FastQCFastQC Report
Fri 10 Feb 2017
ERR1631912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110844
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3340.3013243838187002No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3010.2715528129623615No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2790.25170509905813576No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2570.23185738515391No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2540.22915087871242468No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2450.2210313593879687No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2230.20118364548374293No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2150.19396629497311538No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2090.1885532820901447No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2010.18133593157951716No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1890.17050990581357583No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1890.17050990581357583No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1510.136227490888095No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1430.12901014037746744No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC1430.12901014037746744No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1390.12540146512215367No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1330.11998845223918299No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1330.11998845223918299No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1310.1181841146115261No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1290.11637977698386923No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1240.11186893291472699No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1230.11096676410089856No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1200.10826025765941323No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1180.10645592003175634No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1150.103749413590271No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1150.103749413590271No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1110.10014073833495724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTAA200.001834768237.01
TAAACCA200.001834768237.05
TTTATTG403.780042E-837.03
CACCGAA200.001834768237.014
GCTAAAC200.001834768237.03
GGTATCA1500.032.0666661
TATTGAG352.3690252E-531.7142875
GCTTTAT352.3690252E-531.7142871
ACCGAAG303.5787091E-430.83333415
ACCATGC250.005475198429.68
GCACCGA250.005475198429.613
CTAAACC250.005475198429.64
GTACTTA250.005475198429.63
TGCACCG250.005475198429.612
CAATGAG453.965448E-628.77777733
CTTTATT453.965448E-628.7777772
TGTGTGG405.8925572E-527.7514
TCAATGA556.176342E-726.9090932
ACTATCG358.8187E-426.42857211
TATCGGT358.8187E-426.42857213
Produced by FastQC (version 0.11.5)