##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631910.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560714
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61924795885246	34.0	31.0	34.0	31.0	34.0
2	32.78370613182478	34.0	31.0	34.0	31.0	34.0
3	32.87619356748717	34.0	31.0	34.0	31.0	34.0
4	36.29213288771103	37.0	37.0	37.0	35.0	37.0
5	36.21136622235222	37.0	35.0	37.0	35.0	37.0
6	36.25825465388772	37.0	37.0	37.0	35.0	37.0
7	36.25329847301833	37.0	37.0	37.0	35.0	37.0
8	36.22962151827848	37.0	37.0	37.0	35.0	37.0
9	38.07153914473332	39.0	38.0	39.0	37.0	39.0
10	37.9984287890083	39.0	38.0	39.0	35.0	39.0
11	38.08068284366005	39.0	38.0	39.0	37.0	39.0
12	38.004371212418455	39.0	38.0	39.0	35.0	39.0
13	38.06413251675542	39.0	38.0	39.0	35.0	39.0
14	39.45025271350457	40.0	39.0	41.0	37.0	41.0
15	39.46899310521942	41.0	39.0	41.0	37.0	41.0
16	39.40946899845554	40.0	39.0	41.0	37.0	41.0
17	39.38165802887033	40.0	39.0	41.0	37.0	41.0
18	39.405672053845635	40.0	39.0	41.0	37.0	41.0
19	39.41529014791855	41.0	39.0	41.0	37.0	41.0
20	39.41524377846817	40.0	39.0	41.0	37.0	41.0
21	39.38139407969125	40.0	39.0	41.0	37.0	41.0
22	39.358658781482184	40.0	39.0	41.0	36.0	41.0
23	39.29001416051677	40.0	39.0	41.0	36.0	41.0
24	39.301868688850284	40.0	39.0	41.0	36.0	41.0
25	39.25540471612979	40.0	39.0	41.0	36.0	41.0
26	39.193182264041916	40.0	39.0	41.0	36.0	41.0
27	39.06830398384916	40.0	39.0	41.0	35.0	41.0
28	39.06487799484229	40.0	39.0	41.0	35.0	41.0
29	39.02980307251112	40.0	39.0	41.0	35.0	41.0
30	39.0053824231248	40.0	39.0	41.0	35.0	41.0
31	38.907088105522604	40.0	38.0	41.0	35.0	41.0
32	38.86845700303542	40.0	38.0	41.0	35.0	41.0
33	38.80666792696455	40.0	38.0	41.0	35.0	41.0
34	38.776206764946124	40.0	38.0	41.0	35.0	41.0
35	38.7116212543293	40.0	38.0	41.0	35.0	41.0
36	38.66350581579914	40.0	38.0	41.0	35.0	41.0
37	38.618168977410946	40.0	38.0	41.0	35.0	41.0
38	38.577802587415334	40.0	38.0	41.0	35.0	41.0
39	38.489763052108565	40.0	38.0	41.0	34.0	41.0
40	38.191568250480636	40.0	38.0	41.0	34.0	41.0
41	38.265374147961346	40.0	37.0	41.0	34.0	41.0
42	38.254901785937214	40.0	37.0	41.0	34.0	41.0
43	37.77278612626045	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	3.0
19	13.0
20	10.0
21	36.0
22	91.0
23	129.0
24	250.0
25	411.0
26	714.0
27	1075.0
28	1760.0
29	2714.0
30	3647.0
31	5015.0
32	6865.0
33	9571.0
34	13960.0
35	20915.0
36	32683.0
37	59278.0
38	136357.0
39	265213.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.00284993775793	16.004772486508273	12.790477855020562	28.201899720713236
2	20.986991585728195	19.28630282104602	32.021850711771066	27.704854881454715
3	21.22311909458298	19.368162735369545	29.169416137282106	30.23930203276537
4	15.743498468024697	15.72423731171328	33.98006827009848	34.55219595016354
5	17.128874970127374	32.484475151324915	33.33232271710712	17.05432716144059
6	34.2416989766619	33.35800425885567	15.609205405964538	16.79109135851789
7	30.55336588706542	27.43983563813281	19.78316931626462	22.22362915853715
8	27.892294467411194	30.19400264662555	20.27040523332751	21.643297652635745
9	28.67861333942081	12.468923551043847	17.44472226482663	41.40774084470871
10	19.541691486212223	23.186865318147934	29.282129570511884	27.98931362512796
11	39.655153964409664	19.06961481254258	18.703831186665575	22.571400036382183
12	22.776138994210953	23.51198650292306	26.69935118438277	27.01252331848322
13	35.57678245950699	17.458633099940432	20.481207888513573	26.483376552039005
14	24.183808501303695	19.90408657533074	23.22663603905021	32.685468884315355
15	29.92345473806611	23.86546439004555	19.666889002236434	26.544191869651907
16	25.452369657258423	24.05361735216171	22.332597366928596	28.161415623651276
17	27.19461258324208	23.437082006156437	21.75315758122679	27.615147829374692
18	26.973644317780543	21.232749672738688	23.123553183976146	28.670052825504627
19	28.3397596635718	22.21667374098025	23.039553141173574	26.404013454274374
20	30.281569570226534	20.519016824976728	22.52556561812261	26.673847986674133
21	27.70289309701559	21.993387002999746	21.746202163669892	28.557517736314768
22	28.85392553066269	22.532342691639588	21.18638022235935	27.42735155533837
23	28.279301034038745	21.750839108707826	22.080953926600728	27.8889059306527
24	28.015886887076118	21.208851571389335	22.87672503272613	27.898536508808412
25	28.14982326105644	21.670405946703667	22.871553055568437	27.30821773667146
26	28.056906016257845	22.43229168524417	22.231119608213813	27.279682690284172
27	27.06656156257914	21.51025299885503	24.15830530359506	27.26488013497077
28	26.441465702657684	22.684291813651882	21.907960207877814	28.966282275812627
29	27.593211512464467	23.12105636741726	22.09825329847302	27.18747882164526
30	27.122383247074268	21.944877424141364	23.454203033988808	27.478536294795564
31	28.00839643739946	22.197412584668832	22.664852313300543	27.129338664631163
32	26.17484136297649	21.97787107152666	22.759731342538263	29.087556222958582
33	26.156828614944516	21.88031688169013	24.271553768944596	27.691300734420754
34	26.72057412513331	21.63776898739821	23.580648958292464	28.061007929176014
35	25.890739307383086	23.292088301701046	24.31114614580695	26.506026245108917
36	26.334637622745284	22.001769172876013	24.634483890182874	27.029109314195832
37	26.8855423620598	21.79917034352629	24.459706731060756	26.855580563353154
38	25.95012787267662	21.16783244220761	25.31343965016033	27.56860003495543
39	25.426866459549792	20.32515685358311	26.294688557803088	27.953288129064013
40	25.136700706599086	21.16979422664674	27.30144066315448	26.392064403599697
41	23.464546988304196	20.460519979882793	28.011784974157948	28.063148057655063
42	22.05152716001384	20.898711285967533	29.121084902463647	27.92867665155498
43	21.41483893749755	21.653819950991057	28.742817193792202	28.188523917719195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	4.5
2	6.0
3	12.5
4	19.0
5	19.0
6	26.0
7	33.0
8	37.0
9	41.0
10	59.0
11	77.0
12	77.0
13	182.5
14	288.0
15	588.5
16	889.0
17	839.5
18	790.0
19	790.0
20	995.5
21	1201.0
22	1052.0
23	903.0
24	1024.0
25	1145.0
26	1145.0
27	1310.5
28	1476.0
29	1862.0
30	2248.0
31	2868.5
32	3489.0
33	3489.0
34	4464.0
35	5439.0
36	6400.5
37	7362.0
38	9220.0
39	11078.0
40	11078.0
41	13449.5
42	15821.0
43	18957.0
44	22093.0
45	31538.0
46	40983.0
47	40983.0
48	46126.5
49	51270.0
50	57980.0
51	64690.0
52	65998.0
53	67306.0
54	67306.0
55	61330.5
56	55355.0
57	52852.5
58	50350.0
59	46998.5
60	43647.0
61	43647.0
62	40016.0
63	36385.0
64	30613.5
65	24842.0
66	21401.5
67	17961.0
68	17961.0
69	15247.5
70	12534.0
71	10503.5
72	8473.0
73	6769.5
74	5066.0
75	5066.0
76	4147.0
77	3228.0
78	2578.5
79	1929.0
80	1527.5
81	1126.0
82	1126.0
83	874.0
84	622.0
85	470.5
86	319.0
87	229.0
88	139.0
89	139.0
90	102.5
91	66.0
92	43.0
93	20.0
94	10.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	560714.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.674750874155116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.32343990458405	45.12417002543636
2	6.132099032240023	6.337493796533017
3	1.9852712973426612	3.0776519912337816
4	0.9973770378624206	2.061568398365734
5	0.5987031453100545	1.5468917940735079
6	0.4353646005333168	1.3498414363191122
7	0.30884922008923343	1.1171794554051766
8	0.23919742641763295	0.9888373935896186
9	0.19941609426977225	0.9274299292518761
>10	1.4916773481686316	15.236206925831567
>50	0.15975722139161078	5.677930597794586
>100	0.11634493274889811	11.62936665401411
>500	0.009724352587967602	3.1508763942056435
>1k	0.002778386453705029	1.7745552079458566
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1469	0.26198739464325843	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1389	0.247719871449616	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1347	0.24022942177295376	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1270	0.22649693069907295	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1171	0.2088408707469405	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1168	0.20830583862717894	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1051	0.18743958595647692	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1023	0.18244595283870207	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	976	0.17406378296243719	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	936	0.166930021365616	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	897	0.1599746038087153	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	883	0.1574777872498279	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	841	0.14998733757316562	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	742	0.13233127762103317	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	662	0.11806375442739078	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	648	0.11556693786850336	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	635	0.11324846534953648	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	587	0.10468795143335106	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	585	0.10433126335350998	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	571	0.10183444679462256	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	569	0.10147775871478151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.783440399205299E-4	0.0	0.0	0.0
5	0.0	1.783440399205299E-4	0.0	0.0	0.0
6	0.0	1.783440399205299E-4	0.0	0.0	0.0
7	0.0	1.783440399205299E-4	0.0	0.0	0.0
8	1.783440399205299E-4	1.783440399205299E-4	0.0	0.0	0.0
9	1.783440399205299E-4	1.783440399205299E-4	0.0	0.0	0.0
10	3.566880798410598E-4	1.783440399205299E-4	0.0	0.0	0.0
11	3.566880798410598E-4	1.783440399205299E-4	0.0	0.0	0.0
12	3.566880798410598E-4	1.783440399205299E-4	0.0	0.0	0.0
13	3.566880798410598E-4	1.783440399205299E-4	0.0	0.0	0.0
14	3.566880798410598E-4	1.783440399205299E-4	0.0	0.0	0.0
15	5.350321197615897E-4	1.783440399205299E-4	0.0	0.0	0.0
16	5.350321197615897E-4	1.783440399205299E-4	0.0	0.0	0.0
17	5.350321197615897E-4	1.783440399205299E-4	0.0	0.0012484082794437092	0.0
18	5.350321197615897E-4	3.566880798410598E-4	0.0	0.0016050963592847691	0.0
19	7.133761596821196E-4	3.566880798410598E-4	0.0	0.0016050963592847691	0.0
20	7.133761596821196E-4	3.566880798410598E-4	0.0	0.002318472518966889	0.0
21	8.917201996026494E-4	3.566880798410598E-4	0.0	0.0035668807984105978	0.0
22	8.917201996026494E-4	3.566880798410598E-4	0.0	0.006777073516980136	0.0
23	8.917201996026494E-4	3.566880798410598E-4	0.0	0.008382169876264904	0.0
24	8.917201996026494E-4	5.350321197615897E-4	0.0	0.010700642395231793	0.0
25	0.0010700642395231794	5.350321197615897E-4	0.0	0.011770706634754973	0.0
26	0.0010700642395231794	5.350321197615897E-4	0.0	0.01605096359284769	0.0
27	0.0010700642395231794	5.350321197615897E-4	0.0	0.03370702354498015	0.0
28	0.0010700642395231794	7.133761596821196E-4	0.0	0.12091725906611928	0.0
29	0.0010700642395231794	8.917201996026494E-4	0.0	0.2571721055654041	0.0
30	0.0012484082794437092	8.917201996026494E-4	0.0	0.43408939316656975	0.0
31	0.0012484082794437092	8.917201996026494E-4	0.0	0.9339877370638151	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTTA	25	1.2328193E-4	37.0	6
GGTATCA	800	0.0	28.675001	1
TAAACCG	45	1.3220313E-4	24.666668	5
TATAAGA	45	1.3220313E-4	24.666668	12
GCGGTAA	505	0.0	23.811882	23
AATCTAA	40	0.0019299606	23.125	21
CCGAAGT	40	0.0019299606	23.125	4
TAATCTA	40	0.0019299606	23.125	20
CGCGGTA	550	0.0	21.863638	22
AACTCCG	550	0.0	21.863638	5
GTAACGT	305	0.0	21.836065	26
CTTATAC	545	0.0	21.385319	37
CGGTAAT	590	0.0	20.694916	24
GACCGTG	45	0.0038234	20.555557	7
GTCGTCT	45	0.0038234	20.555557	3
AGAGCAT	45	0.0038234	20.555557	8
CGTGCCA	590	0.0	20.381355	10
GCTAACT	590	0.0	20.381355	2
CTAACTC	595	0.0	20.210083	3
CCGCGGT	595	0.0	20.210083	21
>>END_MODULE