##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631909.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3397012
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64329475433116	34.0	31.0	34.0	31.0	34.0
2	32.80637159951157	34.0	31.0	34.0	31.0	34.0
3	32.901798992761876	34.0	31.0	34.0	31.0	34.0
4	36.30465832914338	37.0	37.0	37.0	35.0	37.0
5	36.231221732510804	37.0	35.0	37.0	35.0	37.0
6	36.283076715654815	37.0	37.0	37.0	35.0	37.0
7	36.27154746583174	37.0	37.0	37.0	35.0	37.0
8	36.2541710185304	37.0	37.0	37.0	35.0	37.0
9	38.085266110334615	39.0	38.0	39.0	37.0	39.0
10	38.016762966983926	39.0	38.0	39.0	35.0	39.0
11	38.09382480839043	39.0	38.0	39.0	37.0	39.0
12	38.03290509424165	39.0	38.0	39.0	35.0	39.0
13	38.08209920954062	39.0	38.0	39.0	37.0	39.0
14	39.487718029845055	41.0	39.0	41.0	37.0	41.0
15	39.493588483055106	41.0	39.0	41.0	37.0	41.0
16	39.45329483675654	41.0	39.0	41.0	37.0	41.0
17	39.422121264216905	41.0	39.0	41.0	37.0	41.0
18	39.4417800113747	41.0	39.0	41.0	37.0	41.0
19	39.46637221181438	41.0	39.0	41.0	37.0	41.0
20	39.45698425557519	41.0	39.0	41.0	37.0	41.0
21	39.42332850163614	41.0	39.0	41.0	37.0	41.0
22	39.4078478380412	40.0	39.0	41.0	37.0	41.0
23	39.34091107125909	40.0	39.0	41.0	36.0	41.0
24	39.35800933290786	41.0	39.0	41.0	36.0	41.0
25	39.301061050122875	40.0	39.0	41.0	36.0	41.0
26	39.243831049169096	40.0	39.0	41.0	36.0	41.0
27	39.13391533500618	40.0	39.0	41.0	36.0	41.0
28	39.1278968104911	40.0	39.0	41.0	36.0	41.0
29	39.10194665194	40.0	39.0	41.0	35.0	41.0
30	39.05789617463818	40.0	39.0	41.0	35.0	41.0
31	38.9691446482968	40.0	38.0	41.0	35.0	41.0
32	38.92183836854271	40.0	38.0	41.0	35.0	41.0
33	38.868033142067205	40.0	38.0	41.0	35.0	41.0
34	38.83397733066589	40.0	38.0	41.0	35.0	41.0
35	38.76551863814434	40.0	38.0	41.0	35.0	41.0
36	38.711281855936925	40.0	38.0	41.0	35.0	41.0
37	38.666764792117306	40.0	38.0	41.0	35.0	41.0
38	38.625833821016826	40.0	38.0	41.0	35.0	41.0
39	38.550499380043405	40.0	38.0	41.0	35.0	41.0
40	38.25455194152979	40.0	38.0	41.0	34.0	41.0
41	38.31314549374568	40.0	38.0	41.0	34.0	41.0
42	38.28904490181371	40.0	37.0	41.0	34.0	41.0
43	37.82451313095155	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	2.0
13	3.0
14	3.0
15	2.0
16	6.0
17	16.0
18	32.0
19	57.0
20	128.0
21	267.0
22	528.0
23	970.0
24	1543.0
25	2724.0
26	4245.0
27	6680.0
28	10395.0
29	15366.0
30	21832.0
31	29995.0
32	40825.0
33	55389.0
34	81201.0
35	124365.0
36	191177.0
37	352272.0
38	786681.0
39	1670303.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.24055964477017	16.710096991120434	12.597806542926548	27.451536821182852
2	19.74326260843353	19.831899327997665	33.96832274952223	26.45651531404658
3	20.291126436998162	20.515176278447058	29.76047773749401	29.43321954706077
4	14.746842224872916	16.09081745957918	34.749479836986154	34.41286047856175
5	16.05752349417665	33.95295630395182	33.83102561898516	16.15849458288637
6	33.388371898597946	35.02018833021491	15.884371323975305	15.707068447211844
7	30.28873610102054	28.84699847984052	20.26395549971563	20.600309919423303
8	27.317212891800207	31.946604839782726	20.21211582414192	20.524066444275146
9	28.017799171742695	13.542165879896803	17.888367777329016	40.55166717103148
10	18.56193030816494	24.38999332354434	30.69194339025002	26.3561329780407
11	38.20660627633932	20.41441125318368	19.9497381816726	21.429244288804398
12	22.48590820403343	24.111837108611923	27.861838580493682	25.54041610686097
13	33.80255942575416	18.123132917987924	22.255764772099713	25.818542884158198
14	23.615283078187538	19.88415113046407	24.471741636473464	32.02882415487493
15	28.150268530108224	24.988489884639794	21.022210106999918	25.83903147825206
16	24.947983698615136	24.772712018679947	23.04613583937884	27.23316844332608
17	25.969764016141244	24.40774421756532	23.02776675501882	26.594725011274615
18	26.044094045001902	22.398301801701024	24.373567123107012	27.184037030190062
19	27.558984189634888	23.010545738431304	24.301003352357895	25.12946671957591
20	28.517296965686317	21.934600172151292	23.803212941255435	25.74488992090696
21	26.68336173083875	23.08446364039927	23.02776675501882	27.204407873743158
22	27.03043144975643	23.673775659314718	22.56909896108698	26.726693929841872
23	26.87779731128415	23.057940331090972	23.580664419201344	26.483597938423532
24	26.681330534010478	22.5813155796918	23.862647526708766	26.874706359588956
25	27.06855318733051	22.89005749758906	24.06096887499956	25.98042044008087
26	26.879504694125306	23.365004303782268	23.68755247258473	26.067938529507696
27	26.455573309720425	22.804835543707235	24.409745976758398	26.329845169813943
28	25.914539012520414	23.799121109963696	23.304745464543547	26.981594412972342
29	26.248244045060776	24.080397714226503	23.356202450859755	26.315155789852966
30	25.956075515776806	23.701505911665897	24.379219149063942	25.963199423493354
31	26.814565270891006	23.302390453728158	23.668594635520865	26.21444963985997
32	24.613160035937465	23.463237692419103	24.236564368921865	27.687037902721567
33	25.057432826260257	23.03253565191998	25.45822622940396	26.451805292415802
34	25.451808824932026	22.89777015800945	24.839918139824057	26.810502877234466
35	25.159375356931328	24.052343647888204	25.262936957537978	25.525344037642494
36	25.39022529210965	23.30880785820009	25.45916823373011	25.841798615960144
37	26.03596925768882	22.784111448531828	24.879423446252176	26.300495847527177
38	24.99511335255807	22.47086557245014	25.85092428286977	26.68309679212202
39	24.634826135439027	21.631421967305382	26.6858639298301	27.04788796742549
40	24.372595681145665	22.272868038146466	27.45415677071497	25.9003795099929
41	23.296856178311998	21.54137224125202	28.28385651861106	26.87791506182492
42	22.424736798103744	21.864332536947177	28.84364258942859	26.867288075520484
43	21.713611844762397	22.173074454844432	28.923683519516562	27.189630180876605
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	36.0
2	55.0
3	166.5
4	278.0
5	278.0
6	358.5
7	439.0
8	522.5
9	606.0
10	907.0
11	1208.0
12	1208.0
13	2448.0
14	3688.0
15	7937.5
16	12187.0
17	12150.5
18	12114.0
19	12114.0
20	13199.5
21	14285.0
22	13522.0
23	12759.0
24	14473.5
25	16188.0
26	16188.0
27	18611.5
28	21035.0
29	27575.5
30	34116.0
31	40562.0
32	47008.0
33	47008.0
34	56189.5
35	65371.0
36	72832.5
37	80294.0
38	94648.5
39	109003.0
40	109003.0
41	126631.0
42	144259.0
43	158929.0
44	173599.0
45	216112.5
46	258626.0
47	258626.0
48	280472.5
49	302319.0
50	325317.5
51	348316.0
52	354346.0
53	360376.0
54	360376.0
55	328750.0
56	297124.0
57	276876.5
58	256629.0
59	236856.5
60	217084.0
61	217084.0
62	200037.0
63	182990.0
64	155338.0
65	127686.0
66	111901.0
67	96116.0
68	96116.0
69	82867.5
70	69619.0
71	60345.5
72	51072.0
73	41640.0
74	32208.0
75	32208.0
76	26232.0
77	20256.0
78	16552.5
79	12849.0
80	10027.5
81	7206.0
82	7206.0
83	5661.5
84	4117.0
85	3196.5
86	2276.0
87	1667.0
88	1058.0
89	1058.0
90	763.0
91	468.0
92	279.5
93	91.0
94	51.5
95	12.0
96	12.0
97	8.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3397012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.090475746068336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.73293389733819	36.08091957250394
2	8.561769127205414	7.37861409838563
3	2.587670584113329	3.3451186963064967
4	1.2593488292517379	2.1706376073084623
5	0.7326004057659369	1.578405000810846
6	0.47594071637498697	1.2305107137313658
7	0.3453282846545612	1.0416252052037125
8	0.258757760398523	0.8919995998847614
9	0.19653237692718895	0.7621806259168376
>10	1.4726198460022004	12.633401879595906
>50	0.18849455577582777	5.690540463616487
>100	0.16256946830855945	13.861789945969537
>500	0.014602354445317716	4.259451615959773
>1k	0.010420459510214374	8.01898160389251
>5k	4.1133392803477793E-4	1.0558233709140152
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7221	0.2125691637238844	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	6201	0.18254277582769798	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	5689	0.16747070660922012	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5677	0.16711745498691202	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5556	0.16355550112863892	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5397	0.15887491713305693	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	4366	0.12852471524975478	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	4135	0.12172462152032434	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	4086	0.12028217739589969	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	3999	0.11772110313416616	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	3943	0.11607259556339514	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	3809	0.11212795244762161	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	3648	0.10738849318165493	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	3648	0.10738849318165493	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	3518	0.10356160060665077	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	3497	0.10294341026761165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.943763519233962E-5	0.0	0.0	0.0	0.0
3	2.943763519233962E-5	0.0	0.0	0.0	0.0
4	2.943763519233962E-5	0.0	0.0	0.0	0.0
5	2.943763519233962E-5	0.0	0.0	0.0	2.943763519233962E-5
6	2.943763519233962E-5	0.0	0.0	0.0	2.943763519233962E-5
7	2.943763519233962E-5	0.0	0.0	0.0	2.943763519233962E-5
8	1.7662581115403773E-4	0.0	0.0	0.0	2.943763519233962E-5
9	1.7662581115403773E-4	0.0	0.0	0.0	5.887527038467924E-5
10	2.0606344634637735E-4	0.0	0.0	0.0	5.887527038467924E-5
11	2.0606344634637735E-4	0.0	0.0	5.887527038467924E-5	5.887527038467924E-5
12	2.0606344634637735E-4	0.0	0.0	1.1775054076935848E-4	5.887527038467924E-5
13	2.0606344634637735E-4	0.0	0.0	1.7662581115403773E-4	5.887527038467924E-5
14	2.0606344634637735E-4	0.0	0.0	2.943763519233962E-4	5.887527038467924E-5
15	2.0606344634637735E-4	0.0	0.0	5.298774334621132E-4	5.887527038467924E-5
16	2.0606344634637735E-4	0.0	0.0	0.0010891925021165659	5.887527038467924E-5
17	2.0606344634637735E-4	0.0	0.0	0.001560194665194	5.887527038467924E-5
18	2.3550108153871696E-4	0.0	0.0	0.0017662581115403773	5.887527038467924E-5
19	2.649387167310566E-4	2.943763519233962E-5	0.0	0.0022961355450024905	5.887527038467924E-5
20	2.649387167310566E-4	2.943763519233962E-5	0.0	0.002943763519233962	5.887527038467924E-5
21	2.943763519233962E-4	2.943763519233962E-5	0.0	0.004916085077120717	5.887527038467924E-5
22	2.943763519233962E-4	2.943763519233962E-5	0.0	0.0070355948109691695	5.887527038467924E-5
23	2.943763519233962E-4	2.943763519233962E-5	0.0	0.008831290557701887	5.887527038467924E-5
24	2.943763519233962E-4	2.943763519233962E-5	0.0	0.010656423939626942	5.887527038467924E-5
25	2.943763519233962E-4	2.943763519233962E-5	0.0	0.011716178806551169	5.887527038467924E-5
26	2.943763519233962E-4	2.943763519233962E-5	0.0	0.013511874553283886	5.887527038467924E-5
27	2.943763519233962E-4	2.943763519233962E-5	0.0	0.026670497484259696	5.887527038467924E-5
28	2.943763519233962E-4	2.943763519233962E-5	0.0	0.1020897188470338	5.887527038467924E-5
29	2.943763519233962E-4	2.943763519233962E-5	0.0	0.22902480179640225	5.887527038467924E-5
30	3.2381398711573583E-4	2.943763519233962E-5	0.0	0.37859742620867987	5.887527038467924E-5
31	3.2381398711573583E-4	2.943763519233962E-5	0.0	0.8368530932478307	5.887527038467924E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3520	0.0	31.166193	1
TAGATCG	45	0.0038273262	20.555555	9
ATACGGC	280	0.0	20.482141	29
GCGGTAA	2320	0.0	20.334053	23
CTTATAC	3050	0.0	20.259016	37
GTTATAC	85	1.2467754E-6	19.588236	3
CGCGGTA	2450	0.0	19.255102	22
AACTCCG	2605	0.0	18.46449	5
CGTGCCA	2665	0.0	18.257034	10
GTATCAA	6075	0.0	17.96708	2
CCGCGGT	2675	0.0	17.84299	21
CTAACTC	2700	0.0	17.746296	3
TAACTCC	2750	0.0	17.490908	4
GGTAATA	2725	0.0	17.379816	25
GCTAACT	2760	0.0	17.293478	2
AGCCGCG	2730	0.0	17.28022	19
CGGTAAT	2730	0.0	17.14469	24
TCCGTGC	2850	0.0	17.007017	8
CTCTATG	955	0.0	16.853405	1
TCTTATA	5585	0.0	16.794092	37
>>END_MODULE