FastQCFastQC Report
Fri 10 Feb 2017
ERR1631910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560714
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14690.26198739464325843No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13890.247719871449616No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13470.24022942177295376No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12700.22649693069907295No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11710.2088408707469405No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11680.20830583862717894No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10510.18743958595647692No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10230.18244595283870207No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9760.17406378296243719No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9360.166930021365616No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8970.1599746038087153No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8830.1574777872498279No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8410.14998733757316562No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7420.13233127762103317No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6620.11806375442739078No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6480.11556693786850336No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6350.11324846534953648No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5870.10468795143335106No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5850.10433126335350998No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5710.10183444679462256No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5690.10147775871478151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTA251.2328193E-437.06
GGTATCA8000.028.6750011
TAAACCG451.3220313E-424.6666685
TATAAGA451.3220313E-424.66666812
GCGGTAA5050.023.81188223
AATCTAA400.001929960623.12521
CCGAAGT400.001929960623.1254
TAATCTA400.001929960623.12520
CGCGGTA5500.021.86363822
AACTCCG5500.021.8636385
GTAACGT3050.021.83606526
CTTATAC5450.021.38531937
CGGTAAT5900.020.69491624
GACCGTG450.003823420.5555577
GTCGTCT450.003823420.5555573
AGAGCAT450.003823420.5555578
CGTGCCA5900.020.38135510
GCTAACT5900.020.3813552
CTAACTC5950.020.2100833
CCGCGGT5950.020.21008321
Produced by FastQC (version 0.11.5)