34	2832.0
35	4147.0
36	6682.0
37	11235.0
38	18153.0
39	10224.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.47827315176391	16.846408215490456	13.262101397497467	27.413217235248176
2	21.617175426643843	20.5270941690623	31.83170208876851	26.02402831552535
3	21.638777646687384	20.10834344206451	29.275993286694696	28.976885624553418
4	15.89923395204307	16.430980906960897	34.62669702055534	33.043088120440686
5	16.64367968892803	33.20427391615015	33.69115472174679	16.460891673175027
6	33.11786503597601	34.95405373967663	15.480483225045283	16.447597999302083
7	29.661509829010118	27.93997906246365	20.006979178783297	22.391531929742932
8	26.949600358929192	30.686784426461056	20.60187108459762	21.761744130012133
9	28.12775220591901	12.506023695973678	17.531032419947156	41.83519167816016
10	19.363897705179546	23.52647933664567	29.458781302447694	27.65084165572708
11	39.55366489971585	19.058143206101796	19.05648149686768	22.331710397314676
12	22.705594974991275	23.98677279449642	26.90639591884212	26.401236311670186
13	35.68520580268865	17.474534305987138	20.442346998122268	26.397912893201948
14	23.955200319048174	20.154871300619817	23.154256468203194	32.735671912128815
15	29.616643679688927	23.97514082985759	19.898968078565613	26.509247411887866
16	25.329433855663936	24.264278236594162	22.446368334468836	27.95991957327307
17	27.248708021070474	23.705943933930442	21.723524817627414	27.321823227371674
18	26.8681766064574	20.994034463849516	23.601256252180995	28.53653267751209
19	28.32051047707672	22.140613835391083	23.471642931919774	26.067232755612423
20	30.18328652852324	20.822878412735342	22.451353462171188	26.542481596570234
21	27.554462520148228	22.298476212632316	21.65373302979445	28.493328237425015
22	28.313863640140248	22.72719719503481	21.347978530716695	27.610960634108245
23	28.46507918044501	22.34832748965586	21.765067548480367	27.421525781418765
24	27.953272736336597	21.593911497366193	22.883397863041925	27.56941790325529
25	28.46341747121089	21.735156782266237	22.984762126323137	26.816663620199737
26	28.61130959304741	22.4796025191512	21.981089748915736	26.927998138885656
27	26.977849415909205	21.61385200817561	24.30582096744712	27.102477608468075
28	26.54414330580435	23.117698865052596	22.0326027351734	28.30555509396966
29	27.285265624221072	23.69763538775985	22.009338805895744	27.00776018212333
30	27.00609847288921	21.96447265657455	23.712590770866914	27.316838099669322
31	28.240748433839048	22.318416723441732	22.594260456305356	26.846574386413863
32	25.753169710364084	22.33835723425115	23.096096645009055	28.812376410375713
33	25.902723541434717	22.10737965070872	24.317452932085942	27.672443875770618
34	26.58402432742319	22.09907110453813	23.440070456471528	27.876834111567156
35	25.542132637631067	23.757456920188105	24.408846939962444	26.29156350221838
36	26.781767726283256	22.029279316705164	24.922315093304974	26.26663786370661
37	27.283603914986955	22.044234699812225	23.988434503730538	26.68372688147028
38	25.975838747735917	22.02097077053457	25.331095564898053	26.67209491683145
39	25.364329749580417	21.32471460143904	25.861180810581764	27.449774838398778
40	25.361006331112186	21.570647568088535	26.876485152627993	26.191860948171293
41	23.42677678259858	21.421093737017895	27.468053639974077	27.684075840409445
42	22.26690373718407	21.522458000299107	28.626264976154474	27.58437328636235
43	21.859784974825107	22.175509729307567	27.944964190166004	28.019741105701325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	5.5
7	8.0
8	8.0
9	8.0
10	12.0
11	16.0
12	16.0
13	24.5
14	33.0
15	61.5
16	90.0
17	88.0
18	86.0
19	86.0
20	111.0
21	136.0
22	116.0
23	96.0
24	117.5
25	139.0
26	139.0
27	168.0
28	197.0
29	254.0
30	311.0
31	377.5
32	444.0
33	444.0
34	567.0
35	690.0
36	802.0
37	914.0
38	1118.0
39	1322.0
40	1322.0
41	1590.5
42	1859.0
43	2187.5
44	2516.0
45	3487.0
46	4458.0
47	4458.0
48	4878.0
49	5298.0
50	6129.0
51	6960.0
52	7107.5
53	7255.0
54	7255.0
55	6651.0
56	6047.0
57	5720.5
58	5394.0
59	5014.5
60	4635.0
61	4635.0
62	4223.5
63	3812.0
64	3087.5
65	2363.0
66	2107.0
67	1851.0
68	1851.0
69	1506.0
70	1161.0
71	995.5
72	830.0
73	666.0
74	502.0
75	502.0
76	410.5
77	319.0
78	252.0
79	185.0
80	144.0
81	103.0
82	103.0
83	85.5
84	68.0
85	55.0
86	42.0
87	30.0
88	18.0
89	18.0
90	12.5
91	7.0
92	5.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	60179.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.9296266139351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.74120898807392	56.97003938250885
2	5.599631468495675	7.271639608501304
3	2.154885601678866	4.197477525382609
4	1.159338690689461	3.0110171322222037
5	0.7728924604596407	2.509180943518503
6	0.4581051338485949	1.7846757174429617
7	0.3531760249782464	1.6052111201581947
8	0.24312842299227108	1.2628990179298427
9	0.20985821774069713	1.226341414779242
>10	1.179812663151968	13.67919041526114
>50	0.10748835542816196	4.669402947872181
>100	0.020473972462507036	1.8129247744229713
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	215	0.357267485335416	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	177	0.2941225344389239	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	145	0.24094783894714106	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	141	0.2343010020106682	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	108	0.17946459728476713	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	103	0.17115605111417603	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	101	0.16783263264593962	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	101	0.16783263264593962	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	100	0.16617092341182138	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	100	0.16617092341182138	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	91	0.15121554030475748	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	91	0.15121554030475748	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	89	0.14789212183652103	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	88	0.14623041260240283	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	84	0.13958357566592997	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	82	0.13626015719769355	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	81	0.13459844796357534	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	77	0.12795161102710248	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	77	0.12795161102710248	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	76	0.12628990179298424	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	74	0.12296648332474783	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	70	0.11631964638827497	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	68	0.11299622792003855	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	67	0.11133451868592034	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	66	0.10967280945180212	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	66	0.10967280945180212	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	66	0.10967280945180212	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	65	0.10801110021768391	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	64	0.10634939098356569	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	62	0.10302597251532926	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	62	0.10302597251532926	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	61	0.10136426328121106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.001661709234118214	0.0
20	0.0	0.0	0.0	0.003323418468236428	0.0
21	0.0	0.0	0.0	0.004985127702354642	0.0
22	0.0	0.0	0.0	0.004985127702354642	0.0
23	0.0	0.0	0.0	0.004985127702354642	0.0
24	0.0	0.0	0.0	0.004985127702354642	0.0
25	0.0	0.0	0.0	0.004985127702354642	0.0
26	0.0	0.0	0.0	0.00830854617059107	0.0
27	0.0	0.0	0.0	0.01994051080941857	0.0
28	0.0	0.0	0.0	0.07477691553531964	0.0
29	0.0	0.0	0.0	0.19940510809418568	0.0
30	0.0	0.0	0.0	0.33898868376011565	0.0
31	0.0	0.0	0.0	0.7560777015237874	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	65	1.8189894E-12	34.153847	27
GCGGTAA	75	0.0	32.066666	23
CGGTAAT	75	0.0	32.066666	24
GTAATAC	70	5.456968E-12	31.714285	26
GGTAATA	70	5.456968E-12	31.714285	25
AGCCGCG	85	0.0	30.470589	19
CGCGGTA	80	1.8189894E-12	30.062502	22
TATACAC	25	0.0054555438	29.599998	37
GGTATCA	120	0.0	29.291666	1
TCCGTGC	90	0.0	28.777779	8
CCGCGGT	90	0.0	28.777779	21
GTGCCAG	90	0.0	28.777779	11
GTATCAA	125	0.0	28.119999	2
ATACGGA	80	3.092282E-11	27.750002	29
GGAGGGT	60	4.181493E-8	27.749998	33
AACTCCG	95	0.0	27.263159	5
CGTGCCA	95	0.0	27.263159	10
GCCAGCA	105	0.0	26.42857	13
CGGGCCC	35	8.771649E-4	26.42857	16
CAGCCGC	105	0.0	26.42857	18
>>END_MODULE