##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631916.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1168086
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.870321192104004	31.0	31.0	34.0	30.0	34.0
2	32.06594634299187	33.0	31.0	34.0	30.0	34.0
3	32.148669704114255	34.0	31.0	34.0	30.0	34.0
4	35.81692015827602	37.0	35.0	37.0	35.0	37.0
5	35.49422816470705	37.0	35.0	37.0	33.0	37.0
6	35.51527113585815	37.0	35.0	37.0	33.0	37.0
7	35.52042914648408	37.0	35.0	37.0	33.0	37.0
8	35.48450884609524	37.0	35.0	37.0	33.0	37.0
9	37.20735459546643	39.0	37.0	39.0	34.0	39.0
10	37.03845007987426	39.0	37.0	39.0	33.0	39.0
11	37.13470840331962	39.0	37.0	39.0	34.0	39.0
12	37.052560342303565	39.0	37.0	39.0	33.0	39.0
13	37.140067597762496	39.0	37.0	39.0	34.0	39.0
14	38.27090043027654	40.0	38.0	41.0	34.0	41.0
15	38.24468061426984	40.0	38.0	41.0	34.0	41.0
16	38.18180767511981	40.0	38.0	41.0	34.0	41.0
17	38.15709288528413	40.0	38.0	41.0	33.0	41.0
18	38.1920415106422	40.0	38.0	41.0	34.0	41.0
19	38.211612843574876	40.0	38.0	41.0	34.0	41.0
20	38.19324005253038	40.0	38.0	41.0	34.0	41.0
21	38.144485936823145	40.0	38.0	41.0	34.0	41.0
22	38.105100138174755	40.0	38.0	41.0	34.0	41.0
23	38.03547427158617	40.0	37.0	41.0	34.0	41.0
24	38.04255851024668	40.0	37.0	41.0	34.0	41.0
25	37.97778245779848	40.0	37.0	41.0	33.0	41.0
26	37.86602527553622	39.0	37.0	41.0	33.0	41.0
27	37.72492950005393	39.0	37.0	40.0	33.0	41.0
28	37.7112635542246	39.0	37.0	40.0	33.0	41.0
29	37.64619214681111	39.0	37.0	40.0	33.0	41.0
30	37.58196913583418	39.0	37.0	40.0	33.0	41.0
31	37.47401903626959	39.0	37.0	40.0	33.0	41.0
32	37.40522273188789	39.0	36.0	40.0	32.0	41.0
33	37.30871699515276	39.0	36.0	40.0	32.0	41.0
34	37.26554294803636	39.0	36.0	40.0	32.0	41.0
35	37.163237124663766	39.0	36.0	40.0	32.0	41.0
36	37.107808842842054	39.0	36.0	40.0	32.0	41.0
37	37.04022477797012	39.0	36.0	40.0	31.0	41.0
38	36.99462710793554	39.0	36.0	40.0	31.0	41.0
39	36.89672678210337	39.0	35.0	40.0	31.0	41.0
40	36.576730651681466	39.0	35.0	40.0	31.0	41.0
41	36.61095244699448	39.0	35.0	40.0	31.0	41.0
42	36.55643334480509	38.0	35.0	40.0	31.0	41.0
43	36.000357850363756	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	1.0
16	5.0
17	12.0
18	25.0
19	78.0
20	134.0
21	270.0
22	478.0
23	835.0
24	1450.0
25	2311.0
26	3690.0
27	5540.0
28	8367.0
29	11585.0
30	16319.0
31	21959.0
32	29326.0
33	40252.0
34	57830.0
35	86599.0
36	138431.0
37	234460.0
38	334520.0
39	173606.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.304590586652004	17.939946202591248	13.686149821160429	26.069313389596317
2	20.091414502014405	21.019342753872575	33.95443486181668	24.934807882296337
3	21.05170338485351	21.405444462137204	30.35435747025476	27.18849468275452
4	15.376265103768045	17.291706261354044	34.68528858320363	32.64674005167428
5	16.12064522646449	33.87456060598278	33.824393066948836	16.180401100603895
6	32.064419914287136	35.089625250195624	16.250515801062594	16.595439034454653
7	28.61835515535671	29.557583945017747	20.850433957773657	20.973626941851883
8	26.249351503228358	32.13453461474583	20.745390322287914	20.870723559737897
9	27.64779305633318	13.845299061884141	18.996460877024465	39.510447004758205
10	18.739630472413847	24.88224325948603	30.730185962334964	25.64794030576516
11	36.96303183156035	20.97148668848013	20.1888388354967	21.87664264446282
12	21.80062084469808	25.281015267711453	28.124127846751012	24.79423604083946
13	33.89125458228247	18.780295286477195	22.362223329446635	24.966226801793702
14	23.10343587715288	21.468795961941158	25.14318295057042	30.28458521033554
15	28.147756243975188	25.716856464335674	21.75088135633849	24.38450593535065
16	23.760579272416585	25.49461255421262	24.301806545066032	26.443001628304764
17	24.980438084182158	25.22100256316744	23.87478319233344	25.923776160316965
18	25.11356184390533	22.667937121068142	25.55060158241773	26.667899452608797
19	26.60437673253511	23.784207669640764	25.636040497018197	23.975375100805934
20	27.970115214119506	22.415558443470772	25.080088281171076	24.534238061238643
21	25.86153759226632	23.849100151872378	24.307199983562853	25.982162272298442
22	26.21219670469469	24.318671741635463	24.078792143729146	25.390339409940704
23	25.934306206906	23.53045922988547	24.987286894971774	25.54794766823676
24	26.016663156651138	23.238357449708328	25.32159447163993	25.423384922000608
25	25.879258890184452	23.58405117431422	25.484253727893325	25.052436207608004
26	25.764969360132728	24.135979713822444	24.895427220256042	25.203623705788786
27	25.41602245040177	23.553317135895817	26.011269718154313	25.0193906955481
28	24.894485508772473	24.45025451893097	24.583549498923883	26.07171047337268
29	25.225197459776076	24.797403615829655	24.705800771518536	25.27159815287573
30	25.204822247676965	23.946524485354676	25.83465600991708	25.01399725705128
31	26.01846096948341	23.768284184554904	25.093015411536477	25.12023943442521
32	23.854836030908682	23.99061370481283	25.40412264165481	26.75042762262368
33	24.133411409776333	23.57566138109694	26.75316714693952	25.537760062187203
34	24.497083262704972	23.446047636903447	26.1914790520561	25.865390048335485
35	24.310966829497143	24.478077812763786	26.438892341830993	24.772063015908074
36	24.511465765363166	23.546468325106197	26.790236335338324	25.151829574192313
37	25.024356083370574	23.33081639536815	26.124617536722468	25.52020998453881
38	24.08932219031818	22.94642688980092	27.148343529500394	25.815907390380506
39	23.76194903457451	22.044181678403817	28.08637377727325	26.107495509748425
40	23.616240585025416	22.686771350739583	28.559027331891656	25.13796073234334
41	22.177990319205946	22.35777160243338	29.420350898820807	26.043887179539865
42	21.23687810657777	22.76613194576427	29.730687637725307	26.26630230993266
43	20.484878681877873	23.500067632006548	29.33902127069411	26.676032415421467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	56.5
2	64.0
3	134.0
4	204.0
5	204.0
6	287.0
7	370.0
8	416.0
9	462.0
10	718.0
11	974.0
12	974.0
13	2007.0
14	3040.0
15	5821.5
16	8603.0
17	8395.0
18	8187.0
19	8187.0
20	9165.0
21	10143.0
22	8788.0
23	7433.0
24	7858.5
25	8284.0
26	8284.0
27	9320.0
28	10356.0
29	12958.5
30	15561.0
31	17486.5
32	19412.0
33	19412.0
34	22026.5
35	24641.0
36	26658.0
37	28675.0
38	33417.0
39	38159.0
40	38159.0
41	44555.0
42	50951.0
43	55647.5
44	60344.0
45	76160.5
46	91977.0
47	91977.0
48	100066.0
49	108155.0
50	117903.5
51	127652.0
52	126786.5
53	125921.0
54	125921.0
55	112668.0
56	99415.0
57	91983.5
58	84552.0
59	76826.0
60	69100.0
61	69100.0
62	62115.5
63	55131.0
64	45247.5
65	35364.0
66	30344.0
67	25324.0
68	25324.0
69	21514.5
70	17705.0
71	15002.0
72	12299.0
73	9952.5
74	7606.0
75	7606.0
76	6176.5
77	4747.0
78	3881.5
79	3016.0
80	2388.0
81	1760.0
82	1760.0
83	1412.5
84	1065.0
85	875.5
86	686.0
87	544.5
88	403.0
89	403.0
90	315.0
91	227.0
92	141.0
93	55.0
94	34.5
95	14.0
96	14.0
97	7.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1168086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.20829714360925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.82029079700919	43.21486964777401
2	6.075204755701186	5.97900961653624
3	1.8724968554645776	2.7642714499252463
4	0.9290016758617551	1.8285836205086483
5	0.5752130337523149	1.4152626942880424
6	0.3844357132387809	1.1350456085801572
7	0.28895358531200394	0.9953239720721035
8	0.21411373014810087	0.8428937644523451
9	0.16712258233449903	0.7401435921830999
>10	1.3301786598159377	13.371360980222246
>50	0.19300618998012398	6.575157342663829
>100	0.13232714838145612	12.527032949670094
>500	0.011537109089157378	3.840463858170799
>1k	0.006118163910916791	4.770580902953222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4642	0.3974022460674985	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4292	0.3674386988629262	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3526	0.301861335552348	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2385	0.20418017166544244	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2071	0.17729858931619763	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2036	0.1743022345957404	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1933	0.1654843907041091	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1889	0.16171754476982003	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1704	0.14587966981883185	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1673	0.14322575563785542	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1467	0.1255900678545929	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1464	0.1253332374499823	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1343	0.11497441113068729	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1340	0.11471758072607667	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1327	0.11360464897276398	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1322	0.11317659829841295	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1302	0.11146439560100883	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1282	0.1097521929036047	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1267	0.1084680408805516	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1262	0.10803999020620057	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1215	0.10401631386730086	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1177	0.10076312874223302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.561013487020648E-5	0.0	0.0	0.0	0.0
3	8.561013487020648E-5	0.0	0.0	0.0	0.0
4	8.561013487020648E-5	0.0	0.0	0.0	0.0
5	1.7122026974041295E-4	0.0	0.0	0.0	0.0
6	1.7122026974041295E-4	0.0	0.0	0.0	0.0
7	1.7122026974041295E-4	0.0	0.0	0.0	0.0
8	2.568304046106194E-4	0.0	0.0	0.0	0.0
9	2.568304046106194E-4	0.0	0.0	0.0	0.0
10	3.424405394808259E-4	0.0	0.0	0.0	0.0
11	3.424405394808259E-4	0.0	0.0	0.0	0.0
12	3.424405394808259E-4	0.0	0.0	0.0	0.0
13	3.424405394808259E-4	0.0	0.0	0.0	0.0
14	3.424405394808259E-4	0.0	0.0	2.568304046106194E-4	0.0
15	3.424405394808259E-4	0.0	0.0	5.136608092212388E-4	0.0
16	3.424405394808259E-4	0.0	0.0	0.0011985418881828907	0.0
17	3.424405394808259E-4	0.0	0.0	0.001626592562533923	0.0
18	3.424405394808259E-4	0.0	0.0	0.0017122026974041296	0.0
19	3.424405394808259E-4	0.0	0.0	0.0020546432368849553	0.0
20	3.424405394808259E-4	0.0	0.0	0.0028251344507168137	0.0
21	4.280506743510324E-4	0.0	0.0	0.004708557417861356	0.0
22	4.280506743510324E-4	0.0	0.0	0.006335149980395279	0.0
23	4.280506743510324E-4	0.0	0.0	0.00753369186857817	0.0
24	4.280506743510324E-4	0.0	0.0	0.009673945240333331	0.0
25	4.280506743510324E-4	0.0	0.0	0.011386147937737462	0.0
26	4.280506743510324E-4	0.0	0.0	0.014468112793064895	0.0
27	4.280506743510324E-4	0.0	0.0	0.03184697017171681	8.561013487020648E-5
28	4.280506743510324E-4	0.0	0.0	0.11685783409783183	8.561013487020648E-5
29	4.280506743510324E-4	0.0	0.0	0.26205262283770203	8.561013487020648E-5
30	5.136608092212388E-4	0.0	0.0	0.45715812020690255	8.561013487020648E-5
31	5.136608092212388E-4	0.0	0.0	1.031602125185988	8.561013487020648E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1860	0.0	29.540321	1
CTTATAC	1240	0.0	20.14113	37
TACGGCT	120	2.382876E-10	20.041666	30
ATACGGC	120	2.382876E-10	20.041666	29
GCGGTAA	850	0.0	19.370588	23
CGCGGTA	870	0.0	18.925287	22
GTACTGC	90	2.1522774E-6	18.5	6
GTTATAG	60	9.23694E-4	18.5	1
TTCTGCG	680	0.0	17.955883	18
CCGCGGT	935	0.0	17.807486	21
AGCCGCG	940	0.0	17.712767	19
GTATCAA	3115	0.0	17.638844	2
CGTGCCA	935	0.0	17.609625	10
TGCGGGT	695	0.0	17.568346	21
GTAACGT	700	0.0	17.442858	26
GACCGTT	160	3.45608E-11	17.34375	7
TATACAC	365	0.0	17.232876	37
ATACGGT	140	1.869921E-9	17.178572	6
GCCGTAT	65	0.0015799549	17.076923	1
CGGTAAT	990	0.0	16.631313	24
>>END_MODULE