FastQCFastQC Report
Fri 10 Feb 2017
ERR1631905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175104
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8740.4991319444444444No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8440.48199926900584794No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8200.4682931286549708No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7750.442594115497076No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6670.38091648391812866No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6130.350077668128655No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6070.3466511330409357No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5510.3146701388888889No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5240.29925073099415206No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4910.2804047880116959No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4720.26955409356725146No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4630.26441429093567254No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3820.218156067251462No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3740.21358735380116958No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3590.20502101608187137No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3540.20216557017543862No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3520.2010233918128655No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3420.1953125No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3420.1953125No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3310.18903051900584794No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3300.1884594298245614No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3280.1873172514619883No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3150.17989309210526316No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3070.17532437865497075No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2950.16847130847953215No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2870.16390259502923976No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2870.16390259502923976No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2860.16333150584795322No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2780.15876279239766083No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2770.15819170321637427No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2750.15704952485380116No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2720.15533625730994152No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2700.15419407894736842No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2690.15362298976608188No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2640.1507675438596491No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2590.14791209795321636No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2500.14277229532163743No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2450.13991684941520469No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2440.13934576023391812No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2380.13591922514619884No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2360.13477704678362573No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2350.13420595760233917No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2340.13363486842105263No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2340.13363486842105263No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2310.13192160087719298No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2280.13020833333333331No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2230.1273528874269006No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2230.1273528874269006No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2200.12563961988304093No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2170.1239263523391813No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2150.12278417397660818No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2150.12278417397660818No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2150.12278417397660818No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2120.12107090643274854No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2110.12049981725146199No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2110.12049981725146199No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2080.11878654970760233No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG2080.11878654970760233No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC2060.11764437134502924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1990.11364674707602339No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1980.11307565789473685No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC1980.11307565789473685No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1970.1125045687134503No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1960.11193347953216373No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG1910.109078033625731No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1890.10793585526315791No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG1890.10793585526315791No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1880.10736476608187134No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1870.10679367690058479No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.1056514985380117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC200.001837678437.04
CTAAACC303.58714E-430.8333324
GAACGGT303.58714E-430.83333220
TAGGACC303.58714E-430.8333324
GAAGCTT250.005483784229.635
AACGGTA250.005483784229.621
TCGCTTA250.005483784229.613
ACGGTAA250.005483784229.622
GGTATCA4400.028.1704541
TAAAGAC405.9118527E-527.75000237
GCTTAGT358.839279E-426.428577
TAACTGT358.839279E-426.4285735
AGTCGAA358.839279E-426.4285716
TACGCTT400.001924691423.1250024
GCTTAAC400.001924691423.12500215
CTTAGTC400.001924691423.1250028
TCGAACG400.001924691423.12500218
TACACAT400.001924691423.1250025
TATACAC753.703426E-722.237
GTATCAA5850.021.1880342
Produced by FastQC (version 0.11.5)