##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631907.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	578000
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62151557093426	34.0	31.0	34.0	31.0	34.0
2	32.7893339100346	34.0	31.0	34.0	31.0	34.0
3	32.88112629757786	34.0	31.0	34.0	31.0	34.0
4	36.26878719723183	37.0	37.0	37.0	35.0	37.0
5	36.2011660899654	37.0	35.0	37.0	35.0	37.0
6	36.25410380622837	37.0	37.0	37.0	35.0	37.0
7	36.258102076124565	37.0	37.0	37.0	35.0	37.0
8	36.23624740484429	37.0	37.0	37.0	35.0	37.0
9	38.079775086505194	39.0	38.0	39.0	37.0	39.0
10	37.99948269896194	39.0	38.0	39.0	35.0	39.0
11	38.08035294117647	39.0	38.0	39.0	37.0	39.0
12	38.0074169550173	39.0	38.0	39.0	35.0	39.0
13	38.07147058823529	39.0	38.0	39.0	37.0	39.0
14	39.46501903114187	40.0	39.0	41.0	37.0	41.0
15	39.474961937716266	41.0	39.0	41.0	37.0	41.0
16	39.41639965397924	40.0	39.0	41.0	37.0	41.0
17	39.38350346020761	40.0	39.0	41.0	37.0	41.0
18	39.40344982698962	41.0	39.0	41.0	37.0	41.0
19	39.423108996539796	41.0	39.0	41.0	37.0	41.0
20	39.41108996539793	41.0	39.0	41.0	37.0	41.0
21	39.37885294117647	40.0	39.0	41.0	37.0	41.0
22	39.35471799307958	40.0	39.0	41.0	36.0	41.0
23	39.289612456747406	40.0	39.0	41.0	36.0	41.0
24	39.30093425605536	40.0	39.0	41.0	36.0	41.0
25	39.24794636678201	40.0	39.0	41.0	36.0	41.0
26	39.17959688581315	40.0	39.0	41.0	36.0	41.0
27	39.0561937716263	40.0	39.0	41.0	35.0	41.0
28	39.05795847750865	40.0	39.0	41.0	35.0	41.0
29	39.021088235294116	40.0	39.0	41.0	35.0	41.0
30	39.00171972318339	40.0	39.0	41.0	35.0	41.0
31	38.910671280276816	40.0	38.0	41.0	35.0	41.0
32	38.86574740484429	40.0	38.0	41.0	35.0	41.0
33	38.80246885813149	40.0	38.0	41.0	35.0	41.0
34	38.784593425605536	40.0	38.0	41.0	35.0	41.0
35	38.71194809688581	40.0	38.0	41.0	35.0	41.0
36	38.67145847750865	40.0	38.0	41.0	35.0	41.0
37	38.622871972318336	40.0	38.0	41.0	35.0	41.0
38	38.57380968858131	40.0	38.0	41.0	35.0	41.0
39	38.48905882352941	40.0	38.0	41.0	34.0	41.0
40	38.19474221453287	40.0	38.0	41.0	34.0	41.0
41	38.263110726643596	40.0	37.0	41.0	34.0	41.0
42	38.25957785467128	40.0	37.0	41.0	34.0	41.0
43	37.781038062283734	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	3.0
18	5.0
19	21.0
20	27.0
21	39.0
22	83.0
23	197.0
24	268.0
25	461.0
26	757.0
27	1141.0
28	1814.0
29	2631.0
30	3752.0
31	5317.0
32	7043.0
33	9907.0
34	14254.0
35	21550.0
36	33919.0
37	60407.0
38	139832.0
39	274570.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.77110726643599	16.05847750865052	12.861764705882353	28.308650519031143
2	20.95968858131488	19.530449826989617	31.933737024221454	27.576124567474047
3	21.169723183391003	19.34152249134948	29.26453287197232	30.224221453287196
4	15.628200692041522	15.838754325259515	34.13044982698962	34.40259515570934
5	16.856747404844292	32.59117647058824	33.67664359861592	16.875432525951556
6	33.9856401384083	33.69117647058823	15.781833910034601	16.541349480968858
7	30.40017301038062	27.474567474048445	20.097577854671282	22.027681660899653
8	27.522491349480973	30.288062283737023	20.649826989619378	21.53961937716263
9	28.67335640138408	12.479065743944636	17.4	41.44757785467128
10	19.398442906574395	23.5121107266436	29.473875432525954	27.615570934256056
11	39.457785467128026	19.055709342560554	18.641176470588235	22.845328719723184
12	22.852249134948096	23.678892733564012	26.51332179930796	26.955536332179932
13	35.63321799307958	17.307093425605537	20.524740484429067	26.534948096885813
14	24.180103806228374	19.948788927335638	22.959169550173012	32.911937716262976
15	29.955536332179932	23.885640138408306	19.638927335640137	26.519896193771626
16	25.582698961937716	24.137370242214534	22.29152249134948	27.988408304498268
17	27.410207612456748	23.629065743944636	21.28304498269896	27.677681660899655
18	27.03546712802768	21.119723183391002	23.02301038062284	28.82179930795848
19	28.280622837370238	22.347404844290658	23.076470588235292	26.295501730103805
20	30.51159169550173	20.447923875432526	22.471626297577853	26.56885813148789
21	27.550865051903113	21.933044982698963	21.714878892733562	28.801211072664362
22	28.90051903114187	22.509342560553634	20.889965397923877	27.700173010380624
23	28.359169550173007	21.788235294117648	22.111937716262975	27.740657439446366
24	28.12249134948097	21.338927335640136	22.775432525951558	27.76314878892733
25	28.21764705882353	21.688408304498267	22.758823529411764	27.335121107266435
26	28.310726643598617	22.475259515570933	21.98356401384083	27.230449826989616
27	27.085294117647056	21.339446366782006	24.05294117647059	27.522318339100345
28	26.466955017301036	22.69273356401384	21.91764705882353	28.922664359861592
29	27.430795847750865	23.215397923875432	22.147231833910034	27.20657439446367
30	27.018166089965394	21.795847750865054	23.360380622837372	27.825605536332183
31	28.336851211072666	22.2621107266436	22.548442906574394	26.85259515570934
32	26.19048442906574	22.05916955017301	22.637543252595155	29.11280276816609
33	26.250346020761246	21.66833910034602	24.272145328719724	27.809169550173014
34	26.77387543252595	21.77993079584775	23.409342560553632	28.036851211072666
35	25.863840830449824	23.652768166089967	24.282525951557094	26.200865051903115
36	26.55311418685121	22.16228373702422	24.60069204152249	26.683910034602075
37	27.174567474048445	22.103633217993078	24.1083044982699	26.613494809688582
38	26.228373702422147	21.673875432525953	25.090657439446368	27.00709342560554
39	25.80536332179931	20.635813148788927	25.78650519031142	27.772318339100345
40	25.48771626297578	21.513321799307956	26.55570934256055	26.44325259515571
41	23.602941176470587	20.817993079584777	27.610553633217993	27.968512110726646
42	22.388754325259516	21.08425605536332	28.598269896193774	27.92871972318339
43	21.575259515570934	21.932179930795847	28.365570934256056	28.126989619377163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.5
2	1.0
3	9.0
4	17.0
5	17.0
6	22.0
7	27.0
8	28.0
9	29.0
10	52.5
11	76.0
12	76.0
13	155.5
14	235.0
15	500.5
16	766.0
17	721.0
18	676.0
19	676.0
20	795.5
21	915.0
22	814.5
23	714.0
24	858.5
25	1003.0
26	1003.0
27	1197.0
28	1391.0
29	2021.5
30	2652.0
31	3194.5
32	3737.0
33	3737.0
34	4817.5
35	5898.0
36	6707.5
37	7517.0
38	9912.5
39	12308.0
40	12308.0
41	14827.0
42	17346.0
43	20362.5
44	23379.0
45	33295.5
46	43212.0
47	43212.0
48	47887.0
49	52562.0
50	60183.0
51	67804.0
52	68950.5
53	70097.0
54	70097.0
55	62741.5
56	55386.0
57	52577.0
58	49768.0
59	46777.0
60	43786.0
61	43786.0
62	40378.5
63	36971.0
64	30895.0
65	24819.0
66	21517.0
67	18215.0
68	18215.0
69	15703.5
70	13192.0
71	11234.0
72	9276.0
73	7446.5
74	5617.0
75	5617.0
76	4556.0
77	3495.0
78	2862.0
79	2229.0
80	1764.0
81	1299.0
82	1299.0
83	1019.0
84	739.0
85	592.0
86	445.0
87	336.5
88	228.0
89	228.0
90	177.0
91	126.0
92	79.5
93	33.0
94	21.5
95	10.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	578000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.27516818331452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.56929562494223	43.522858970550445
2	6.608335145827593	6.64470321736381
3	2.0698029072909208	3.1217906781109317
4	1.0453577669087821	2.102221501722925
5	0.6470597711305479	1.6265519409122648
6	0.426697935827817	1.2871386292329987
7	0.3365234239783515	1.1843140216695536
8	0.24686502715927447	0.9928944607208824
9	0.18954737664885588	0.8576573615754562
>10	1.5445431509237935	15.567092662709342
>50	0.1887534363595636	6.626563904263091
>100	0.11408174698576543	11.027588124507643
>500	0.009333961117017172	2.872084568038721
>1k	0.003802724899525514	2.566539958621954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1663	0.2877162629757785	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1629	0.28183391003460206	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1586	0.27439446366782005	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1425	0.24653979238754326	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1394	0.24117647058823527	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1366	0.23633217993079586	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1249	0.21608996539792388	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1186	0.20519031141868513	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1140	0.19723183391003463	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1069	0.18494809688581315	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1060	0.18339100346020762	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	933	0.16141868512110727	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	787	0.13615916955017301	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	756	0.13079584775086506	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	697	0.12058823529411763	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	680	0.1176470588235294	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	679	0.11747404844290657	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	672	0.1162629757785467	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	669	0.1157439446366782	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	631	0.10916955017301039	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	630	0.10899653979238755	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	629	0.10882352941176471	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	618	0.1069204152249135	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	601	0.10397923875432526	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	594	0.10276816608996539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.4602076124567473E-4	0.0	0.0	0.0	0.0
9	3.4602076124567473E-4	0.0	0.0	0.0	0.0
10	3.4602076124567473E-4	0.0	0.0	0.0	0.0
11	3.4602076124567473E-4	0.0	0.0	0.0	0.0
12	3.4602076124567473E-4	0.0	0.0	0.0	0.0
13	3.4602076124567473E-4	0.0	0.0	0.0	0.0
14	3.4602076124567473E-4	0.0	0.0	0.0	0.0
15	5.190311418685121E-4	0.0	0.0	0.0	0.0
16	5.190311418685121E-4	0.0	0.0	5.190311418685121E-4	0.0
17	5.190311418685121E-4	0.0	0.0	8.650519031141869E-4	0.0
18	5.190311418685121E-4	0.0	0.0	0.0010380622837370243	0.0
19	6.920415224913495E-4	0.0	0.0	0.001384083044982699	0.0
20	8.650519031141869E-4	0.0	0.0	0.0019031141868512112	0.0
21	8.650519031141869E-4	0.0	0.0	0.004152249134948097	0.0
22	8.650519031141869E-4	0.0	0.0	0.006401384083044983	0.0
23	8.650519031141869E-4	0.0	0.0	0.00726643598615917	0.0
24	8.650519031141869E-4	0.0	0.0	0.010207612456747405	0.0
25	8.650519031141869E-4	0.0	0.0	0.011764705882352941	0.0
26	8.650519031141869E-4	0.0	0.0	0.013494809688581315	0.0
27	8.650519031141869E-4	0.0	0.0	0.030103806228373702	0.0
28	8.650519031141869E-4	0.0	0.0	0.10224913494809688	0.0
29	8.650519031141869E-4	0.0	0.0	0.22474048442906575	0.0
30	8.650519031141869E-4	0.0	0.0	0.3866782006920415	0.0
31	8.650519031141869E-4	0.0	0.0	0.8015570934256055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	29.5	1
ACCGCAT	35	8.8640366E-4	26.428572	8
TGATCCG	40	0.0019300315	23.125002	5
CGGTAAC	40	0.0019300315	23.125002	1
GACCGTT	75	3.7341124E-7	22.2	7
GCGGTAA	665	0.0	21.977444	23
TGATATA	60	3.7221624E-5	21.583332	2
GGATATA	60	3.7221624E-5	21.583332	2
TAATACG	685	0.0	20.795622	27
CGCGGTA	715	0.0	20.6993	22
CGTGCCA	720	0.0	20.555555	10
ATTGTAC	45	0.0038235425	20.555555	3
AACTCCG	720	0.0	20.555555	5
ATTTCGG	45	0.0038235425	20.555555	29
TAGCGAA	55	5.138844E-4	20.181818	10
GCTAAAC	65	6.894546E-5	19.923077	3
GGTAATA	735	0.0	19.884354	25
GATATAG	75	9.252708E-6	19.733334	3
AATACGG	725	0.0	19.648275	28
AGCCGCG	745	0.0	19.617449	19
>>END_MODULE