FastQCFastQC Report
Fri 10 Feb 2017
ERR1631916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168086
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46420.3974022460674985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42920.3674386988629262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35260.301861335552348No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA23850.20418017166544244No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20710.17729858931619763No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20360.1743022345957404No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19330.1654843907041091No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18890.16171754476982003No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA17040.14587966981883185No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16730.14322575563785542No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14670.1255900678545929No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14640.1253332374499823No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA13430.11497441113068729No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13400.11471758072607667No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC13270.11360464897276398No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13220.11317659829841295No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT13020.11146439560100883No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12820.1097521929036047No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12670.1084680408805516No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC12620.10803999020620057No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12150.10401631386730086No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11770.10076312874223302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.029.5403211
CTTATAC12400.020.1411337
TACGGCT1202.382876E-1020.04166630
ATACGGC1202.382876E-1020.04166629
GCGGTAA8500.019.37058823
CGCGGTA8700.018.92528722
GTACTGC902.1522774E-618.56
GTTATAG609.23694E-418.51
TTCTGCG6800.017.95588318
CCGCGGT9350.017.80748621
AGCCGCG9400.017.71276719
GTATCAA31150.017.6388442
CGTGCCA9350.017.60962510
TGCGGGT6950.017.56834621
GTAACGT7000.017.44285826
GACCGTT1603.45608E-1117.343757
TATACAC3650.017.23287637
ATACGGT1401.869921E-917.1785726
GCCGTAT650.001579954917.0769231
CGGTAAT9900.016.63131324
Produced by FastQC (version 0.11.5)