FastQCFastQC Report
Fri 10 Feb 2017
ERR1631914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361447
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15640.43270520989246No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15220.421085249012995No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14170.3920353468143324No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13510.37377540828945877No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC12930.357728795646388No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA12610.34887549211917657No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12320.34085218579764115No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11500.3181655955091618No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10570.2924356821332035No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10390.287455698899147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10190.2819223841946399No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10110.279709058312837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9870.2730690806674284No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9600.2655991058163438No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9450.26144911978796337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8090.22382257979731468No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7550.20888263009514538No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7540.20860596435992No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7100.19643267201000422No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7050.19504934333387744No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA7000.19366601465775066No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6680.1848127111305392No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6620.18315271671918704No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6570.18176938804306025No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6410.17734273627945452No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6350.17568274186810237No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6270.17346941598629953No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6230.17236275304539808No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6150.1701494271635952No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT6040.16710610407611629No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG5930.16406278098863733No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5810.16074279216593304No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5670.15686947187277803No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5670.15686947187277803No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5590.15465614599097516No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5580.15437948025574982No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5560.15382614878529907No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5520.15271948584439768No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC5510.1524428201091723No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5460.1510594914330455No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5420.14995282849214409No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5420.14995282849214409No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA5380.14884616555124264No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5260.14552617672853835No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5210.14414284805241157No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5200.1438661823171862No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5140.14220618790583406No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5120.14165285643538333No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5070.14026952775925655No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4910.1358428759956508No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4900.13556621026042545No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4860.13445954731952403No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4850.13418288158429867No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4790.13252288717294652No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4660.12892623261501687No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4580.126712906733214No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT4520.12505291232186186No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA4410.1220095892343829No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC4380.12117959202870685No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4310.11924293188212932No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA4280.11841293467645325No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4280.11841293467645325No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT4260.11785960320600256No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4200.1161996087946504No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4150.11481628011852361No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4120.11398628291284753No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT4090.11315628570717146No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4070.11260295423672073No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC4060.11232628850149538No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG4050.11204962276627002No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG4010.11094295982536859No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC3900.10789963673788965No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3810.10540964512086143No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC3790.1048563136504107No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG3780.10457964791518534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGCC308.296212E-637.035
ATGCCCC251.2319518E-436.99999637
CCTAGTT303.5946388E-430.8333342
TATGCCT303.5946388E-430.83333436
CCACTTA303.5946388E-430.83333425
CTAGTTC303.5946388E-430.8333343
GCCTAGT303.5946388E-430.8333341
TGAAACT250.00549142129.59999826
GGTATCA10000.028.491
TATTGAC405.929038E-527.7530
CTTTTTA358.8575843E-426.4285722
TAGTTCC358.8575843E-426.4285724
GCTTTTT358.8575843E-426.4285721
ATACACA706.561095E-926.42857237
ATACGGC1100.025.22727229
AACCCTG751.3671524E-824.66666636
ACCGACC451.3207615E-424.66666632
CGTGTCT451.3207615E-424.66666610
TCCTCCG701.9153413E-723.78571521
CCGCTTA701.9153413E-723.78571525
Produced by FastQC (version 0.11.5)