FastQCFastQC Report
Fri 10 Feb 2017
ERR1631907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578000
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16630.2877162629757785No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA16290.28183391003460206No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15860.27439446366782005No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14250.24653979238754326No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13940.24117647058823527No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13660.23633217993079586No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12490.21608996539792388No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11860.20519031141868513No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11400.19723183391003463No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10690.18494809688581315No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10600.18339100346020762No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9330.16141868512110727No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7870.13615916955017301No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7560.13079584775086506No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6970.12058823529411763No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT6800.1176470588235294No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6790.11747404844290657No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6720.1162629757785467No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT6690.1157439446366782No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT6310.10916955017301039No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT6300.10899653979238755No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6290.10882352941176471No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG6180.1069204152249135No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6010.10397923875432526No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA5940.10276816608996539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.029.51
ACCGCAT358.8640366E-426.4285728
TGATCCG400.001930031523.1250025
CGGTAAC400.001930031523.1250021
GACCGTT753.7341124E-722.27
GCGGTAA6650.021.97744423
TGATATA603.7221624E-521.5833322
GGATATA603.7221624E-521.5833322
TAATACG6850.020.79562227
CGCGGTA7150.020.699322
CGTGCCA7200.020.55555510
ATTGTAC450.003823542520.5555553
AACTCCG7200.020.5555555
ATTTCGG450.003823542520.55555529
TAGCGAA555.138844E-420.18181810
GCTAAAC656.894546E-519.9230773
GGTAATA7350.019.88435425
GATATAG759.252708E-619.7333343
AATACGG7250.019.64827528
AGCCGCG7450.019.61744919
Produced by FastQC (version 0.11.5)