FastQCFastQC Report
Fri 10 Feb 2017
ERR1631909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397012
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72210.2125691637238844No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA62010.18254277582769798No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA56890.16747070660922012No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA56770.16711745498691202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55560.16355550112863892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53970.15887491713305693No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA43660.12852471524975478No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG41350.12172462152032434No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA40860.12028217739589969No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA39990.11772110313416616No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA39430.11607259556339514No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC38090.11212795244762161No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA36480.10738849318165493No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC36480.10738849318165493No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC35180.10356160060665077No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG34970.10294341026761165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35200.031.1661931
TAGATCG450.003827326220.5555559
ATACGGC2800.020.48214129
GCGGTAA23200.020.33405323
CTTATAC30500.020.25901637
GTTATAC851.2467754E-619.5882363
CGCGGTA24500.019.25510222
AACTCCG26050.018.464495
CGTGCCA26650.018.25703410
GTATCAA60750.017.967082
CCGCGGT26750.017.8429921
CTAACTC27000.017.7462963
TAACTCC27500.017.4909084
GGTAATA27250.017.37981625
GCTAACT27600.017.2934782
AGCCGCG27300.017.2802219
CGGTAAT27300.017.1446924
TCCGTGC28500.017.0070178
CTCTATG9550.016.8534051
TCTTATA55850.016.79409237
Produced by FastQC (version 0.11.5)