##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631905.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	175104
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04648094846491	33.0	31.0	34.0	30.0	34.0
2	32.22901247258772	33.0	31.0	34.0	30.0	34.0
3	32.31907894736842	34.0	31.0	34.0	30.0	34.0
4	35.91425095942982	37.0	35.0	37.0	35.0	37.0
5	35.70403303179825	37.0	35.0	37.0	33.0	37.0
6	35.7503083881579	37.0	35.0	37.0	35.0	37.0
7	35.777497944078945	37.0	35.0	37.0	35.0	37.0
8	35.726716694078945	37.0	35.0	37.0	35.0	37.0
9	37.50599643640351	39.0	37.0	39.0	35.0	39.0
10	37.3542694627193	39.0	37.0	39.0	34.0	39.0
11	37.458995796783626	39.0	37.0	39.0	35.0	39.0
12	37.34369289108187	39.0	37.0	39.0	35.0	39.0
13	37.45869311951754	39.0	37.0	39.0	35.0	39.0
14	38.614743238304094	40.0	38.0	41.0	35.0	41.0
15	38.63749543128655	40.0	38.0	41.0	35.0	41.0
16	38.53930235745614	40.0	38.0	41.0	35.0	41.0
17	38.52540775767544	40.0	38.0	41.0	35.0	41.0
18	38.5703125	40.0	38.0	41.0	35.0	41.0
19	38.56470440423977	40.0	38.0	41.0	34.0	41.0
20	38.54954769736842	40.0	38.0	41.0	34.0	41.0
21	38.50398620248538	40.0	38.0	41.0	34.0	41.0
22	38.45030381944444	40.0	38.0	41.0	34.0	41.0
23	38.391904239766085	40.0	38.0	41.0	34.0	41.0
24	38.41287463450293	40.0	38.0	41.0	34.0	41.0
25	38.350848638523395	40.0	38.0	41.0	34.0	41.0
26	38.25099940606725	40.0	38.0	41.0	34.0	41.0
27	38.051272386695906	40.0	38.0	41.0	33.0	41.0
28	38.08732524671053	40.0	38.0	41.0	33.0	41.0
29	38.01946843019006	40.0	38.0	41.0	33.0	41.0
30	37.986562271564324	40.0	37.0	41.0	33.0	41.0
31	37.889157300804094	40.0	37.0	41.0	33.0	41.0
32	37.84402983369883	40.0	37.0	41.0	33.0	41.0
33	37.73736179641813	40.0	37.0	41.0	33.0	41.0
34	37.72938939144737	40.0	37.0	41.0	33.0	41.0
35	37.62115656980994	39.0	37.0	41.0	33.0	41.0
36	37.57110060307018	39.0	37.0	41.0	33.0	41.0
37	37.494466145833336	39.0	37.0	41.0	32.0	41.0
38	37.458893000730995	39.0	36.0	40.0	32.0	41.0
39	37.31913605628655	39.0	36.0	40.0	32.0	41.0
40	37.042397660818715	39.0	36.0	40.0	31.0	41.0
41	37.09147706505848	39.0	36.0	40.0	31.0	41.0
42	37.06023277595029	39.0	35.0	40.0	31.0	41.0
43	36.49066840277778	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	12.0
21	29.0
22	61.0
23	96.0
24	177.0
25	302.0
26	457.0
27	688.0
28	1034.0
29	1450.0
30	2049.0
31	2706.0
32	3838.0
33	5025.0
34	7239.0
35	10583.0
36	16752.0
37	29937.0
38	55510.0
39	37152.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.53072459795322	16.251484831871345	13.113920869883042	28.1038697002924
2	21.823030884502927	20.027526498538013	31.054116410818715	27.095326206140353
3	21.743649488304094	19.168608369883042	29.808570906432745	29.27917123538012
4	15.358872441520468	17.007606907894736	35.77245522660819	31.86106542397661
5	16.59642269736842	32.06323099415205	34.30190058479532	17.038445723684212
6	31.204312865497073	34.850717288011694	16.721491228070175	17.223478618421055
7	28.947939510233915	27.56419042397661	20.427860014619885	23.06001005116959
8	26.539085343567255	29.349986293859647	21.803613852339183	22.30731451023392
9	28.7874634502924	12.038559941520468	17.649511147660817	41.524465460526315
10	19.875045687134502	22.394120065789476	28.55560124269006	29.175233004385966
11	39.90485654239766	18.71345029239766	18.271998355263158	23.10969480994152
12	21.571751644736842	24.216465643274855	26.944558662280706	27.2672240497076
13	37.205317982456144	17.040158991228072	19.490702668128655	26.263820358187136
14	23.63109923245614	20.797925804093566	23.55171783625731	32.019257127192986
15	30.82625182748538	23.458630299707604	19.500411184210524	26.21470668859649
16	23.958333333333336	24.383223684210524	23.234192251461987	28.42425073099415
17	27.378586440058477	23.72361567982456	20.97610562865497	27.921692251461987
18	27.009662828947366	20.25653326023392	22.721354166666664	30.012449744152047
19	28.095303362573098	22.37013432017544	23.259320175438596	26.275242141812864
20	31.675461440058477	19.329655519005847	22.154262609649123	26.84062043128655
21	27.812614217836256	21.427837171052634	21.44782529239766	29.31172331871345
22	29.121550621345026	22.295892726608187	20.745956688596493	27.836599963450293
23	28.86684484649123	21.302768640350877	21.772775036549707	28.057611476608184
24	28.258063779239766	20.393023574561404	23.14110471491228	28.20780793128655
25	28.46137152777778	21.50950292397661	22.834429824561404	27.19469572368421
26	28.687522843567255	22.4992004751462	21.96751644736842	26.84576023391813
27	26.66986476608187	21.1531432748538	25.031409904970758	27.14558205409357
28	26.227270650584796	22.902389437134502	21.278211805555554	29.592128106725145
29	27.473958333333332	23.640807748538013	21.803042763157894	27.082191154970758
30	27.351174159356724	20.763089364035086	23.79671509502924	28.08902138157895
31	28.9273802997076	22.026338633040936	22.39640442251462	26.649876644736842
32	26.64530793128655	21.427266081871345	22.251347770467834	29.67607821637427
33	26.094777960526315	21.210823282163744	24.676192434210524	28.018206323099413
34	26.757241410818715	21.720234831871345	23.118832236842106	28.403691520467838
35	25.279262609649123	24.589957967836256	24.111385233918128	26.01939418859649
36	26.899442616959064	22.279902229532166	24.510576571637426	26.310078581871345
37	27.957099780701753	22.723638523391813	23.557428728070175	25.761832967836256
38	26.71783625730994	22.271906980994153	24.013728983918128	26.99652777777778
39	26.248972039473685	20.94012701023392	25.009137426900587	27.801763523391816
40	26.26667580409357	22.20451845760234	25.550529970760234	25.978275767543856
41	23.66764894005848	21.389574195906434	26.713838633040936	28.22893823099415
42	21.884137426900587	21.877855445906434	28.026772660818715	28.21123446637427
43	21.432976973684212	23.34327028508772	27.107890168128655	28.115862573099413
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.5
4	3.0
5	3.0
6	5.5
7	8.0
8	10.5
9	13.0
10	20.5
11	28.0
12	28.0
13	59.5
14	91.0
15	198.0
16	305.0
17	298.5
18	292.0
19	292.0
20	322.0
21	352.0
22	259.5
23	167.0
24	163.0
25	159.0
26	159.0
27	177.0
28	195.0
29	238.0
30	281.0
31	348.5
32	416.0
33	416.0
34	692.0
35	968.0
36	1060.0
37	1152.0
38	1645.0
39	2138.0
40	2138.0
41	2988.5
42	3839.0
43	4955.5
44	6072.0
45	10375.0
46	14678.0
47	14678.0
48	16233.5
49	17789.0
50	20867.0
51	23945.0
52	23728.0
53	23511.0
54	23511.0
55	20714.0
56	17917.0
57	16935.0
58	15953.0
59	14794.0
60	13635.0
61	13635.0
62	12604.0
63	11573.0
64	9162.5
65	6752.0
66	5773.5
67	4795.0
68	4795.0
69	4071.5
70	3348.0
71	2755.5
72	2163.0
73	1628.5
74	1094.0
75	1094.0
76	890.0
77	686.0
78	530.5
79	375.0
80	295.5
81	216.0
82	216.0
83	161.5
84	107.0
85	77.5
86	48.0
87	37.5
88	27.0
89	27.0
90	17.5
91	8.0
92	5.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	175104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.09036915204678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.92047044194602	37.44174890350877
2	6.520387544686804	5.749725877192982
3	2.3120563701362626	3.0581825657894735
4	1.3496710015025128	2.3802997076023393
5	0.8108388166416248	1.7875091374269008
6	0.49349774623076526	1.3055098684210527
7	0.46111600435210615	1.423154239766082
8	0.31993160976115226	1.128472222222222
9	0.31345526138542046	1.2438322368421053
>10	2.0542977047821354	18.20575201023392
>50	0.255168126003834	7.8907392178362565
>100	0.17745194549505205	14.801489400584794
>500	0.011657427076317289	3.5835846125730995
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	874	0.4991319444444444	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	844	0.48199926900584794	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	820	0.4682931286549708	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	775	0.442594115497076	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	667	0.38091648391812866	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	613	0.350077668128655	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	607	0.3466511330409357	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	551	0.3146701388888889	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	524	0.29925073099415206	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	491	0.2804047880116959	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	472	0.26955409356725146	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	463	0.26441429093567254	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	382	0.218156067251462	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	374	0.21358735380116958	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	359	0.20502101608187137	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	354	0.20216557017543862	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	352	0.2010233918128655	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	342	0.1953125	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	342	0.1953125	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	331	0.18903051900584794	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	330	0.1884594298245614	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	328	0.1873172514619883	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	315	0.17989309210526316	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	307	0.17532437865497075	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	295	0.16847130847953215	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	287	0.16390259502923976	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	287	0.16390259502923976	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	286	0.16333150584795322	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	278	0.15876279239766083	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	277	0.15819170321637427	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	275	0.15704952485380116	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	272	0.15533625730994152	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	270	0.15419407894736842	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	269	0.15362298976608188	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	264	0.1507675438596491	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	259	0.14791209795321636	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	250	0.14277229532163743	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	245	0.13991684941520469	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	244	0.13934576023391812	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	238	0.13591922514619884	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	236	0.13477704678362573	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	235	0.13420595760233917	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	234	0.13363486842105263	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	234	0.13363486842105263	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	231	0.13192160087719298	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	228	0.13020833333333331	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	223	0.1273528874269006	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	223	0.1273528874269006	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	220	0.12563961988304093	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	217	0.1239263523391813	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	215	0.12278417397660818	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	215	0.12278417397660818	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	215	0.12278417397660818	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	212	0.12107090643274854	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	211	0.12049981725146199	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	211	0.12049981725146199	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	208	0.11878654970760233	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	208	0.11878654970760233	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	206	0.11764437134502924	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	199	0.11364674707602339	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	198	0.11307565789473685	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	198	0.11307565789473685	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	197	0.1125045687134503	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	196	0.11193347953216373	No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG	191	0.109078033625731	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	189	0.10793585526315791	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	189	0.10793585526315791	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	188	0.10736476608187134	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	187	0.10679367690058479	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	185	0.1056514985380117	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	5.710891812865497E-4	0.0
16	0.0	0.0	0.0	5.710891812865497E-4	0.0
17	0.0	0.0	0.0	5.710891812865497E-4	0.0
18	0.0	0.0	0.0	0.0011421783625730994	0.0
19	0.0	0.0	0.0	0.0011421783625730994	0.0
20	0.0	0.0	0.0	0.001713267543859649	0.0
21	0.0	0.0	0.0	0.001713267543859649	0.0
22	0.0	0.0	0.0	0.003426535087719298	0.0
23	0.0	0.0	0.0	0.005710891812865497	0.0
24	0.0	0.0	0.0	0.008566337719298246	0.0
25	0.0	0.0	0.0	0.009137426900584795	0.0
26	0.0	0.0	0.0	0.013706140350877192	0.0
27	0.0	0.0	0.0	0.026270102339181287	0.0
28	0.0	0.0	0.0	0.08452119883040936	0.0
29	0.0	0.0	0.0	0.1816063596491228	0.0
30	0.0	0.0	0.0	0.3306606359649123	0.0
31	0.0	0.0	0.0	0.6830226608187134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACC	20	0.0018376784	37.0	4
CTAAACC	30	3.58714E-4	30.833332	4
GAACGGT	30	3.58714E-4	30.833332	20
TAGGACC	30	3.58714E-4	30.833332	4
GAAGCTT	25	0.0054837842	29.6	35
AACGGTA	25	0.0054837842	29.6	21
TCGCTTA	25	0.0054837842	29.6	13
ACGGTAA	25	0.0054837842	29.6	22
GGTATCA	440	0.0	28.170454	1
TAAAGAC	40	5.9118527E-5	27.750002	37
GCTTAGT	35	8.839279E-4	26.42857	7
TAACTGT	35	8.839279E-4	26.42857	35
AGTCGAA	35	8.839279E-4	26.42857	16
TACGCTT	40	0.0019246914	23.125002	4
GCTTAAC	40	0.0019246914	23.125002	15
CTTAGTC	40	0.0019246914	23.125002	8
TCGAACG	40	0.0019246914	23.125002	18
TACACAT	40	0.0019246914	23.125002	5
TATACAC	75	3.703426E-7	22.2	37
GTATCAA	585	0.0	21.188034	2
>>END_MODULE